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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow14Tissue Matrices and Sampling Tools

Tissue MatricesTissue Matricesarrow12Tissue Matrices; (TM)

The Tissue Matrix is designed to aid the basic research scientist in the free-hand dissection of a wide variety of different tissues and repeatedly slice fresh tissue at intervals as small as 1mm. The TM's are constructed from high grade aluminum to allow for rapid temperature equilibration, ease of cleaning or sterilization and withstand the rigors of daily use.

Features:

  • Channels at 1mm intervals
  • Channel width is .3mm
  • Removable end caps
  • Available in 3 basic configurations

Applications:

Biochemical Pharmacology: The reproducible removal of small regions for biochemical analysis, such as the determination of drug and metabolite concentrations. Individual tissues may be either dissected or micropunched from slices formed.

  • Anatomy: Precise blocking of tissue prior to microtome sectioning.
  • Physiology: Reproducible blocking prior to vibratome sectioning enzymatic dispersion.

Overall Measurements: 90mm(L) x 36mm(W) x 37mm(H)

Cat. # Cavity Size, Tissue Shape Price  
69010 TM 10mmx10mm, Rectangular 675.00 Add to Cart
69011 TM 15mmx15mm, Rectangular 675.00 Add to Cart
69012 TM 20mmx20mm, Rectangular 675.00 Add to Cart
69013 TM 25mmx25mm, Rectangular 675.00 Add to Cart
69014 TM 10mm Diameter, V-Shaped Block 675.00 Add to Cart
69015 TM 4mm diameter, Spherical 675.00 Add to Cart
69016 TM 6mm diameter, Spherical 675.00 Add to Cart

Rodent Brain MatricesRodent Brain Matricesarrow12Rodent Brain Matrices; (RBM)

RBM are designed for similar purposes as the above TM but only for rodent brains. It allows the investigator to slice either coronal (perpendicular to center line) or sagittal (parallel to center line) sections through the brain at intervals as small as 1mm. All RBM's are identical and durable to insure reproducible

Applications:

Biochemical Pharmacology

The reproducible removal of small brain regions (i.e. discrete terminal fields or nuclei) for biochemical analysis, such as the determination of neurotransmitter and metabolite concentrations. Individual brain areas may be either dissected or micropunched from the slices formed.

Anatomy

Precise blocking of the brain prior to microtome sectioning.

Neurophysiology

Reproducible blocking of the brain prior to vibratome sectioning.

Technical Data:

The RBM's are constructed from high grade zinc to allow for rapid temperature equilibration, easy cleaning, and sterilization. The matrices are designed to accommodate the brains of rodents placed ventral side up. The channels are precisely cut at 1mm intervals and 0.3mm wide.

Overall Measurements: 2.75" x 2.75" x 1.5"H

69020 RBM, Gerbil, 70g, Coronal each 675.00 Add to Cart
69021 RBM, Gerbil, 70g, Sagittal each 675.00 Add to Cart
69022 RBM, Mouse, 30g, Coronal each 598.00 Add to Cart
69023 RBM, Mouse, 30g, Sagittal each 598.00 Add to Cart
69024 RBM, Rat, 125g-185g, Coronal each 598.00 Add to Cart
69025 RBM, Rat, 125g-185g, Sagittal each 598.00 Add to Cart
69026 RBM, Rat, 200g-400g, Dorsal-Ventral each 675.00 Add to Cart
69026-C RBM Adult Rat, 200g-400g, Coronal each 598.00 Add to Cart
69026-S RBM Adult Rat, 200g-400g, Sagital each 598.00 Add to Cart
69027 RBM Guinea Pig,250-450g, Coronal each 675.00 Add to Cart
69028 RBM Guinea Pig,250-450g, Sagital each 675.00 Add to Cart
69027-C RBM Adult Guinea Pig, 350g, Coronal each 675.00 Add to Cart
69027-S RBM Adult Guinea Pig, 350g, Sagital each 675.00 Add to Cart
69029-C RBM Adult Ferret, Coronal each 675.00 Add to Cart
69029-S RBM Adult Ferret, Sagital each 675.00 Add to Cart
69040-C RBM Adult Rabbit, Coronal each 775.00 Add to Cart
69040-S RBM Adult Rabbit, Sagital each 775.00 Add to Cart
69041-C RBM Rat Pup, 30g, Coronal each 598.00 Add to Cart
69041-S RBM Rat Pup, 30g, Sagital each 598.00 Add to Cart
69042-C RBM Hamster, 100g, Coronal each 675.00 Add to Cart
69042-S RBM Hamster, 100g, Sagital each 675.00 Add to Cart
69046-C Brain Matrix of Monkey, Adult Rhesus, Coronal (40 Slots @ 2mm) each 3,150.00 Add to Cart
69047-C Brain Matrix of Adult RetrieverDog, Coronal (40 Slots @ 2mm) each 3,150.00 Add to Cart
69048-C Brain Matrix of Adult Cat, Coronal (35 Slots @ 2mm) each 3,150.00 Add to Cart
69045-C Brain Matrix, Adult Monkey Cynomogus, Coronal 2mm slots - cerebrum each 3,150.00 Add to Cart

