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EMS Technical Data Sheets
Wright-Giemsa Stain Method
EMS Catalog #: 26149-Series
Fixation:
Streak thin (approx. one cell thick) smears across a sterile slide by means of a second slide or cover glass. Air-dry quickly.
Staining:
- Place 1.0 ml of the Wright-Giemsa Stain (#26149-01) upon the smear, in sufficient quantity to cover the entire surface, for 3-4 minutes.
- Add 2.0 ml distilled water or Phosphate Buffer, pH 6.5 (#26149-02) and let stand twice as long as in step 1.
- Rinse stained smear with water or the Phosphate Buffer, pH 6.5 (#26149-02) until the edges show faintly pinkish red.
- The film is allowed to dry in the air. The preparation may be blotted gently to hasten drying.
- Permanent mounts can be made with mounting medium.
Stain Results:
| Erythrocytes | Pink-Tan |
| Eosinophiles | Red |
| Lymphocytes: Granules | Red-Purple |
| Lymphocytes: Cystoplasm | Blue |
| Neutrophils: Granules | Purple-Pink |
References:
Lillie, R.D.: Factors influencing the staining of blood films and the role of methylene violet. J. Lab. Clin. Med. 29: 1181, 1944
Lillie, R.D., H.J. Conn’s Biological Stains, 8th edition, William’s & Wilkins, Baltimore, p. 438, 1972.