Bismuth Stain Kit for Specific En Bloc Staining

EMS #11430

Bismuth Stain Kits for Specific En Bloc Staining Kit consists of:

• 10x5ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)
• 10x10ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0)

Procedures

Staining solution:

Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.

Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Postfixation is carried out with 1% Osmium Tetroxide in 0.05M Sodium Cacodylate buffer, pH 7.2.

Post-staining is not needed.

Reference

Locke & Huie, (1977). Bismuth staining for Light and Electron Microscopy. Tissue Cell 9, 347.

Online Ordering

Bismuth Stain Kits for Specific En Bloc Staining is available online from the EMS Catalog. For ordering or product information, click here.