69049

Cat Heart Matrix, Adult, 2mm Slices each 1,100.00 Add to Cart

69050-C

Rat Heart Matrix, Adult, 1mm Slices each 675.00 Add to Cart

69051-C

Rabbit Heart Matrix, Adult, 2mm Slices each 750.00 Add to Cart

*Common animal name, animal weight, slice orientation

Rodent Brain Matricesarrow12Stainless Steel Brain & Heart Matrices

with 0.5mm Thick Slices

When your rodent brain, heart, and other similar organs need to be sliced as thin as possible, these new stainless steel matrices with 0.5mm channel, you can manually achieved these slices - coronal or sagital - with ease.

  • Made of high-quality stainless steel
  • They can repeatedly use, cleaning, autoclave and frozen
  • Produce fine slices
  • Accurate sectioning
  • Easy cleaning and easy handling.Rodent Brain Matrices

 

Cat. # Product Dimensions (mm) Cavity Depth Price  
A B C D E
69090-C Mouse Coronal (40-75g) 3.18 11.1 8.73 19.1 12.2 7.4 365.00 Add to Cart
69090-S Mouse Sagital (40 – 75g) 3.18 11.1 8.73 19.1 12.2 7.4 365.00 Add to Cart
69091-C Small Rat Coronal (175 – 300g) 4.76 15.9 12.7 36.6 23.8 7.61 365.00 Add to Cart
69091-S Small Rat Sagital (175 – 300g) 4.76 15.9 12.7 36.6 23.8 7.61 365.00 Add to Cart
69092-C Large Rat Coronal (300 – 600g) 4.76 19.8 14.7 36.6 24.7 10.91 365.00 Add to Cart
69092-S Large Rat Sagital (300 – 600g) 4.76 19.8 14.7 36.6 24.7 10.91 365.00 Add to Cart
69095-H Mouse Heart 8.3 12.1 - - - 4.8 365.00 Add to Cart
69096-H Rat Heart 12.7 19.8 - - - 9.6 365.00 Add to Cart

arrow10Brain Matrix Razor Blades

These razor blades are specifically made to work and fit all of our Brain Matrices. They measure 118mmx19mmx.2286mm (4.65x.75x.0089"). They are packed 25 blades in a box.

70933-70 Brain Matrix Single Razor Blade (118mm L x 19mm W x 0.2286mm Thick) 25/pk 50.00 Add to Cart

Acrylic MatricesAcrylic Matricesarrow10 Acrylic Matrices

Features:

  • Highly polished
  • Precision machined
  • Precise blocking of the brain
  • Reproducible 1 mm segments
  • Cost savingand in all directions

Low cost acrylic matrices are precisely machined and highly polished to ensure reproducible results. These matrices allow the investigator to slice either coronal (perpendicular to center line) or sagittal (parallel to center line) sections through the brain at 1 mm intervals.

