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Electron Microscopy Sciences

Technical Data Sheets

SampleSaver™

EMS Catalog #76540-01, 76540-02

Directions for Use

SampleSaver
Step 2
SampleSaver
Step 5
SampleSaver
Step 7
  1. Attach a ¼" OD flexible tube for the gas supply into the valve inlet. Use an inert gas such as Ar, N2, CO2, etc. that will be used to preserve the sample. The delivery pressure of the gas should be no greater than about 5 psi and should be able to be decreased to a very low flow just before closing. The pressure when the unit is closed should be no greater than 1 psi.
    Warning: Please set the regulator pressure and make sure gas is flowing at a low flow rate prior to attaching tubing to the SampleSaver™ container. If the container is exposed to a high pressure, it could explode and cause injury. It is preferable to have the gas flowing and the container in the purging configuration prior to putting the tubing onto the valve.
  2. After inserting samples into the SampleSaver™ container, screw the lower portion of the container into the upper portion, but stop when the lip of the lower portion is just below the hole in the upper portion as shown by the red arrow in photo "Step 2".
  3. Allow the gas to flow. If the gas flow is variable, use a high flow rate initially. The longer the gas is allowed to flow, the more complete is the replacement of the air in the container by the inert gas. The amount of time depends on the delivery flow of the gas.
  4. When the volume has been purged, decrease the flow to a low value. One way to gauge the flow is to wet your lips and allow the gas escaping the body to just give a cooling sensation. Do not allow the gas to stop flowing.
  5. Stop the flow by further turning the lower portion of the SampleSaver™ container until the body O-ring is just above the hole in the upper portion as shown by the red arrow in photo "Step 5".
  6. Close the supply valve.
  7. Compress the body of the SampleSaver™ container, by continuing to screw the lower portion of the container into the upper portion until it stops as shown red arrow in photo "Step 7".

Checking and Releasing

  1. To check the SampleSaver™ storage container to see whether the inert atmosphere is still present, open the valve while the body is still compressed. A sound of released gas indicates that the gas was still present. If it is still desirable to keep the inert gas intact, but only to check whether the container is still pressurized, insert one end of ¼" tubing into a liquid nitrogen (LN2) container (such as a Styrofoam cup with LN2) and while the N2 is spurting out the other end, attach that end to the valve. When the liquid nitrogen has calmed down from the insertion of the tube, open the valve. If there is a bubbling from the tube into the LN2, then the gas was still under pressure. In this case, the pressure inside the container is now at atmosphere, but the nitrogen is still preserving the sample. Do not remove the tube from the LN2.
  2. To repressurize container after checking it, simply unscrew the bottom portion from the top portion of the SampleSaver™ container until the body O-ring is just above the hole in the upper portion as shown by the arrow in photo "Step 5". Close the valve and compress the body of the SampleSaver™ container, by continuing to screw the lower portion of the container into the upper portion until it stops as shown in photo "Step 7". The container if now repressurized.
  3. If it is desired to remove samples, simply unscrew the lower portion of the container and separate the container.

Maintenance

The only maintenance that the SampleSaver™ storage container should require is periodic lubrication of the body O-ring. Fomblin® perfluoropolyether pump oil was used in the assembly.

Product Information

SampleSaver™