Wells, Optical Bottom Plates, Cell Culture Inserts

MicroWell® Plates
MicroWell Plates are used in a broad spectrum of applications, including cloning experiments, sample incubations, and in tissue culture based analytical systems. Our MicroWell Plates have excellent cell attachment. All styles are designed to be stackable and are radiation sterilized.
µ 10µl Well Plates
They are Terasaki format plates measuring 56x82mm and having 60 or 72 wells. Wells are shaped like inverted truncated cones, each with a working volume of 10µl. The well surface is Nunclon® treated for good cell attachment. Lids incorporate a stacking feature and are tight-fitting, which reduces evaporation.
µ 96 Well Plates
These plates measure 86x128mm and come in either flat or round bottom wells. Vented lids allow for gas exchange and have condensation rings to control evaporation and retain condensate. Lids are keyed so they cannot be accidentally reversed. Plates have alpha-numeric markings on its two edges. In addition, flat bottom plates have individual well coordinates beside each well for easy well identification when using an inverted microscope. Plates also have raised well rims to provide proper attachment of adhesive sealing film.
| Number of Wells | Well Shape | Volume Well | Culture Area cm2 | Unit Per Sleeve/cs |
| 96 | Round | 0.3ml | 0.36 | 1/50 |
| 96 | Flat | 0.4ml | 0.328 | 1/50 |
| 60 | Conical | 10µl | 0.013 | 10/150 |
| 72 | Conical | 10µl | 0.013 | 10/150 |
Technical Tip
Permanox Plastic; Non Fluorescent

Well Orienter, Stand and Pad
For Easy Pipetting and Identification of Samples in Microplates
The Well Orienter
- Convenient visual aid for pipetting and
identification of samples in microplates. - Sharp, alpha-numeric characters
compatible with 96-, 48- or 24-well
microplates. - No more cross-referencing headaches!
The Well Plate Stand
- View samples at a comfortable 30° angle and pipette without strain
- The neoprene pad and rubber feet prevents slippage of the plate and stand
The Well Pad
- A convenient note pad that corresponds to the layout of a 96-, 48- or 24-well plate
- Keep accurate records of samples in a microplate
- Adhere sheets into a notebook to create a quick reference guide
Ordering
96-well & 384-well Color Orienters
-
Bright colors make it easy to
distinguish between columns
and rows - Fits most standard flat and round bottom 96-well and 384-well plates
- Ideal for use with The
Pipetter Guide for efficient
and stress-free identification
of rows and columns

Adjustable Well Plate Stand
A Convenient, Adjustable-Angle Stand for Pipetting
- Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate
- Convenient knob easily adjusts the angle (45° max. tilt) required to fully view every well
- Compact design takes up little bench-top space measuring only 6˝ L x 4 1⁄2˝ W x 2 1⁄2˝ H
- Durable acrylic material will last for years if properly cared for Adjustable Well Plate Stand shown with a 96-well Color Orienter (sold separately)
| 70493-01 | Adjustable Well Plate Stand | |
|

Well Orienter Stickers
- Permanently label mutliple microplates
- Wells are clearly marked with its corresponding row number and column letter for quick cross-referencing
- Scored paper backing makes aligning quick and easy
The markings of each filled well are easily readable.
| 70490-14 | 96-Well Orienter Stickers | 50/pk | 29.00 |


Cover Slips; Thermanox®; TMX Coverslips
NUNC™ Brand Thernanox®, or TMX coverslips are made from a polymer (in the polyolefin family) that is highly resistant to most chemicals. Thermanox® plastic is resistant to alcohols, aldehydes, hydrocarbons, dilute acids (<10%) and dilute alkalis (<2%). TMX has limited resistance to chlorinated hydro-carbons, however, it is not resistant to concentrated acids or bases.Thermanox® plastic is a flexible, trans-parent polymer that can be sectioned using a microtome and is able to withstand high temp-eratures (temperature range –70ºC to +150ºC). Thermanox® coverslips are culture treated on one side for enhanced cell attachment and growth. The treated side is packaged face up toward the label.
Features:
- Flat and remain flat
- 0.2mm (No. 2) thickness
- Resistant to commonly used solvents
- Safe and easy to handle
- Auto fluorescent in the range 380 to 545 nm
- Thernanox® cover slips can be autoclaved at 20 minutes steam, 20 minutes dry at 120°C