Type Size (g) Dimensions (mm) Cavity
    A B C D E Depth
Mouse Coronal 40-75 3.18 11.1 8.73 19.1 12.2 7.4
Mouse Sagittal 40-75 3.18 11.1 8.73 19.1 12.2 7.7
Small Rat Coronal 175-300 4.76 15.9 12.7 36.6 23.8 7.61
Small Rat Sagittal 175-300 4.76 15.9 12.7 36.6 23.8 7.61
Large Rat Coronal 300-600 4.76 19.8 14.7 36.6 24.7 10.91
Large Rat Sagittal 300-600 4.76 19.8 14.7 36.6 24.7 10.91
69080-C Mouse, Coronal (40-75 g) each 168.00 Add to Cart
69080-S Mouse, Sagittal (40-75 g) each 168.00 Add to Cart
69081-C Small Rat, Coronal (175-300 g) each 168.00 Add to Cart
69081-S Small Rat, Sagittal (175-300 g) each 179.00 Add to Cart
69082-S Rat, Sagittal (175-300 g) each 168.00 Add to Cart
69083-C Large Rat, Coronal (300-600 g) each 168.00 Add to Cart
69083-S Large Rat, Sagittal (300-600 g) each 168.00 Add to Cart

mouse branin blockTissue Freezing MediumBrain Blocker One™

A Perfect Cryosectioning Method -  Brain Blocker One™ with these features:

  • Easily reproducible alignment in atlas planes
  • Section one or multiple brains with each knife pass
  • Time saving in Tissue sectioning
  • Time saving in Microscope analysis

Brain Blocker One is a set of gel blocks, left and right half, with an internal cavity cast originally an actual mouse brain. Drop a fresh or fixed brain in to displace the liquid in the one half cavity, add another half on top and the brain is now fully encased, as if it had been embedded. The brain cavity is in sterotaxic orientation, top surface of the gel parallel to skull flat. The gel expands slowly with hydration,  and the cavity  accommodates different  brain sizes simply by the length of the presoak time,  1 to 4 hours for fresh adult brain, less for fixed or young brain, more for aged brain.

The entire block can be frozen and sectioned. The cryostat pedestal need never be readjusted from parallel to the blade plane of motion, and every brain will come out sectioned in the same plane as the atlas.

Multi-blocks can be frozen and mounted on the same pedestal together. Each pass of the cryostat knife, produces the same thickness sections, same orientation from every brain are mounted on that pedestal. Such large set of the sections can be transferred intact from the blade to the slide using our Cryo Jane Tape Transfer System (#62800-10). Up to 24 brains can be sectioned with each pass of the knife using the whole body cryostat and Macro Tape Transfer system.

Gels will come in packages of 12 pairs, slightly dehydrated. Soak in water, saline, or buffer to fit the brains

69100  Brain Blocker One™ Mouse Brain 12/pk 83.00 Add to Cart
69102 Brain Blocker One™ Rat Brain 12/pk 83.00 Add to Cart

Brain Blocker One ™  is trade mark of Mc Cormick

arrow12Sampling Tools – Harris Micro-Punch™ and Harris Uni-Cores™

The Harris Micro-Punch™ and Uni-Cores are designed to serve research scientists with a dissection tool for small pre-defined regions of the tissue or the specimen.

Harris Micro-PunchApplications

  • Forensic - Punch cut, retrieve, store and preferentially eject cored samples from source materials such as paper, gels, cloth, leaves, paint chip, films, etc.
  • Electrophysiology – Punch dissection of discrete regions prior to acute dissociation for patch recording
  • Biomedical Pharmacology – Punch removal of discrete brain regions for analysis of neurotransmitters and metabolite changes in response to different pharmacological agents.
  • Anatomy – Punch out small brain regions for analysis of neurotransmitter concentrations of mRNA levels or for subsequent use in electron microscopy.
  • Others – Positioning samples inside the quartz pyrolysis gel tubes, or on FTIR diamond cell sample stages.

Operating

For most forensic samples, position source of materials (i.e. blood cards, paint chips, paper, leaves, gels, etc.) on the self-healing cutting mat. Remove the protective cover cap from the punch. Firmly grip the punch. The tip is then positioned at a right angle above the target area – DO NOT DEPRESS THE PLUNGER. The tip is pushed downward into the source material until it makes contact with the cutting mat. Lift the Harris Micro-Punch™ away from the source material with the cored sample stored in the cutting tip.

For most biology samples – Typically tissue to be punched is first sliced into 1-2mm thick sections. (Our Tissue and Brain Matrices are specially designed for this purpose See page 198 of Catalog XIV). The section to be punched is then placed on a saline-soaked filter paper disc (EMS #70662 – Petri dish with absorbent paper) on an ice-cold dissection plate (EMS #62536-01 – Cold Plate) Then follow the above procedure.

