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Technical Data Sheets

Maximow's Method for Bone Marrow

EMS Catalog #: 26252-Series

Fixation:

Zenker’s / Formalin

Sections:

Paraffin at 6 microns

Staining Procedures:              

1. Deparaffinize and hydrate to distilled water.
2. Mayer’s Hematoxylin (#26252-01) for 15 minutes. Wash in tepid tap water for 15 minutes.
3. Stain in working solution overnight. Prepare working solution fresh each time as follows:
   1. Eosin Y, 0.1%, Aqueous (#26252-02) - 10 ml
      Triple distilled water or Phosphate Buffer (#26252-04) - 100 ml
      Azur II, 0.1%, Aqueous (#26252-03) - 10 ml
      Glacial Acetic Acid - 2 drops
4. Differentiate in 95% alcohol until blue ceases to come out into alcohol and erythrocytes and collagen are pink.
5. Dehydrate in absolute alcohol, clear in xylene, two changes each.
6. Mount with Permount (#17986-01).

Stain Results:

References:

AFIP, Manual of Histologic Staining Methods, 3rd edition, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120

Mallory, F.B., Pathological Technique, New York, Hafner Publishing Co., 1961, p. 196.

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