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Electron Microscopy Sciences

EMS Microscopy Academy Applications

arrow13Microscopy: The Complete Image

Do you want to add to your skills in Scanning, Transmission, Optical Light, or General Microscopy?

Are you working in a lab that requires you to be proficient in all fields of microscopy?

If the answer is yes to either of those questions, then these are the classes for you! Walk away in three short weeks with the ability to run and be proficient in all aspects of specimen preparation and biological materials.

Be a glowing asset in any lab you walk into! We are offering three options this summer! Sign Up

Examples of the endless possibilities in the field of Microscopy

 
 
 

Details

Three 3-Week Comprehensive Microscopy Summer Courses

June Course

Monday - Friday
June 11 - 29, 2018
8:30 a.m. - 5:00 p.m.
Hatfield, Pennsylvania, USA

July Course

Monday - Friday
July 9 - 27, 2018
8:30 a.m. - 5:00 p.m.
Hatfield, Pennsylvania, USA

August Course

Monday - Friday
August 6 - 24, 2018
8:30 a.m. - 5:00 p.m.
Hatfield, Pennsylvania, USA

Targeted Participants

Individuals who are currently employed in the biological field and want to add a significant skill level to their resume. Also anyone with an undergraduate degree in the sciences and need a specific skill set for employment. Limit: 4 - 6 students.

Scope of Class

This 3-week intensive course will prepare participants to enter the field of microscopy with a strong emphasis on electron microscopy specifically. Both in-depth theory and extensive hands-on lab work in specimen preparation methodologies, instrumentation, and image assessment are the prime focus of this course.

Sample preparation will cover cell/tissue requirements, tonicity, buffers, fixatives evaluation, fixation, dehydration and embedment. The process of microtomy, 0.5 µm and 60 nm, for OLM and TEM respectively, will take up a significant portion of the hands-on lab exercises. The theory and practical operation of the OLM, SEM, and TEM will complete the course and give the participant the information and skills necessary to function as an electron microscopy technician.

Facility

The EMS Microscopy Academy
Located in Hatfield, PA, the Academy provides electron microscopy classes, workshops and training sessions for all fields of microscopy, including materials science and biological science.

Format

Lecture, demonstration and lots of personal hands-on training and application of electron microscopy techniques.

Main Curriculum

  • Optical Light Microscope
  • Biological Sample Preparation
  • Transmission Electron Microscope
  • Scanning Electron Microscope

Instruments Available

OLM - Reichert-Jung Polyvar
- Jenco
SEM - Hitachi S-3500
TEM - Zeiss 10C
Ultramicrotome - Leica EM UC7 (RT & Cryo)
- Boeckeler RMC PowerTome (RT & Cryo)
Sample Processing - Lynx II automated tissue processor
- EMS 9000 microwave

Faculty

Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than 35 years in EM technical education and research experience. He has been training EM students for 30 years and has developed curricula and lab exercises for TEM, SEM, OLM, lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, Dow Chemicals, Io Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Innovations, and in the process gained insight to the various applications of EM.

Al Coritz
Al has been the working in the Electron Microscopy field for 39 years, beginning at the Yale School of Medicine and ending up on the commercial side with several key EM companies. His specialty is Cryo-techniques and Thin Film Technology: i.e. Freeze Fracture/Rotary Shadowing, High Pressure Freezing, and more. He is currently with Electron Microscopy Sciences where he has been the Technical Director for over 20 years.

Schedule

Week 1

  • Facility tour and organization of program
  • Optical Light Microscope: Nature and qualities of light, optics, parts, alignment
  • Microtomy: Instrument parts/function, trimming, thick sectioning, thin sectioning
  • Section Staining: Chromatic (OLM), heavy metal (TEM)
  • Biological Sample Preparation for OLM and TEM: Lynx II, EMS 9000
  • Nature of Biological Specimens
  • Solutions Math: Molar (g= M x GFM x Vol (L)), % (V/M = % x Vol), C1 x V1 = C2 x V2
  • Processing requirements: Buffers (tonicity, pH), fixatives, dehydrants, resins
  • TEM: Optics, illumination, imaging, column alignment, image focus & collection, image formation, objective astigmatism correction

Week 2

  • Vacuum: Theory, unit conversions, pumps, gauges
  • SEM: Theory, beam specimen interactions, signal collections, operational parameters, alignments, specimen preparation, mounting, sputter coating, SEM operation
  • Cell ultrastructure, cytoskeleton, junctions, surface specializations, organ histology
  • Continued Lab Work: Microtomy, OLM, TEM operation

Week 3

  • Special Applications: OLM (fluorescence, phase contrast, DIC), SEM (variable pressure, cryogenic, x-ray microanalysis), TEM (darkfield, diffraction)
  • Refinement of Skills: Tissue processing, microtomy, OLM, TEM, SEM operation
  • Final Project: correlative microscopy OLM, TEM, SEM of biological sample

Lodging

Participants are responsible for making their own hotel reservations. The following hotel has been designated as the host hotel:

Homewood Suites
GROUP CODE: EMS WORKSHOP
1200 Pennbrook Parkway
Lansdale, PA 19446
Phone: 215-362-6400

The special rates are being negotiated and includes a hot breakfast and a light dinner in the evening. Lunch will be provided at the Academy.

Please make your reservations and mention you are participating in the EMS Summer Workshop.
Everyone should plan to arrive the night before class begins.

Travel

The closest airport to Hatfield, PA is the Philadelphia International Airport. Distance from the airport to the hotel is 30 miles; drive time is approximately 35 minutes, depending on traffic.

Click here for a link to the hotel and directions.

Transportation will be provided from the hotel to the Academy and back.

Registration Fee $2,950.00

Includes

A workshop syllabus, all supplies, reagents and solutions, lunches, coffee, tea, and dinner on the opening evening of the course.

Online Registration

Add your registration to your cart using the links below to use our secure checkout and pay by credit card.

During checkout please indicate:

  • The name of the course
  • Will you bring your own specimens? Yes / No
  • What samples you are bringing and are most interested in?

Checkout Instructions
If only registering for a class, select any method of shipment to proceed, as shipping charges will not apply.

If ordering products in addition to registration, select desired shipping method.

Regular Tuition Registration, June Course: $2,950.00 Materials Ultramicrotomy Workshop
Regular Tuition Registration, July Course: $2,950.00 Materials Ultramicrotomy Workshop
Regular Tuition Registration, August Course: $2,950.00 Materials Ultramicrotomy Workshop

Pay by Check

Make payable to EMS and specify "June Course", "July Course" or "August Course".

Return your registration to:
Stacie Kirsch
1560 Industry Road
Hatfield, PA 19440 USA

Please contact Stacie Kirsch for more information.
Phone: 215-412-8400 Fax: 215-412-8450
E-Mail: stacie@ems-secure.com