MultiDishes
Nunclon® Surface
Multi Dish Plates are used in a variety of cell biology applications. They all offer excellent cell attachment and growth surface. A certificate of satisfaction comes with each order.
- Useful in all areas of cell culture, including assays and cloning
- Raised well rims to lower risk of cross contamination
- Optically clear and uniformity surface, suitable for microscopy
- Nunclon® certified for consistent cell growth
| Number of Wells | Well Size mm |
Suggested working Vol ml/well | Culture Area cm2/well | Plate size, mm | Units Bag/Case |
| 4 Round | 15 | 0.4-1.0 | 1.9 | 66x66 | 4/120 |
| 4 Rectangle | 24x67 | 3.5-8.0 | 16 | 82x132 | 10/100 |
| 6 Round | 35 | 2.0-5.0 | 9.6 | 88x132 | 5/85 |
| 8 Rectangle | 24x33 | 2.0-4.5 | 8.6 | 82x132 | 10/100 |
| 12 Round | 21 Dia. | 0.7 – 1.8 | 3.5 | 86 x 128 | 1/75 |
| 24 Round | 15 | 0.4-1.0 | 1.9 | 88x132 | 5/85 |
| 48 Round | 12 Dia. | 0.3 – 0.6 | 1.1 | 86 x 128 | 1/75 |
Optical Bottom Microwell® Plates
The NUNC™ Coverglass Optical Bottom 96- and 384- Well Plates have the same quality design of existing NUNC well products, plus an optically clear polystyrene plate bottom. This clear base permits microscopic viewing and reading of well content from the top, bottom or both.
The plate’s upper structure is offered in black (for fluorometric detection) or white (for luminescence detection).
1. 96 Well Optical Bottom Plates
Polystyrene/Coverglass
Sterile. External dimensions 128 x 86 mm.
Features:
- White or black upper structure with #1.5 borosilicate coverglass
- Minimum light scatter and low autofluorescence
- Optical clarity for viewing well contents
- Flat bottom well geometry for plate reader access
- Footprint compatible with standard equipment and automated systems
- CC2™ surface modification closely mimics Poly-D-Lysine and it superior for attachment and growth of fastidious cells
- Non-treated plates are optimized for fluorescence
- Sterile for cell culture
- Working range: 50 – 200 μl/well
| Plate total height | 14.9 mm |
| H2 Vertical distance from inside well bottom to resting plane. | 2.5 mm |
| H3 Inside depth of well | 12.2 mm |
| H4 Coverglass thickness | 0.19 mm |
| H5 Height of flange | 6.2 mm |
| Well diameter | 6.5 mm |
| Total Vol. μl/well | 400 |

96
–Polymer Base
External dimension: 128 x 86 mm
White or black upper structure with Polymer bottom combines the optical clarity of virgin
crystalline polystyrene with optimal surface for a wide range of HTS applications- Optical clarity for viewing well contents
- Footprint compatible with standard equipment and automated systems
- Flat bottom well geometry for plate reader access
- Cell culture, poly-D-Lysine or Collagen I surfaces for cell-based array
- Non-treated plates are optimized for scintillation counting
- Working ranges: 50 –200 μl/well
| Plate total height | 14.9 mm |
| H2 Vertical distance from inside well bottom to resting plane. | 2.55 mm |
| H3 Inside depth of well | 12.2 mm |
| H4 Polystyrene film | 0.25 |
| H5 Height of flange | 6.2 mm |
| Well diameter | 6.5 mm |
| Total Vol. μl/well | 400 |
Lid for the above 96 Well Optical Bottom Plates
Polystyrene
External dimensions 128 x 86 mm
| Cat. # | NUNC # | Description | Color | Sterile | Units /sleeve |
||
| 64811-50 | 255 983 | 96 Well Optical Bottom Plates Lid | clear | No | 20/60 | 80.00 | |
| 64811-51 | 235 304 | 96 Well Optical Bottom Plates Lid | Clear | Yes | 20/60 | 90.00 |

