EMS Technical Data Sheets

Thermanox® Coverslips

EMS Catalog #72274

Purpose

Cell attachment and growth is equal to or better than polystyrene plastic or glass.

Warning

Suggestions for use

Note: Thermanox coverslips may float due to air bubbles or surface tension. Air bubbles may form when the coverslip is placed in the medium, or if the medium is below incubator temperature. Bubbles may form as the medium comes up to the temperature releasing dissolved gas.

Coverslips may be pushed to the bottom of a dish or other container with sterile forceps, pipette or gentle agitation. In Leighton tubes, a gentle shake will cause the coverslip to settle to the bottom of the vessel.

To prepare semi-permanent/permanent preparations for light microscopy

  1. Fix cells grown on Thermanox coverslips in methanol, acetone or other fixative.
  2. Stain.
  3. Dehydrate in acetone.
  4. Clean in Xylene.
  5. Cover with a mountant.
  6. Place clear glass coverslip on top.

Examine the cells through the glass coverslip and mountant for improved resolution.

To Prepare for Transmission Electron Microscopy

  1. Fix cells with routine fixative (Glutaraldehyde).
  2. Post fix with OsO4 . Rinse and prestain.
  3. Dehydrate in ethanol followed by propylene oxide.
  4. Infiltrate with Epon.
  5. Invert on previously polymerized Epon Blank, wipe back and polymerize
  6. Remove coverslip by inverting on a warm hotplate and peeling off or by touching coverslip to dry ice. The coverslip will peel off leaving the cells on the Epon block.

References

  1. Buckley, C.E. Coverslips for Use in Tissue Culture. Laboratory Equipment Digest, May, 1976
  2. Deter, R.L. Quantitative Morphological Analysis of Early Mouse Embryogenesis In Vitro. Perfusion Culture System. Tissue Preparation: Sampling. Journal Embryol. Exp. Morph. 40:19-100. 1977
  3. Pauli, B.U., Anderson , S.N., Mernoli, V.A. and Kuettner K.A. Development in an In Vitro and In Vivo Epithelial Tumor Model for the Study of Invasion. Cancer Research 40: 4571-4580. December, 1980
  4. Werb, Z., Bainton, D.F., and Jones, P.A. Degradation of Connective Tissue Matrices by Macrophages. Journal of Experimental Medicine, 152: 1537-1553. December, 1980
  5. Swierenga, S.H.H., Whitfield, J.F., and Morris, H.P. The Reproduced Extracellular Calcium Requeirement for Proliferation by Neoplastic Hepatocytes. In Vitro. 14 No 527-535. 1978
  6. Zembala. M., Lemmel, E.M., Uracz, W. Activation of Human Monocytes for Nitroblue Tetrazzolium Reduction and the Suppression of Lymphocytes Response to Mitogens. Clin.Exp. Immunol. 41: 309-316: 1980

Online Ordering

Thermanox® Coverslips are available online from the EMS Catalog. For ordering or product information, click here.