Technovit
H7100 / H8100 Hematoxylin And Eosin Stain
EMS Catalog #14653-14654
Hematoxylin And Eosin Stain For Glycol Methacrylate Sections
Hematoxylin and Eosin stain is a good general stain for many types of tissue.
Solutions
Gills Hematoxylin
- 6g Hematoxylin (C.I. 75290)
- 0.6g Sodium Iodate
- 52.8g Aluminum Sulfate
- 690ml Distilled Water
- 250ml Ethylene glycol
- 60ml Glacial acetic Acid
Eosin
- 0.5g Eosin Y (Alcoholic C.I. 45380)
- 100ml Ethanol 96%
- 2 drops Glacial Acetic Acid
Acid Ethanol
- 4ml 0f 25% HCl
- 100ml of 70% Ethanol
Procedure
- Stain in Gill's Hematoxylin for 15 minutes.
- Wash in tap water for 10 minutes.
- Decolorize plastic, if necessary, with acid alcohol followed by tap water rinse.
- Rinse in distilled water.
- Scott's Tap Water for one to two minutes.
- Two changes of distilled water.
- Counterstain with Eosin for two to five minutes.
- Dehydrate using 96% and 100% ethanol.
- Clear in xylene and cover slip.
Results
| Nuclei | blue |
| Basophilic cytoplasm | blue |
| Acidophilic cytoplasm | pink |
| Muscle tissue | pink |
| Connective tissue | pink |
Online Ordering
Technovit Glycol Methacylate Kits H7100 / H8100 are available online from the EMS Catalog. For ordering or product information, click here.