WETSEM® Gallery

Tissues / General staining

Wet rabbit retinal pigment epithelium

Retinal pigment epithelium from a rabbit eye fixed in formalin and stained with 0.1% osmium tetroxide for 30 min. The melanosomes, the pigment-containing organelles within the epithelial villi, are seen as bright cigar-shaped structures.

Left: Overall structure of the epithelial cells, covered with the melanosomes, is seen at lower magnification. The cell nuclei are visible as the brighter spheres. Bar: 10um.

Right: A closer look at the melanosomes. Bar: 2um.

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Wet corneal endothelial Cells

Wet corneal endothelium from a rabbit eye fixed in formalin and stained for 10 minutes with 0.1% uranyl acetate.
Left: Overall structure of the convoluted endothelial cells is seen at lower magnification. Bar: 50um.
Right: higher magnification. Bar: 10um.

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Wet mucosal surface of intestines

Human epithelium fixed in formalin and stained with 0.1% uranyl acetate for 10 min.
Bars: left 100um, middle 50 um, right 20 um.

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Wet skeletal muscle

Porcine skeletal muscle fixed in formalin and stained for 10 min with 0.1% uranyl acetate. The striations are clearly visible, including internal structures, such as the Z-bands. Bar: 2um.

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Wet rabbit lens

Lens from a rabbit eye fixed in formalin and stained with 0.1% osmium tetroxide for 10 min. Bar: 5um

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Rat sciatic nerve stained with osmium tetroxide

Rat sciatic nerve was fixed with formalin and stained with 0.1% osmium tetroxide for 30 min. Cross section was imaged using the QX-302 capsule.

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Rat whole blood

Blood cells were attached to a poly-L-lysine coated QX-102 capsule membrane and fixed with 0.2% Glutaraldehyde. Cells were stained with 0.5% osmium tetroxide for 30 min

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Comparison between normal bowel and IBD (Inflammatory Bowel Disease)

Tissue samples were stained with uranyl acetate and imaged fully hydrated with QX-302 capsule. Left: Normal Bowel, Right: IBD (Crohn disease).The normal units of the epithelial layer lose their regular polygonal cell borders in the IBD. Iris Barshak et al. ""A Novel Method for 'Wet' SEM."" Ultrastructural Pathology, Volume 28, Number 1 / January-February 04.