WETSEM® Gallery
Cultured mammalian cells / Unstained
Mitochondria stained with
lead citrate in wet cells
Fully hydrated HeLa cells were fixed with 2% glutaraldehyde for 30 min and stained with 1% Venables lead-citrate for 4 min. This image shows mitochondria (~300nm in width).
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HeLa cell stained with osmium tetroxide
Fully hydrated HeLa cells were fixed with paraformaldehyde 2%/1% glutaraldehyde for 25 min and stained with osmium tetroxide 1% for 30 min. Lipid droplets appear prominently bright. Nuclei and nucleoli are also stained. In cytoplasm, staining is obtained for mitochondria and some vesicular structures.
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Wet Bovine sperm cells stained with osmium tetroxide
Fully hydrated Bovine sperm cells were fixed with 4% paraformaldehyde for 10 min and stained with 1% osmium tetroxide.
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Wet CHO cells stained with uranyl acetate
Fully hydrated CHO cells were fixed with 4% paraformaldehyde for 10 min and stained with 1% uranyl acetate.
Left: Lower magnification shows the overall staining pattern with
uranyl acetate.
Right: Structure of cell-cell contacts between neighboring cells at
higher magnification.
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Chromosomes stained with gold chloride
Cells grown in the QX-102 capsule were fixed with 2% Paraformaldehyde/1%
Glutaraldehyde/PBS for 30 min. The cells were stained with 0.1% gold chloride
for 20 min and imaged in QX-102 Imaging Buffer.
Left: NIH-3T3 cells Right:
CHO cells.
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Macrophages
Macrophages (IC21) grown in QX-102 capsules were fixed and stained with uranyl acetate. Higher magnification image (lower) shows ruffled borders of membranes. In collaboration with Paul Matsudaira (Head of MIT bio-imaging).
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Cells stained with osmium tetroxide
Cells were grown in the QX-102 capsule, fixed (2% Paraformaldehyde/1% Glutaraldehyde/PBS) and stained with osmium tetroxide.
Left: NIH-3T3 cells
Right: Hela cells. Lipid droplets in the cytoplasm are seen as bright small spheres.
Product Information
For more information on WETSEM® capsules for imaging and EDS of fully-hydrated samples with SEM, please see the Product information.