3. 384 Well® Optical Bottom Plates – Polymer Base
- White or black upper structure with optical clear polystyrene bottom
- Can be read from top or bottom
- Rounded square wells eliminate wicking
- Optimized for scintillation counting
- Cell Culture, Collagen I or Poly-D-Lysine surfaces for cell-based arrays
- Non-treated surface for homogeneous assays
- Working range: 10 – 100 μl/well
| H1 Plate total height | 14.4mm |
| H2 Vertical distance from inside well bottom to resting plane | 2.7mm |
| H3 Inside depth of well | 11.6mm |
| H4 Polystyrene film | 2.5mm |
| H5 Height of flange | 2.7mm |
| H7 Bottom Thickness | 0.3mm |
| D1 Well diameter, top | 3.7 x 3.7mm |
| D2 Well distance bottom | 2.9 x 2.9mm |
| F1 Depth of flange | 1.8mm |
| F1 Depth of flange | 1.8mm |
Ordering: External plate dimension 128 x 86 mm
* Certified
Sealing Films
Pre-Cut-Adhesive Films For Microplate Sealing Needs
Sterile –Sterilized by gamma irradiation. Films are also free of cytotoxins.
Prevent Contamination – Films eliminate well-to-well contamination, microbial and viral contamination, evaporation (excluding breathable membranes) and sample spillage.
Ideally Sized and Designed – 6" x 3.25" rectangles fit all microplates. Two scored end-tabs make film positioning and removal hassle-free.
Thermal Stability – Designed to withstand a wide temperature range.
Easy Specimen Retrieval – Pierceable sealing films facilitate easy removal of samples with a pipet tip.
Titer-Tops
| Film Type | Titer-Tops® |
| Ideal Use | Misc Storage |
| Temp. Range | -40°C to 100°C |
| Thickness | 4.4 mil (0.0044 in.) |
| Material | Polyurethane |
| Gas-Permeable | No |
| UV Transparent | To 250nm |
| 70538-05 | Titer-Tops, 6" x 3.25" | 50/pk | 40.00 | |
| 70538-10 | Titer-Tops, 6" x 3.25" | 100/pk | 65.00 |
Breathe-Easy® 
An Adhesive, Gas-Permeable, Cell Culture Membrane
| Film Type | Breathe-Easy* |
| Ideal Use | Cell Culture |
| Temp. Range | -80°C to 100°C |
| Thickness | 1 mil (0.001 in.) |
| Material | Polyurethane |
| Gas-Permeable | Yes |
| UV Transparent | To 300nm |
| 70536-10 | Breathe-Easy, 100/pk | 100/pk | 73.00 |
Breathe-EASIER™
A Rapid Gas Transmissable Sealing Membrane for Cell Growth. Breathe-Easier membranes permit rapid, consistent gas exchange of Oxygen, CO2 and Water Vapor for uniform cell growth.
| Film Type | Breathe-Easier™ |
| Ideal Use | Cell Culture |
| Temp. Range | -20°C to 80°C |
| Thickness | 5.5 mil (0.0055 in.) |
| Material | Non-woven rayon |
| Gas-Permeable | Yes |
| UV Transparent | No |
| 70536-20 | Breathe-EASIER | 100/pk | 60.00 |

AluminaSeal™
Pre-Cut Adhesive Foil Sealing Film
| Film Type | AluminaSeal™ |
| Ideal Use | Cold Storage |
| Temp. Range | -80°C to 110°C |
| Thickness | 2.7 mil (0.0027 in.) |
| Material | Aluminum |
| Gas-Permeable | No |
| UV Transparent | No |
| 70534-10 | AluminaSeal | 100/pk | 60.00 |
HotSeal™
PCR Sealing Film
| Film Type | HotSeal™ |
| Ideal Use | PCR |
| Temp. Range | -40°C to 125°C |
| Thickness | 2.3 mil (0.0023 in.) |
| Material | Polyester |
| Gas-Permeable | No |
| UV Transparent | No |
| 70534-20 | HotSeal | 100/pk | 60.00 |

Microplate Brayer
Uniformly seal your films to microplates with Brayer. It’s perfect for all your sealing films.
| 70534-40 | Microplate Brayer | each | 15.00 |

