Tissue Matrices and Sampling Tools
Tissue Matrices; (TM)
The Tissue Matrix is designed to aid the basic research scientist in the free-hand dissection of a wide variety of different tissues and repeatedly slice fresh tissue at intervals as small as 1mm. The TM's are constructed from high grade aluminum to allow for rapid temperature equilibration, ease of cleaning or sterilization and withstand the rigors of daily use.
Features:
- Channels at 1mm intervals
- Channel width is .3mm
- Removable end caps
- Available in 3 basic configurations
Applications:
Biochemical Pharmacology: The reproducible removal of small regions for biochemical analysis, such as the determination of drug and metabolite concentrations. Individual tissues may be either dissected or micropunched from slices formed.
- Anatomy: Precise blocking of tissue prior to microtome sectioning.
- Physiology: Reproducible blocking prior to vibratome sectioning enzymatic dispersion.
Overall Measurements: 90mm(L) x 36mm(W) x 37mm(H)
| Cat. # | Cavity Size, | Tissue Shape | Price | |
| 69010 | TM 10mmx10mm, | Rectangular | 650.00 |  |
| 69011 | TM 15mmx15mm, | Rectangular | 650.00 | |
| 69012 | TM 20mmx20mm, | Rectangular | 650.00 | |
| 69013 | TM 25mmx25mm, | Rectangular | 650.00 | |
| 69014 | TM 10mm Diameter, | V-Shaped Block | 650.00 | |
| 69015 | TM 4mm diameter, | Spherical | 650.00 | |
| 69016 | TM 6mm diameter, | Spherical | 650.00 | |
Rodent Brain Matrices; (RBM)
RBM are designed for similar purposes as the above TM but only for rodent brains. It allows the investigator to slice either coronal (perpendicular to center line) or sagittal (parallel to center line) sections through the brain at intervals as small as 1mm. All RBM's are identical and durable to insure reproducible
Applications:
Biochemical Pharmacology
The reproducible removal of small brain regions (i.e. discrete terminal fields or nuclei) for biochemical analysis, such as the determination of neurotransmitter and metabolite concentrations. Individual brain areas may be either dissected or micropunched from the slices formed.
Anatomy
Precise blocking of the brain prior to microtome sectioning.
Neurophysiology
Reproducible blocking of the brain prior to vibratome sectioning.
Technical Data:
The RBM's are constructed from high grade zinc to allow for rapid temperature equilibration, easy cleaning, and sterilization. The matrices are designed to accommodate the brains of rodents placed ventral side up. The channels are precisely cut at 1mm intervals and 0.3mm wide.
Overall Measurements: 2.75" x 2.75" x 1.5"H
| 69020 | RBM, Gerbil, 70g, Coronal | 650.00 | |
| 69021 | RBM, Gerbil, 70g, Sagittal | 650.00 | |
| 69022 | RBM, Mouse, 30g, Coronal | 575.00 | |
| 69023 | RBM, Mouse, 30g, Sagittal | 575.00 | |
| 69024 | RBM, Rat, 125g-185g, Coronal | 575.00 | |
| 69025 | RBM, Rat, 125g-185g, Sagittal | 575.00 | |
| 69026 | RBM, Rat, 200g-400g, Dorsal-Ventral | 650.00 | |
| 69026-C | RBM Adult Rat, 200g-400g, Coronal | 575.00 | |
| 69026-S | RBM Adult Rat, 200g-400g, Sagital | 575.00 | |
| 69027-C | RBM Adult Guinea Pig, 350g, Coronal | 650.00 | |
| 69027-S | RBM Adult Guinea Pig, 350g, Sagital | 650.00 | |
| 69029-C | RBM Adult Ferret, Coronal | 650.00 | |
| 69029-S | RBM Adult Ferret, Sagital | 650.00 | |
| 69040-C | RBM Adult Rabbit, Coronal | 750.00 | |
| 69040-S | RBM Adult Rabbit, Sagital | 750.00 | |
| 69041-C | RBM Rat Pup, 30g, Coronal | 575.00 | |
| 69041-S | RBM Rat Pup, 30g, Sagital | 575.00 | |
| 69042-C | RBM Hamster, 100g, Coronal | 650.00 | |
| 69042-S | RBM Hamster, 100g, Sagital | 650.00 | |
| 69046-C | Brain Matrix of Monkey, Adult Rhesus, Coronal (40 Slots @ 2mm) | 3,000.00 | |
| 69047-C | Brain Matrix of Adult RetrieverDog, Coronal (40 Slots @ 2mm) | 3,000.00 | |
| 69048-C | Brain Matrix of Adult Cat, Coronal (35 Slots @ 2mm) | 3,000.00 | |
| 69045-C | Brain Matrix, Adult Monkey Cynomogus, Coronal 2mm slots - cerebrum | 3,000.00 | Add to Cart |
69049 |
Cat Heart Matrix, Adult, 2mm Slices | 1,100.00 | Add to Cart |
69050-C |
Rat Heart Matrix, Adult, 1mm Slices | 650.00 | Add to Cart |
69051-C |
Rabbit Heart Matrix, Adult, 2mm Slices | 750.00 | Add to Cart |
*Common animal name, animal weight, slice orientation
Stainless Steel Brain & Heart Matrices
with 0.5mm Thick Slices
When your rodent brain, heart, and other similar organs need to be sliced as thin as possible, these new stainless steel matrices with 0.5mm channel, you can manually achieved these slices - coronal or sagital - with ease.
