Optimized
Labelling
Optimized Immuno Labelling using AURION BSA-c™ and AURION Blocking Solutions
Welcome
Electron Microscopy Sciences is pleased to make available the online versions of AURION newsletters. Aurion focuses on particulate labels including conventional and ultra small gold labels, and the reagents needed to make antibodies perform the way they are supposed to. In their Research and Development, Aurion deals with issues that apply to a wider research area and that may therefore be of interest to a larger part of the scientific community.
One of these issues is signal strength versus background, which is relevant to any immunodetection experiment, regardless of the marker system used. Through printed newsletters and now online, Aurion is sharing it's expertise.
The following pages attempt to explain the factors determining signal-to-noise ratios in immunohistology and immunocytochemistry as they are controlled by specimen and immunoreagent characteristics. With this knowledge in mind, it is illustrated how to obtain optimised signal-to-noise ratios by employing an appropriate blocking step and a proper incubation medium. The use of these reagents in the LM and bioassay field is supported by over a decade of successful applications in immunoelectron microscopy.
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Example from Light Microscopy
Example 1 N-Cadherin detection in heart muscle cells
Immunofluorescence using Alexa 568 labeled Fab goat antimouse. For reasons of comparability areas of specific labelling are pictured with similar density (arrowheads).
Left hand panel: Background using a commonly used protocol obscures sites of specific labelling.
Right hand panel: N-Cadherin immunolabelled areas obtained using Aurion Blocking Solution and BSA-c™ stand out with much clearer definition.
Courtesy of Lauren Hruby and John Harris, Dept. Physiology, University of Otago, Dunedin, New Zealand