Breathable Tube Membranes
Remove the top protective layer to expose the breathable membrane
Micro-permeable Membranes for Microtubes
- Ideal for cell growth and freeze drying.
- Permits consistent gas exhange.
- Easy microtube cap closure.
- Will not bind in centrifuge rotors.
- Easy-to-use finger tab for quick liner removal.
- Pre-cut to fit a variety of popular tube sizes.
- Sterile and free of cytotoxins.
| 70537-10 | Breathe-EASY Membranes | 100/pk | 36.00 | |
| 70537-20 | Breathe-EASIER Membranes | 100/pk | 36.00 |
Cell Culture Plates "PTFE"
Manufactured from pure "PTFE" with round bottom or flat bottom distortion free cavities. Totally inert and may be sterilized by all usual means. Special interest in "PTFE" 96 well culture plates arises from the non-stick nature of "PTFE" and the fact that such plates eliminate adherence of cells to the cavity wall and facilitate sample harvesting.
Specifications:
Cavity Diameter: 6.4mm
Cavity Depth: 10.67mm
Overall Length: 128mm
Overall Width: 85mm
Overall Height: 14mm
| Catalog # | Description | Pack | Price | |
| 72268-R | Round Bottom | each | 140.00 | |
| 72268-F | Flat Bottom | each | 140.00 |

CC Inserts — Cell Culture Inserts
NUNC’s CC Inserts with Anopore™ membranes or polycarbonate membranes closely mimic an in-vivo environment, resulting in improved cell attachment and growth. The Anapore™ membranes provide maximum clarity for microscopy along with a high porosity, which promotes higher levels of cell
Features:
- Transparent when wet: This provides optical clarity when viewing cells either microscopically or photographically. Eliminates background interference for better cell differentiation.
- Inserts with a 0.02μm Anapore™ membrane have maximum clarity for microscopy, are nonauto fluorescent and highly porous.
- High porosity: Ensures free diffusion of nutrients to both basolateral (bottom) and spical plasma (top) membranes of attached cells closely resembling an in-vivo environment.
- Solvent resistant: Cells can be fixed/stained without the need for transferring them
- No matrix coating required: very versatile, no coating needed
NUNC TC Inserts are provided sterilized for use in cell and tissue culture applications. These devices can be used without further treatment or may be protein coated using standard protocols.
The TCI is available in different pore sizes and membrane material.
Typical applications for the TCI are:
- Epithelial polarization studies
- Chemotaxix studies
- Co-culture experiments
- Transport studies
- Electron microscopy
- Toxicity testing
- Modeling of in-vivo barriers.
1. Unique Anapore™ Membrane:
The Anapore™ membrane is a rigid inorganic membrane that is highly transparent when wet and highly porous (40-50% surface porosity). This membrane provides maximum clarity for microscopy, is non-autofluorescent, and highly resistant to most solvents. The Anapore™ membrane can be removed from the housing using the removal tool.
Some typical applications are: epithelial polarization studies; transport studies, toxicity testing and
electron microscopy.
2. Polycarbonate Membrane Inserts:
The polycarbonate membrane does not have the transparent characteristics of the Anapore™ membrane. Due to the large pore sizes available with the polycarbonate membrane, it is particularly suitable for chemotaxix studies. These membranes are easily removed (no need for a removal tool) and are ideal for sectioning for transmission microscopy, using glass or diamond knives.
CC Inserts
| TCL Size | 10mm | 25mm | 8 Well Strip |
| Fits in MultiDish | Multi dish 24 | Multi Dish 6 | 96 Flat Well |
| Membrane height above base of well (mm) | 1.0 | 1.0 | 2.0 |
| Culture area (cm2) | 0.5 | 4.2 | 0.08 |
| Dia. of membrane (mm) | 8.0 | 23.0 | 3.2 (base l) |
| Suggested Working Volume, ml* | 0.5 | 1.75 | 60µl |
* Suggested working volume, ml* = In addition to removal well working volume
Anapore® membrane - Sterile
Sorry the 72290-12 and 72290-48 are no longer available. Please see 72296-22 or 72296-24.
Polycarbonate Membrane - Sterile
Sorry the 72296-12 and 72296-48 are no longer available. Please see 72297-12.