- Made of high-quality stainless steel
- They can repeatedly use, cleaning, autoclave and frozen
- Produce fine slices
- Accurate sectioning
- Easy cleaning and easy handling.
| Cat. # | Product | Dimensions (mm) | Cavity Depth | Price | |||||
| A | B | C | D | E | |||||
| 69090-C | Mouse Coronal (40-75g) | 3.18 | 11.1 | 8.73 | 19.1 | 12.2 | 7.4 | $475.00 |
Add to Cart |
| 69090-S | Mouse Sagital (40 – 75g) | 3.18 | 11.1 | 8.73 | 19.1 | 12.2 | 7.4 | $475.00 |
Add to Cart |
| 69091-C | Small Rat Coronal (175 – 300g) | 4.76 | 15.9 | 12.7 | 36.6 | 23.8 | 7.61 | $475.00 |
Add to Cart |
| 69091-S | Small Rat Sagital (175 – 300g) | 4.76 | 15.9 | 12.7 | 36.6 | 23.8 | 7.61 | $475.00 |
Add to Cart |
| 69092-C | Large Rat Coronal (300 – 600g) | 4.76 | 19.8 | 14.7 | 36.6 | 24.7 | 10.91 | $475.00 | Add to Cart |
| 69092-S | Large Rat Sagital (300 – 600g) | 4.76 | 19.8 | 14.7 | 36.6 | 24.7 | 10.91 | $475.00 |
Add to Cart |
| 69095-H | Mouse Heart | 8.3 | 12.1 | - | - | - | 4.8 | $475.00 |
Add to Cart |
| 69096-H | Rat Heart | 12.7 | 19.8 | - | - | - | 9.6 | $475.00 | Add to Cart |
Brain Matrix Razor Blades
These razor blades are specifically made to work and fit all of our Brain Matrices. They measure 118mmx19mmx.2286mm (4.65x.75x.0089"). They are packed 25 blades in a box.
| 70933-70 | Brain Matrix Single Razor Blade (118mm L x 19mm W x 0.2286mm Thick) | 25/pk | 50.00 | |
Acrylic Matrices: The Economy Rodent Brain Matrix
Features:
- Highly polished
- Precision machined
- Precise blocking of the brain
- Reproducible 1 mm segments
- Cost savingand in all directions
Low cost acrylic matrices are precisely machined and highly polished to ensure reproducible results. These matrices allow the investigator to slice either coronal (perpendicular to center line) or sagittal (parallel to center line) sections through the brain at 1 mm intervals.
