Automated and Rapid Specimen Processing for Electron Microscopy Workshop: A Complete Picture
This class will introduce participants to the use of equipment for processing tissue samples for TEM. 
Examples of the endless possibilities in the field of Microscopy
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| Eight mPrep/s capsules containing glutaraldehyde-fixed kidney specimens were mounted on the ASP-1000 before initiating the 45-minute processing program. Rapid processing, achieved by fluid exchanges occurring every half second, constantly delivered fresh reagent to the specimens. -Microscopy Innovations Application Note #601 |
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| Cascade Hops Leaf -Michael Kostrna, MATC |
Neuron Stem Cells -Michael Kostrna, MATC |
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Targeted Participants
Lab managers or technicians who are responsible for the processing of samples for TEM analysis and currently do not incorporate either a microwave or automated processor in their lab.
Facility
The EMS Microscopy Academy
Located in Hatfield, Pennsylvania, the Academy provides electron microscopy classes, workshops and training sessions for all fields of microscopy, including materials science and biological science.
Details
Monday, September 11, 2017, 8:00 a.m. - 5:00 p.m. to
Tuesday,
September 12, 2017, 8:00 a.m. - 1:00 p.m.
Hatfield, Pennsylvania, USA
Scope of Class
The preparation of samples for EM requires many steps, with extended wait times in between, requiring an entire day of a technician’s time. This process consists of several fixative, wash, dehydration, and resin infiltration steps, which are tedious and prone to temporal variability between runs.
With the use of an automated tissue processor or microwave, a technician’s time is reduced and better continuity between processing runs is obtained.
This class will introduce participants to the use of a dedicated lab microwave, EMS 9000, and 2 models of automated tissue processors Lynx II, and Microscopy Innovation’s ASP 1000 for processing tissue samples for TEM.
Format
Lecture, demonstration, and hands on operation of systems used. Discussions about advantages and disadvantages, along with participant questions, are a significant component of this workshop.
Main Curriculum
- EM sample preparation requirements / time table
- Microwave theory and processing
- Lynx II setup and operation
- Microscopy Innovations ASP 1000 setup and operation
Instruments Available
| EMS 9000 Microwave | Lynx II Processor | Microscopy Innovations ASP 1000 |
Faculty
Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than 35 years in EM technical education and research experience. He holds and A.S. in Biological Electron Microscopy from San Joaquin Delta College and an M.A in Education in Math, Science, and Technology. He has been training EM students for 29 years and has developed curricula and lab exercises for TEM, SEM, OLM lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, and Dow Chemicals, to Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Innovations, and in the process gained insight to the various applications of EM in industry.
Al Coritz
Al has been the Technical Director for Eelctron Microscopy Sciences for over 20 years. His previous experiences at Yale School of Medicine, Balzers, and RMC provides him with a broad depth of experience in biological EM, instrumentation, and especially cryo applications.
Schedule
Monday, September 11, 2017| 8:00-8:30 | Introduction of staff and participants | |
| 8:30-9:30 | Setup and initiate processing run on Lynx II | |
| 9:30-10:00 | Bench top processing schedule | |
| 10:00-10:15 | Coffee break | |
| 10:15-12:00 | Microwave theory and automatic processor discussion | |
| 12:00-12:30 | Hosted lunch | |
| 12:30-1:30 | Setup and initiate processing run on ASP 1000 | |
| 1:30-4:30 | Microwave processing run | |
| 4:30-5:00 | Resin embedment from 3 processing runs, polymerize overnight | |
Tuesday, September 12, 2017
| 8:00-8:30 | Discussion of previous day |
| 8:30-9:00 | Block assessment of different processing runs |
| 9:00-9:30 | Round table discussion of various processing methods |
| 9:30-12:00 | Trimming, thick sectioning and staining for OLM tissue assessment |
| 12:00-12:30 | Hosted lunch |
| 12:30-1:00 | Closing |
Lodging
Participants are responsible for making their own hotel reservations. The following hotel has been designated as the host hotel:
Homewood Suites
1200 Pennbrook Parkway
Lansdale, PA 19446
Phone: 215-362-6400
The special rates are $119.00 per night (plus tax) which includes a hot breakfast and a light dinner in the evening.
Please make your reservations and mention you are participating in the EMS Workshop.
GROUP CODE: EMS WORKSHOP
Everyone should plan to arrive the evening of September 10th.
Enrollment Note
Registration will be limited to a maximum of 15 participants.
EMS will provide samples to those who prefer not to bring their own.
Registration Fee $495.00
Includes
A workshop syllabus, all supplies, reagents and solutions, lunches, coffee, tea.Online Registration
Add your registration to your cart using the links below to use our secure checkout and pay by credit card.
During checkout please indicate:
- The name of the course
- Will you bring your own specimens? Yes / No
- What samples you are bringing and are most interested in?
If only registering for a class, select any method of shipment to proceed, as shipping charges will not apply.
If ordering products in addition to registration, select desired shipping method.
| Regular Tuition Registration: | $495.00 | |
Pay by Check
Make payable to EMS and reference "Rapid Processing Workshop".
Return your registration to:
Stacie Kirsch
1560 Industry Road
Hatfield, PA 19440 USA
Please contact Stacie Kirsch for more information.
Phone: 215-412-8400 Fax: 215-412-8450
E-Mail: [email protected]