Anapore and Polycarbonate Membranes
- Easy cultivation of most cell types without matrix coating
- Treated for cell culture
- Inserts with 0.02 µm Anopore™
membrane have maximum clarity for microscopy, and are non-auto-fluorescent and highly porous - Polycarbonate membranes in larger pore sizes are adaptable to many uses in cell culture, including transport studies, toxicity tests, chemotaxis studies and electron microscopy
- Both membrane types are transparent when wet and suitable for phase contrast and Nomarski Optic Systems
Polycarbonate membrane inserts pre-packed in multidishes
- For multidishes 6, 12, 24
- Treated and tested for cell attachment
- Easy cultivation of most cell types without matrix coating
- Used in a varity of applications, including transport studies, toxicity tests, chemotaxis studies and electron microscopy
- Non-toxic
- Packed in sterile Nunclon™Δ treated multidishes
- Packed in resealable bags
- Non-pyrogenic
- Tabs for better grip/handling
Anapore™ is a trademark of Whatman Scientific Ltd.
Ordering
Nunc CC Inserts - Polycarbonate membranes
Sterile. Pre-packed in multidishes.
| Cat. No. | 72296-16 | 72296-17 | 72296-18 | 72296-19 | 72296-20 | 72296-21 |
| Pore size, µm | 0.4 | 3 | 8 | 0.4 | 3 | 8 |
| Use with Nunc | Multidish 24 | Multidish 24 | Multidish 24 | Multidish 12 | Multidish 12 | Multidish 12 |
| Pore density, pores/cm² | <0.85 X 108 | <1.7 X 106 | <0.85 X 105 | <0.85 X 108 | <1.7 X 106 | <0.85 X 105 |
| Porosity % | <6.8% | <7.7% | <2.7% | <6.8% | <7.7% | <2.7% |
| Culture area, cm² | 0.47 | 0.47 | 0.47 | 1.13 | 1.13 | 1.13 |
| Suggested working volume, ml | 0.5 | 0.5 | 0.5 | 1.1 | 1.1 | 1.1 |
| Units per pack/case | 12/48 | 12/48 | 12/48 | 12/48 | 12/48 | 12/48 |
| Price per Case | 246.00 | 246.00 | 246.00 | 260.00 | 260.00 | 260.00 |
| Add to Cart | Add to Cart | Add to Cart | Add to Cart | Add to Cart | Add to Cart |
All Nunc CC Inserts: Suggested working volume, ml =
In addition to normal well working volume.
Nunc CC Inserts - Polycarbonate membranes Sterile.
Pre-packed in multidishes.
| Cat. No. | 72296-22 |
72296-23 |
72296-24 |
72296-25 |
72296-26 |
72296-27 |
| Pore size, µm | 0.4 |
3 |
8 |
0.4 |
3 |
8 |
| Use with Nunc | Multidish 6 |
Multidish 6 |
Multidish 6 |
Multidish 6 |
Multidish 6 |
Multidish 6 |
| Pore density, pores/cm² | <0.85 X 108 |
<1.7 X 106 |
<0.85 X 105 |
<0.85 X 108 |
<1.7 X 106 |
<0.85 X 105 |
| Porosity % | <6.8% |
<7.7% |
<2.7% |
<6.8% |
<7.7% |
<2.7% |
| Culture area, cm² | 3.14 |
3.14 |
3.14 |
4.1 |
4.1 |
4.1 |
| Suggested working volume, ml | 1.5 |
1.5 |
1.5 |
1.75 |
1.75 |
1.75 |
| Units per pack/case | 6/24 |
6/24 |
6/24 |
6/24 |
6/24 |
6/24 |
| Price per Case | 136.00 |
136.00 |
136.00 |
137.00 |
137.00 |
137.00 |
| Add to Cart | Add to Cart | Add to Cart | Add to Cart | Add to Cart | Add to Cart |
All Nunc CC Inserts: Suggested working volume, ml =

Culture Flasks
Special Culture Flasks made from borosilicate glass and "PTFE"
For all applications where gentle stirring is required, such as biological suspensions and micro-carrier cell cultures. The unique stirring action uses a flexible "PTFE" shaft driven through a fully encapsulated rare-earth magnet to give even and complete mixing throughout the liquid. The height of the shaft is adjustable. Contact materials are "PTFE" and borosilicate glass only. The glass vessel has two side-arms with "PTFE" caps and the base is intended to aid mixing. Recommended for use up to 120 rpm max.