Sample Storage and Ejection

After coring, the sample is temporarily stored in the hollow cutting tip ready for the preferred ejection. The Harris MicroPunch™ can be rested on its side without loss of sample from the hollow tip. To eject sample, position tip over target area and depress plunger.

Cleaning

Clean the tip between each sample extraction by coring blank filter paper, rinsing with ethanol or spraying with compressed air to remove dried artifacts. Rinse mat with ethanol after each sample extraction.

Harris Micro-PunchA. Harris Micro-Punch

The tips and plungers are made from high-grade 440c stainless steel. Tips are heat-treated to a Rockwell hardness of Rc-58 and then individually sharpened to a razor edge for a long cutting life. Tips are replaceable and available in sizes 0.50, 1.0, 1.20, 2.0, and 3.0 mm. The barrel and knob screw assembly are constructed from injected molded, high-tech, lubricated plastic to eliminate sticking. Length: 140mm (5 ½”). Sterilization temperature up to 180°C (360°F)

Cat # Size mm
+-0.06
HMP
Lngth
mm
I.D. mm+- 0.06 Shaft O.D.
mm
Tip Lngth mm Shft Lngth mm Shft Wall mm Edge Wall mm Lumen
Lngth
mm
Lmen Void
Vol ml
Edge
Angle
69034-05 0.5 142.04 0.50 3.20 16.8 5.50 1.30 0.001 5.40 1.060 15°
69034-10 1 142.04 1.00 3.20 16.8 5.50 1.10 0.001 6.68 5.243 19°
69034-12 1.2 142.04 1.20 3.20 16.8 5.50 1.05 0.001 5.74 6.488 23°
69034-20 2 142.04 2.00 3.20 16.8 5.50 0.65 0.001 5.88 1846 14°
69034-30 3 142.04 3.00 3.20 16.8 5.50 0.62 0.001 7.09 50.09 11°
69034-05 Harris Micro-Punch, 0.5mm each 108.00 Add to Cart
69034-10 Harris Micro-Punch, 1.0mm each 108.00 Add to Cart
69034-12 Harris Micro-Punch, 1.2mm each 108.00 Add to Cart
69034-20 Harris Micro-Punch, 2.0mm each 108.00 Add to Cart
69034-30 Harris Micro-Punch, 3.0mm each 108.00 Add to Cart
69035-05 Replacement Tip, 0.5mm each 36.50 Add to Cart
69035-10 Replacement Tip, 1.0mm each 36.50 Add to Cart
69035-12 Replacement Tip, 1.2mm each 36.50 Add to Cart
69035-20 Replacement Tip, 2.0mm each 36.50 Add to Cart
69035-30 Replacement Tip, 3.0mm each 36.50 Add to Cart
69035-06 Replacement 0.5mm Plunger each 32.00 Add to Cart
69035-07 Replacement 1.0mm Plunger each 32.00 Add to Cart
69035-08S Replacement 1.2mm Plunger each 32.00 Add to Cart
69035-08 Replacement 2.0mm Plunger each 32.00 Add to Cart
69035-09 Replacement 3.0mm Plunger each 32.00 Add to Cart

Aluminium Harris Micro-PunchB. Deluxe Aluminium Harris Micro-Punch®

Aluminum barrel is Swiss-style machined from high grade 6061 light weight, seamless, aluminium tubing and anodized to form a protective, chemically resistant long lasting surface for easy handling.

Specifications and Ordering:

