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Electron Microscopy Sciences

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Keyence VHX Fluorescence Images

The NIGHTSEA Model SFA adapter system adds a versatile fluorescence capability to the Keyence VHX Digital Microscope series, and the combination is being used in an ever-expanding variety of applications. The adapter works with many of the available Keyence lenses, including the ZST, Z100, Z50, Z20, and Z00. Visit our Keyence product page for more information.

The images below are a sampling of those we have made during our own development and testing or in collaboration with customers.

Electronic component failure analysis

Examining electronic components that were embedded in epoxy, cross-sectioned, polished, and highlighted with a fluorescent epoxy-like dye. Images were made with the Keyence VH-ZST lens. While the manufacturer of the fluorescent dye recommended excitation with ultraviolet light, our Royal Blue light head was used for these images, and was found to be superior to ultraviolet.

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Circuit board conformal coating

Conformal coating on a circuit board, with damage. Images made with Keyence VH-ZST lens. The upper row was made with Ultraviolet light, the 'traditional' wavelength range for this application. While this works well with the excitation directed from the side, the ultraviolet wavelengths do not work well with the ZST lens when the light head is positioned over the fiber optic input port. The lower row shows the same areas under excitation with our Violet light head. While the fluorescence is marginally less bright at lower magnifications, this source works better through the Keyence lens optics, producing the brighter area you can see in the 200x image.

Ultraviolet excitation

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Violet excitation

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Concrete thin section

The images below were made from a 20µm concrete thin section with the Keyence VH-Z100 lens, NIGHTSEA Royal Blue excitation. As with the electronic components above, the manufacturer of the fluorescent dye recommended excitation with ultraviolet light. Our Royal Blue light head was used for these images and was found to be superior to ultraviolet.

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Thin section courtesy of Department of Mineralogy and Geochemistry, Institute of Geoscience and Geography, University Halle-Wittenberg Germany.

Epoxy on a motor shaft

Small motor shaft with epoxy where it does not belong. Imaged with Keyence VH-ZST lens, 20x magnification.

  • Epoxy on motor shaft, 20x

Gel defect in a nylon granule

Images made with Keyence VH-ZST lens, Royal Blue excitation. At 100x and above, the images utilized the Keyence automated focus stacking capability.

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Fluorescent-dosed plastic particles

Fluorescent-dosed plastic particles, 38-45 microns. Image made with ultraviolet excitation. Image courtesy of an explosives detection company.

  • Fluorescent-dosed plastic particles, 38-45 microns. Image made with ultraviolet excitation. Image courtesy of an explosives detection company.

Naturally fluorescent mineral

This mineral sample, collected at the Sterling Hill Mine in Ogdensburg, New Jersey, contains willemite (green fluorescence), calcite (red fluorescence) and franklinite (black – no fluorescence).

  • Willemite, 20x
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  • Willemite, composite 20x and 1000x
  • Fluorescent mineral – Willemite, 2000x
  • Willemite, 1000x

Ammonite fossil

Sectioned and polished fossil of an ammonite, an early (and now extinct) cephalopod, related to modern-day nautilus, squid, cuttlefish, and octopus.

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Ammonite fossil fluorescing under the Keyence microscope, VH-ZST lens, Royal Blue excitation.

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