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Technical Data Sheets

Technovit H7100 / H8100 Giemsa Stain

EMS Catalog # 14653-14654

Giemsa Stain For Glycol Methacrylate Sections

This Giemsa stain works well for a variety of tissues.

Procedure

1. Stain in Giemsa working solution at room temperature for 1½ hours.
2. Rinse in dilute acetic acid (four drops in 100ml DI water) for two seconds.
3. Dip in 96% alcohol.
4. Dip in 96% alcohol.
5. Rinse in isopropyl alcohol three times for two minutes each.
6. Clear in xylene and mount.

Results

Solutions

Giemsa Solution:

• 0.15g Azure A
• 0.30g Methylene Blue
• 0.36g Eosin Y
• 0.04g Phloxine B
• 50ml Glycerine
• 40ml Methanol
• Add dyes to Glycerine and methanol, mix well. Incubate overnight in 55-58°C oven stirring occasionally. Stable for year.

Giemsa Working Solution:

• 4ml Giemsa stock solution
• 40ml phosphate buffer solution

Phosphate Buffer Solution:

• 7.25g Sodium phosphate, monobasic
• 2.75g Sodium phosphate, dibasic
• 1000ml Distilled water
• 10ml 10% Triton X-100
• Store at 4°C.

Online Ordering

Technovit Glycol Methacylate Kits H7100 / H8100 are available online from the EMS Catalog. For ordering or product information, click here.