Tip for Aluminium Harris Micro-Punch®

Cat # Dot size mm
+-0.06
HMP
Lngth
mm
I.D. mm+- 0.06 Shaft O.D.
mm
Tip Lngth mm Shft Lngth mm Shft Wall mm Edge Wall mm Lumen
Lngth
mm
Lmen Void
Vol ml
Edge
Angle
69033-05 0.50 141.63 0.500 3.20 16.8 5.50 1.30 0.0010 4.760 0.934 15°
69033-10 1.00 141.63 1.000 3.20 16.8 5.50 1.10 0.0010 5.740 4.506 19°
69033-12 1.20 141.63 1.200 3.20 16.8 5.50 1.05 0.0010 5.210 5.889 23°
69033-20 2.00 141.63 2.000 3.20 16.8 5.50 0.65 0.0010 4.830 15.16 14°
69033-30 3.00 141.63 3.000 3.20 16.8 5.50 0.62 0.0010 5.930 41.89 11°
69033-05 Aluminium Harris Micro-Punch, 0.5mm each 163.00 Add to Cart
69033-10 Aluminium Harris Micro-Punch, 1.0mm each 163.00 Add to Cart
69033-12 Aluminium Harris Micro-Punch, 1.2mm each 163.00 Add to Cart
69033-20 Aluminium Harris Micro-Punch, 2.0mm each 163.00 Add to Cart
69033-30 Aluminium Harris Micro-Punch, 3.0mm each 163.00 Add to Cart

Harris Uni-CoresC. Harris Uni-Cores

The Harris Uni-Core™ is a disposable multi-purpose sampling tool for soft substrates. They are made from polypropylene, including a hollow body with built-in tool steel razor sharp cutting tip, with a plunger for the expelling of the punched sample. Uni-Core comes individually wrapped and sterilized by ethylene oxide. Uni-Core can also be reusable by rinsing the tip with ethanol after each use and autoclaving for 20 minutes at 250°F/15 psi. Available sizes: 0.035, 0.5, 0.75, 1.2, 1.25, 2.0, 2.5, 3.0, 3.5, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0mm.

69036-035 Harris Uni-Core™, Size 0.35mm each 5.25 Add to Cart
69036-05 Harris Uni-Core™, Size 0.5mm each 5.25 Add to Cart
69036-07 Harris Uni-Core™, Size 0.75mm each 5.25 Add to Cart
69036-10 Harris Uni-Core™, Size 1.0mm each 5.25 Add to Cart
69036-12 Harris Uni-Core™, Size 1.2mm each 5.25 Add to Cart
69036-15 Harris Uni-Core™, Size 1.5mm each 5.25 Add to Cart
69036-20 Harris Uni-Core™, Size 2.0mm each 5.25 Add to Cart
69036-25 Harris Uni-Core™, Size 2.5mm each 5.25 Add to Cart
69036-30 Harris Uni-Core™, Size 3.0mm each 5.25 Add to Cart
69036-35 Harris Uni-Core™, Size 3.5mm each 5.25 Add to Cart
69036-40 Harris Uni-Core™, Size 4.0mm each 5.25 Add to Cart
69036-50 Harris Uni-Core™, Size 5.0mm each 5.25 Add to Cart
69036-60 Harris Uni-Core™, Size 6.0mm each 5.25 Add to Cart
69036-70 Harris Uni-Core™, Size 7.0mm each 5.25 Add to Cart
69036-80 Harris Uni-Core™, Size 8.0mm each 5.25 Add to Cart
69036-90 Harris Uni-Core™, Size 9.0mm each 5.25 Add to Cart
69036-100 Harris Uni-Core™, Size 10.0mm each 5.25 Add to Cart
69036-110 Harris Uni-Core™, Size 11.0mm each 5.25 Add to Cart
69036-120 Harris Uni-Core™, Size 12.0mm each 5.25 Add to Cart
69037D. Cutting Mats

These cutting mats are inert, self-healing and can be sterilized with ethanol. Available sizes 6 x 8” and 2 ½ x 3”

69037 Cutting Mat, Size 6 x 8” each 8.25 Add to Cart
69037-23 Cutting Mat, Size 2½ x 3” each 3.25 Add to Cart

Harris e-Core Sample Tissue CorerTissue Corer arrow12E. Harris e-Core™

Cuts, retrieves and stores cored samples in one operation

The Harris e-Core is an electric coring device created to bridge the gap between the lower cost, manual Harris Micro- Punch and Harris Uni-Core and the automated bench top punching platforms. The ergonomic “joy stick” grip and the e-Core’s simple operation provide comfort and efficiency, integrating smoothly into all levels of laboratory sample throughput. The user has complete management of the disc recovery process, the coring speed and disc delivery.

The Harris e-Core, like the Harris Micro-Punch and Harris Uni-Core, continues to offer the same unique combination of patented simultaneous sampling features: coring, retrieving and storing in a single operation, but with dramatically increased processing times.

