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Technical Data Sheets

Immuno In-Situ Mounting Medium™

EMS Catalog #17988

Description:

Immuno in Situ mounting medium is a unique mounting medium designed for permanent preservation of tissue sections and cell smears with alkaline phosphatase chromogens like NBT/BCIP and Red counter stain.

Intended Use:

Mounting tissues and cell smears.

Reagent:

Ready to use mounting medium

Storage:

Room Temperature

Procedure:

1. Rinse slide to be mounted with Distilled or De-ionized Water, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
2. Turn the vial upside and remove any air bubbles.
3. Apply 3-4 drops directly on the top of specimen and spread out evenly by tilting back and forth or spread evenly with a 0.2ml plastic pipette tip.
4. Let stand at room temperature for about 60-90 minutes or heat slides at 37-40°C for 40-50 minutes. A Coverslip Is Not Required.
5. The specimen is ready for visualization under a microscope.

There are two ways to look at slide under oil immersion lens.

1. For oil immersion lens, a drop of oil can be placed directly on dried Immuno in Situ mounting medium for visualization. The oil can be removed by wiping with Kimwipes.
2. When the mounting medium has completely dried, apply 1-2 drops of any organic mounting medium on top of dried mounting medium; which has protected the specimen, cover slide with coverslip; the slide is ready for visualization.

Removal Of Mounting Medium:

If coverslip is not applied, the mounting medium can be removed by soaking slide in warm (37°C) water for 10-20 minutes. Rinse the slide several times with warm water to remove the entire mounting medium. The slide can be remounted again.

Online Ordering

Immuno In-Situ Mounting Medium™ is available online from the EMS Catalog. For ordering or product information, click here.