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Technical Data Sheets

Limonene Mounting Medium

EMS Catalog #17987

Description:

This mounting medium is made with limonene a natural product from orange peels.  It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in Immunohistochemistry (IHC), e.g. DAB and DAB with nickel or cobalt.  This Organo™ Mounting Medium is also suitable with alkaline phosphatase chromogens, an organic solvent resistant Super Fast Red (IBSC). It is an excellent choice for mounting H and E stained slides.

Intended Use:

Mounting tissues and cell smears in Immunohistochemistry and mount H & E stained slides.

Reagent:

Ready to use mounting medium

Storage:

Room temperature. Do Not Refrigerate Or Freeze.

Procedure:

1. Dehydrate slides with dehydrating agents, e.g. 30 to 100% alcohol followed by Xylene, toluene or any other dehydrating reagents.
2. Add 2-3 drops of Limonene Mounting Medium.  Apply coverslip carefully, avoiding air bubbles.
3. Visualize slide under a microscope.  The slides can be dried by leaving at room temperature overnight or by heating at 37-40°C for several hours.

Online Ordering

Limonene Mounting Medium is available online from the EMS Catalog. For ordering or product information, click here.