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Technical Data Sheets

Lillie Modification of Mason's Trchrome Stain

EMS Catalog #: 26364-Series    

Fixation:

Bouin’s (#15990-01) or Russell’s Zenker -contain no Mercury (#64123-05).

Sections:

Paraffin at 6µm.

Staining Procedures:

1. Deparaffinize and hydrate to distilled water.
2. If Zenker’s contain Mercury is used as fixative, remove Mercuric Chloride Crystal by placing in Lugol’s Iodine (#26364-07) or Gram’s Iodine (#26364-7A) for 15 minutes. Rinse in water and clear in Sodium Thiosulfate, 5% (#26364-08) for 3 minutes. Wash in water for 10 minutes or longer. If Russell’s Zenker (#64123-05) is used, go to step #3.
3. Stain in Weigert’s Iron Hematoxylin Working* solution for 5 minutes. Wash in running water.
   1. * To prepare working solution, mix equal parts of Weigert’s Iron Hematoxylin A (#26364-02) and Weigert’s Iron Hematoxylin B (#26364-03).
4. Stain in Biebrich Scarlet Solution (#26364-04) for 4 minutes. Rinse in water
5. Mordant in Phosphomolybdic Acid-Phosphotungstic Acid (#26364-01) for 1 minute.
6. Stain in Fast Green FCF Solution (#26364-05) for 4 minutes.
7. Differentiate in Acetic Acid, 1% (#26364-06) for 1 minute.
8. Carry through alcohol and dehydrate with acetone or absolute alcohol.
9. Clear and mount in balsam saturated with salicylic acid.

Stain Results

References:

Clark, G.: Staining Procedures. Williams and Wilkins Co., Baltimore, 3rd ed., c. 1973, p. 59

Lillie, R.D., Arch. Path., 29:207, 1940.

Online Ordering

Lillie Modification of Mason's Trchrome Stain is available online from the EMS Catalog. For ordering or product information, click here.