| Type | Size (g) | Dimensions (mm) | Cavity | ||||
| A | B | C | D | E | Depth | ||
| Mouse Coronal | 40-75 | 3.18 | 11.1 | 8.73 | 19.1 | 12.2 | 7.4 |
| Mouse Sagittal | 40-75 | 3.18 | 11.1 | 8.73 | 19.1 | 12.2 | 7.7 |
| Small Rat Coronal | 175-300 | 4.76 | 15.9 | 12.7 | 36.6 | 23.8 | 7.61 |
| Small Rat Sagittal | 175-300 | 4.76 | 15.9 | 12.7 | 36.6 | 23.8 | 7.61 |
| Large Rat Coronal | 300-600 | 4.76 | 19.8 | 14.7 | 36.6 | 24.7 | 10.91 |
| Large Rat Sagittal | 300-600 | 4.76 | 19.8 | 14.7 | 36.6 | 24.7 | 10.91 |
| 69080-C | Mouse, Coronal (40-75 g) | each | $255.00 | |
| 69080-S | Mouse, Sagittal (40-75 g) | each | $255.00 | |
| 69081-C | Small Rat, Coronal (175-300 g) | each | $265.00 | |
| 69081-S | Small Rat, Sagittal (175-300 g) | each | $265.00 | |
| 69083-C | Large Rat, Coronal (300-600 g) | each | $265.00 | |
| 69083-S | Large Rat, Sagittal (300-600 g) | each | $265.00 | |
Brain Blocker One™
A Perfect Cryosectioning Method - Brain Blocker One™ with these features:
- Easily reproducible alignment in atlas planes
- Section one or multiple brains with each knife pass
- Time saving in Tissue sectioning
- Time saving in Microscope analysis
Brain Blocker One is a set of gel blocks, left and right half, with an internal cavity cast originally an actual mouse brain. Drop a fresh or fixed brain in to displace the liquid in the one half cavity, add another half on top and the brain is now fully encased, as if it had been embedded. The brain cavity is in sterotaxic orientation, top surface of the gel parallel to skull flat. The gel expands slowly with hydration, and the cavity accommodates different brain sizes simply by the length of the presoak time, 1 to 4 hours for fresh adult brain, less for fixed or young brain, more for aged brain.
The entire block can be frozen and sectioned. The cryostat pedestal need never be readjusted from parallel to the blade plane of motion, and every brain will come out sectioned in the same plane as the atlas.
Multi-blocks can be frozen and mounted on the same pedestal together. Each pass of the cryostat knife, produces the same thickness sections, same orientation from every brain are mounted on that pedestal. Such large set of the sections can be transferred intact from the blade to the slide using our Cryo Jane Tape Transfer System (#62800-10). Up to 24 brains can be sectioned with each pass of the knife using the whole body cryostat and Macro Tape Transfer system.
Gels will come in packages of 12 pairs, slightly dehydrated. Soak in water, saline, or buffer to fit the brains
| 69100 | Brain Blocker One™ Mouse Brain | 12/pk | 75.00 | |
| 69102 | Brain Blocker One™ Mouse Brain | 12/pk | 75.00 | |
Brain Blocker One ™ is trade mark of Mc Cormick
Sampling
Tools – Harris Micro-Punch™ and Harris Uni-Cores™
The Harris Micro-Punch™ and Uni-Cores are designed to serve research scientists with a dissection tool for small pre-defined regions of the tissue or the specimen.
Applications
- Forensic - Punch cut, retrieve, store and preferentially eject cored samples from source materials such as paper, gels, cloth, leaves, paint chip, films, etc.
- Electrophysiology – Punch dissection of discrete regions prior to acute dissociation for patch recording
- Biomedical Pharmacology – Punch removal of discrete brain
regions for analysis of neurotransmitters and metabolite changes
in response to different pharmacological agents.
• Anatomy – Punch out small brain regions for analysis of neurotransmitter
concentrations of mRNA levels or for subsequent use in electron
microscopy.
• Others – Positioning samples inside the quartz pyrolysis gel tubes, or on FTIR diamond cell sample stages.
Operating
- For most forensic samples, position source of materials (i.e. blood cards, paint chips, paper, leaves, gels, etc.) on the self-healing cutting mat. Remove the protective cover cap from the punch. Firmly grip the punch. The tip is then positioned at a right angle above the target area – DO NOT DEPRESS THE PLUNGER. The tip is pushed downward into the source material until it makes contact with the cutting mat. Lift the Harris Micro-Punch™ away from the source material with the cored sample stored in the cutting tip.
- For most biology samples – Typically tissue to be punched is first sliced into 1-2mm thick sections. (Our Tissue and Brain Matrices are specially designed for this purpose See page 198 of Catalog XIV). The section to be punched is then placed on a saline-soaked filter paper disc (EMS #70662 – Petri dish with absorbent paper) on an ice-cold dissection plate (EMS #62536-01 – Cold Plate) Then follow the above procedure.
