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Electron Microscopy Sciences

Technical Data Sheets

Truant's Fluorescent Method For Acid Fast Organisms

EMS Catalog #26102


10% Buffered Neutral Formalin


Paraffin, @ 6 microns

Staining Procedure

Use control slide
  1. Deparaffinize and hydrate to distilled water.
  2. Weigert's Iron Hematoxylin solution for 10 minutes. Prepare working Weigert's Hematoxylin by mixing 1 vol. of Weigert's Hematoxylin A, with 1 vol. of Weigert's Hematoxylin B. Wash in running water for 10 minutes.
  3. Auramine-Rhodamine Solution, @ 60 °C for 10 minutes, or 37 °C for 15 minutes, or 25 °C for 20 minutes. Wash in tap water.
  4. Remove excess stain with Acid Alcohol, 1%, for 2-3 minutes. Wash in running water. Rinse in distilled water.
  5. Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene, two changes each.
  6. Mount with Permount.


Acid Fast flourescent reddish yellow
Artifacts flourescent yellow


Traunt, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962.

AFIP Manual of Histological Staining Methods, 3 rd ed. , Ed. L. Luna: New York : McGraw Hill
Publications, c. 1968, p. 219.

Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore , 3 rd Ed., c. 1973, p. 225.

Online Ordering

Weigert’s Iron Hematoxylin Solution A, Weigert’s Iron Hematoxylin Solution B, Auramine-Rhodamine Staining Solution and Acid Alcohol 1% for Truant's Fluorescent Method for Acid Fast Organisms are available online from the EMS Catalog. For ordering or product information, click here.