Workshop Information
Please view our schedule to see if this course is currently offered.
Microscopy: The Complete Image
Do you want to add to your skills in Scanning, Transmission, Optical Light, or General Microscopy?
Are you working in a lab that requires you to be proficient in all fields of microscopy?
If the answer is yes to either of those questions, then this is the class for you!
Walk away in two short weeks with the ability to run and be proficient in all aspects of specimen preparation and biological materials.
Be a glowing asset in any lab you walk into!
Examples of the endless possibilities in the field of Microscopy
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Targeted Participants
Individuals who are currently employed in the biological field and want to add a significant skill level to their resume. Also anyone with an undergraduate degree in the sciences and need a specific skill set for employment. Limit: 4 - 6 students.
Scope of Class
This 2-week intensive course will prepare participants to enter the field of microscopy with a strong emphasis on electron microscopy specifically. Both in-depth theory and extensive hands-on lab work in specimen preparation methodologies, instrumentation, and image assessment are the prime focus of this course.
Sample preparation will cover cell/tissue requirements, tonicity, buffers, fixatives evaluation, fixation, dehydration and embedment. The process of microtomy, 0.5 μm and 60 nm, for OLM and TEM respectively, will take up a significant portion of the hands-on lab exercises. The theory and practical operation of the OLM, SEM, and TEM will complete the course and give the participant the information and skills necessary to function as an electron microscopy technician.
Facility
The EMS Microscopy Academy
Located in Hatfield, Pennsylvania, the Academy provides electron microscopy classes, workshops and training sessions for all fields of microscopy, including materials science and biological science.
Format
Lecture, demonstration and lots of personal hands-on training and application of electron microscopy techniques.
Main Curriculum
- Optical Light
- Biological Sample Preparation
- Transmission Electron Microscope
- Scanning Electron Microscope
Instruments Available
| OLM | - Reichert-Jung Polyvar - Jenco |
| SEM | - Hitachi S-3500 - COXEM SEM EM-30N |
| TEM | - Zeiss 10C |
| Ultramicrotome | - Leica EM UC7 (RT & Cryo) |
| Sample Processing | - Lynx II automated tissue processor - EMS 9000 microwave |
Faculty
Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than 35 years in EM technical education and research experience. He has been training EM students for 29 years and has developed curricula and lab exercises for TEM, SEM, OLM, lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, Dow Chemicals, Io Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Innovations, and in the process gained insight to the various applications of EM.
Al Coritz
Al has been working in the Electron Microscopy field for 38 years, beginning at the Yale School of Medicine and ending up on the commercial side with several key EM companies. His specialty is Cryo-techniques and Thin Film Technology: i.e. Freeze Fracture/Rotary Shadowing, High Pressure Freezing, and more. He is currently with Electron Microscopy Sciences where he has been the Technical Director for over 20 years.