Sample Storage and Ejection
After coring, the sample is temporarily stored in the hollow cutting tip ready for the preferred ejection. The Harris MicroPunch™ can be rested on its side without loss of sample from the hollow tip. To eject sample, position tip over target area and depress plunger.
Cleaning
Clean the tip between each sample extraction by coring blank filter paper, rinsing with ethanol or spraying with compressed air to remove dried artifacts. Rinse mat with ethanol after each sample extraction.
A. Harris Micro-Punch
The tips and plungers are made from high-grade 440c stainless steel. Tips are heat-treated to a Rockwell hardness of Rc-58 and then individually sharpened to a razor edge for a long cutting life. Tips are replaceable and available in sizes 0.50, 1.0, 1.20, 2.0, and 3.0 mm. The barrel and knob screw assembly are constructed from injected molded, high-tech, lubricated plastic to eliminate sticking. Length: 140mm (5 ½”). Sterilization temperature up to 180°C (360°F)
| 69034-05 | Harris Micro-Punch, 0.5mm | each | 99.00 | |
| 69034-10 | Harris Micro-Punch, 1.0mm | each | 99.00 | |
| 69034-12 | Harris Micro-Punch, 1.2mm | each | 99.00 | |
| 69034-20 | Harris Micro-Punch, 2.0mm | each | 99.00 | |
| 69034-30 | Harris Micro-Punch, 3.0mm | each | 99.00 | |
| 69035-05 | Replacement Tip, 0.5mm | each | 34.00 | |
| 69035-10 | Replacement Tip, 1.0mm | each | 34.00 | |
| 69035-12 | Replacement Tip, 1.2mm | each | 34.00 | |
| 69035-20 | Replacement Tip, 2.0mm | each | 34.00 | |
| 69035-30 | Replacement Tip, 3.0mm | each | 34.00 | |
B. Harris Uni-Cores
The Harris Uni-Core™ is a disposable multi-purpose sampling tool for soft substrates. They are made from polypropylene, including a hollow body with built-in tool steel razor sharp cutting tip, with a plunger for the expelling of the punched sample. Uni-Core comes individually wrapped and sterilized by ethylene oxide. Uni-Core can also be reusable by rinsing the tip with ethanol after each use and autoclaving for 20 minutes at 250°F/15 psi. Available sizes: 0.5, 0.75, 1.0, 1.25, 2.0, 2.5, 3.0, 3.5, 4.0, 5.0, 6.0, 7.0, and 8.0mm diameter.
| 69036-05 | Harris Uni-Core™, Size 0.5mm | each | 5.50 | |
| 69036-07 | Harris Uni-Core™, Size 0.75mm | each | 5.50 | |
| 69036-10 | Harris Uni-Core™, Size 1.0mm | each | 5.50 | |
| 69036-12 | Harris Uni-Core™, Size 1.25mm | each | 5.50 | |
| 69036-20 | Harris Uni-Core™, Size 2.0mm | each | 5.50 | |
| 69036-25 | Harris Uni-Core™, Size 2.5mm | each | 5.50 | |
| 69036-30 | Harris Uni-Core™, Size 3.0mm | each | 5.50 | |
| 69036-35 | Harris Uni-Core™, Size 3.5mm | each | 5.50 | |
| 69036-40 | Harris Uni-Core™, Size 4.0mm | each | 5.50 | |
| 69036-50 | Harris Uni-Core™, Size 5.0mm | each | 5.50 | |
| 69036-60 | Harris Uni-Core™, Size 6.0mm | each | 5.50 | |
| 69036-70 | Harris Uni-Core™, Size 7.0mm | each | 5.50 | |
| 69036-80 | Harris Uni-Core™, Size 8.0mm | each | 5.50 | |
C. Cutting Mats
These cutting mats are inert, self-healing and can be sterilized with ethanol. Available sizes 6 x 8” and 2 ½ x 3”
| 69037 | Cutting Mat, Size 6 x 8” | each | 7.20 | |
| 69037-23 | Cutting Mat, Size 2½ x 3” | each | 3.00 | |
