Technovit
H7100 / H8100 Periodic Acid Schiff Stain
EMS Catalog #14653-14654
Periodic Acid Schiff Stain For Glycol Methacrylate Sections
Procedure
- Oxidize sections in 0.4% periodic acid for 30 minutes at 57°C.
- Wash in running tap water or sodium sulfite solution (to avoid a general pink background).
- Rinse in three times in distilled water.
- Schiff's Reagent for 15 minutes.
- Wash well in running tap water.
- Rinse in distilled water.
- Counterstain with Gill's Hematoxylin for 10 minutes.
- Wash well in running tap water.
- Dehydrate using 95% and 100% ethanol.
- Clear in xylene and cover slip.
Results
| Nuclei | blue |
| Glycogen | violet / red |
| Basement membranes | violet / red |
| Mucine | violet / red |
Solutions
Gill's Hematoxylin:
- Hematoxylin (C.I. 75290) - 6 gm
- Sodium Iodate - 0.6gm
- Aluminum Sulfate - 52.8 gm
- Distilled water - 690 ml
- Ethylene Glycol - 250ml
- Glacial acetic acid - 60ml
Schiff's Reagent :
- Mix 0.5gm of Pararosaniline (C.I.#42500) into 15ml of 1N Hydrochloric Acid.
- Mix 0.5gms of Potassium Metabisulfite into 85ml of distilled water.
- Add the second solution to the first and store for 24 hours in a dark place.
- Bleach the resulting solution with 200mg of bone charcoal and filter.
- Store the colorless solution at 4°C.
Sodium Sulfite Solution:
- 0.5gm of sodium sulfite
- 100ml of distilled water
Online Ordering
Technovit Glycol Methacylate Kits H7100 / H8100 are available online from the EMS Catalog. For ordering or product information, click here.