The Harris e-Core has been designed to utilize dedicated replaceable Harris Micro-Punch Tips which thread to the Harris e-Core drive shaft. The Harris e-Core is currently available in three models .

Each of the e-Core kits contain:

  1. One Harris e-Core with tip and cover cap in place.
  2. Two extra Harris Micro-Punch tips of same size.
  3. Optional cord wrist strap.
  4. Multi-plug set includes Europe (EU), United Kingdom (UK), Australia (AUST) and North America (NA) adapters.
  5. Power supply.
  6. 6 x 8” Harris Cutting Mat.
  7. Harris e-Core Carrying Case.
  8. User Guide.
69039-12 Harris e-Core kit, 1.2mm Tip, 0.8mm Plunger (O.D.) each 580.00 Add to Cart
69039-20 Harris e-Core kit, 2.0mm Tip, 1.8mm Plunger (O.D.) each 580.00 Add to Cart
69039-30 Harris e-Core kit, 3.0mm Tip, 2.8mm Plunger (O.D.) each 580.00 Add to Cart

Sterile, Disposable Skin Biopsy Punches.arrow12Accu-Punch

The only disposable punch made in the USA!

Sterile, Disposable Skin Biopsy Punches.

Available in 14 different sizes as well as assortment packs.

  • Always Sharp, Always Sterile
  • Ergonomic Design
  • Ribbed for Maximum Control
  • Available in Dispenser boxes of 25 or 50 pieces
69038-01 Accu-Punch 1 mm 25/pk 68.00 Add to Cart
69038-01-50 Accu-Punch 1mm 50/pk 92.00 Add to Cart
69038-15 Accu-Punch 1.5 mm 25/pk 68.00 Add to Cart
69038-15-50 Accu-Punch 1.5mm 50/pk 92.00 Add to Cart
69038-02 Accu-Punch 2.0mm 25/pk 68.00 Add to Cart
69038-02-50 Accu-Punch 2.0mm 50/pk 92.00 Add to Cart
69038-25 Accu-Punch 2.5mm 25/pk 68.00 Add to Cart
69038-25-50 Accu-Punch 2.5mm 50/pk 92.00 Add to Cart
69038-03 Accu-Punch 3.0mm 25/pk 68.00 Add to Cart
69038-03-50 Accu-Punch 3.0mm 50/pk 92.00 Add to Cart
69038-35 Accu-Punch 3.5mm 25/pk 68.00 Add to Cart
69038-35-50 Accu-Punch 3.5mm 50/pk 92.00 Add to Cart
69038-04 Accu-Punch 4.0mm 25/pk 68.00 Add to Cart
69038-04-50 Accu-Punch 4.0mm 50/pk 92.00 Add to Cart
69038-45 Accu-Punch 4.5mm 25/pk 68.00 Add to Cart
69038-45-50 Accu-Punch 4.5mm 50/pk 92.00 Add to Cart
69038-05 Accu-Punch 5.0mm 25/pk 68.00 Add to Cart
69038-05-50 Accu-Punch 5.0mm 50/pk 92.00 Add to Cart
69038-06 Accu-Punch 6.0mm 25/pk 68.00 Add to Cart
69038-06-50 Accu-Punch 6.0mm 50/pk 92.00 Add to Cart
69038-07 Accu-Punch 7.0mm 25/pk 68.00 Add to Cart
69038-07-50 Accu-Punch 7.0mm 50/pk 92.00 Add to Cart
69038-08 Accu-Punch 8.0mm 25/pk 68.00 Add to Cart
69038-08-50 Accu-Punch 8.0mm  50/pk 92.00 Add to Cart
69038-10 Accu-Punch 10.0mm 25/pk 80.00 Add to Cart
69038-10-50 Accu-Punch 10.0mm 50/pk 130.00 Add to Cart
69038-12 Accu-Punch 12.0mm 25/pk 80.00 Add to Cart
69038-12-50 Accu-Punch 12.0mm 50/pk 130.00 Add to Cart
69038-AS Assorted Pack one of each size 14/pk 72.00 Add to Cart

Slide Markers, Liquid Blockers, Bio-Imager, and PAP Pens arrow12