Prepared Staining Kits for Histology and Pathology
F. CONNECTIVE TISSUE STAINS
F.1 Van Gieson Stains and related

Van Gieson's Method for Collagen Fibers
Mallory (1938); Van Gieson (1889)
For collagen, muscle and cornified epithelium.
Stain results:
Collagen: |
|
Red |
Smooth and striated muscle: |
|
Yellowish to Brownish |
Cornified epithelium: |
|
Yellow |
Hyalin: |
|
Yellow |
Nuclei: |
|
Blue to Black |
References:
- Clark, G., (ed) Staining Procedures, 3rd Ed., Williams & Wilkins Co., Baltimore, c. 1973, p. 50
- Luna, L. (ed) AFIP Manual o f Histological Staining Methods, 3rd ED., New York: McGraw Hill Publications, c 1968, p. 76
- Van Gieson, I., New York Med. J., 50:57-60, 1889.
- Mallory, F.B., Pathological Technique, Hafner Publishing., New York, c. 1961, p. 152
RT |
26350-01 |
Van Gieson's Solution |
 |
500 ml |
36.00 |
Add to Cart |
RT |
26350-02 |
Weigert's Iron Hematoxylin A |
 |
500 ml |
46.00 |
Add to Cart |
RT |
26350-03 |
Weigert's Iron Hematoxylin B |
 |
500 ml |
28.00 |
Add to Cart |

Puchtler-Sweat Method for Basement Membranes
Puchtler and Sweat (1964)
Stain results:
Basement Membranes: |
|
Black in cross section/
Gray in tangential sections |
Nuclei: |
|
Pink to Red |
References:
- Puchtler, H. and Sweat, F.: Stain Technology, 39:163, 1964
- AFIP Manual of Histological Staining Mehtods, 3rd ed., Ed. L. Luna: NewYork, McGraw Hill Pub., c. 1968, p. 73
RT |
26356-01 |
Resorcin-Fuchsin Solution |
 |
500 ml |
46.00 |
Add to Cart |
RT |
26356-02 |
Nuclear Fast Red (Kernechtrot) Solution |
 |
500 ml |
50.00 |
Add to Cart |
RT |
26356-03 |
Periodic Acid, 0.5% Aqueous |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26356-04 |
Sodium Bisulfite Solution |
|
500 ml |
32.00 |
Add to Cart |

Picrosirius Red Staining of Cardiac
Muscle Following Phosphomolybdic Acid Treatment
For connective tissue stains – Van Gieson stains and variants.
Stain results:
References:
- Dolber, Paul C. and Madison S. Spach: Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment, Stain Technology, Vol. 62 No. 1 (1987) p. 23-26
RT |
26357-01 |
Phosphomolybdic Acid, 0.2% Aqueous |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26357-02 |
Sirius Red, 0.1% in Saturated Picric Acid |
 |
100 ml |
12.00 |
Add to Cart |
RT |
26357-03 |
Hydrochloric Acid, 0.01N |
 |
500 ml |
17.00 |
Add to Cart |
F.2 Stains with Poly-Acid Step

Lillie Modification of Masson's Trichrome
Lillie (1940)
Stains cells, cytoplasm muscle and collagen of mammalian tissue
Stain results:
Nuclei: |
|
Black |
Cystoplasm: |
|
Brown to Pink |
Muscle: |
|
Red |
Erythrocytes: |
|
Brilliant Scarlet |
Collagen: |
|
Blue to Blue Green |
Myelinated fibers: |
|
Red |
Fibrin: |
|
Red to Pink |
References:
- Clark, G.: Staining Procedures. Williams & Wilkins Co., Baltimore, 3rd ed., c. 1973, p. 59
- Lillie, R.D., Arch. Path., 29:705, 1940
RT |
26364-01 |
Phosphomolybdic/ Phosphotungstic Acid Solution |
 |
500 ml |
66.00 |
Add to Cart |
RT |
26364-02 |
Weigert's Iron Hematoxylin A |
 |
500 ml |
45.00 |
Add to Cart |
RT |
26364-03 |
Weigert's Iron Hematoxylin B |
 |
500 ml |
27.00 |
Add to Cart |
RT |
26364-04 |
Biebrich Scarlet Solution, 1% |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26364-05 |
Fast Green FCF Solution, 2.5% |
 |
500 ml |
49.00 |
Add to Cart |
RT |
26364-06 |
Acetic Acid, 1% Aqueous |
 |
500 ml |
19.00 |
Add to Cart |
RT |
26364-07 |
Lugol's Iodine |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26364-7A |
Gram's Iodine Solution |
 |
500 ml |
20.00 |
Add to Cart |
RT |
26364-08 |
Sodium Thiosulfate, 5% Aqueous |
 |
500 ml |
22.00 |
Add to Cart |

Masson's Trichrome for Connective Tissue
Sheehan and Hrapchak (1980)
For stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc.
Stain results:
Nuclei: |
|
Black |
Cytoplasm, Keratin, Muscle Fibers: |
|
Red |
Collagen, Mucin: |
|
Blue |
References:
- Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190
RT |
26367-01 |
Bouin's Fixative |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26367-02 |
Weigert's Iron Hematoxylin A |
 |
500 ml |
46.00 |
Add to Cart |
RT |
26367-03 |
Weigert's Iron Hematoxylin B |
 |
500 ml |
33.00 |
Add to Cart |
RT |
26367-04 |
Biebrich Scarlet Solution, 1% |
 |
500 ml |
44.00 |
Add to Cart |
RT |
26033-25 |
Biebrich Scarlet – Acid Fuchsin |
 |
500 ml |
48.00 |
Add to Cart |
RT |
26367-05 |
Phosphomolybdic/ Phosphotungstic Acid Solution |
 |
500 ml |
66.00 |
Add to Cart |
RT |
26367-06 |
Aniline Blue Solution |
 |
500 ml |
60.00 |
Add to Cart |
RT |
26367-07 |
Acetic Acid, 1% Aqueous |
 |
500 ml |
18.00 |
Add to Cart |
F.3 Elastic Tissue Stains

Modified Verhoff Elastic Van Gieson Stain
For connective tissue stains – Stain with a poly-acid step.
Stain results:
Nuclei: |
|
Grayish-Black |
Collagen Fibers: |
|
Red |
Elastic Fibers: |
|
Bluish-Black |
Muscle: |
|
Greenish-Yellow |
References:
- Gravey, Winsome et al "A modified Verhoff Elastic Van Gieson Stain" The Journal of Histotechnology, Vol. 14, No. 2, pp 113-114. June 1991
RT |
26369-01 |
Alcoholic Hematoxylin, 3% |
 |
500 ml |
63.00 |
Add to Cart |
RT |
26369-02 |
Ferric Chloride, 2% aqueous |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26369-03 |
Lugol's Iodine |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26369-04 |
Ferric Chloride, 0.4% aqueous |
 |
500 ml |
17.00 |
Add to Cart |
RT |
26369-05 |
Van Geison's Solution |
 |
250 ml |
21.00 |
Add to Cart |

Weigert's Resorcin Fuchsin
Lillie (1965); Mallory (1938); Weigert (1898)
For stain elastic fibers in blood vessel walls, nuclei and collagen.
Stain results:
Elastic Tissues: |
|
Dark Blue |
Nuclei (red if Orth's Carmine used): |
|
Dark Blue |
Collagen: |
|
Red-Pink |
Miscellaneous Tissue Elements: |
|
Yellow (with Van Gieson's stain) |
References:
- Mallory, F.B.: Pathological Technique; New York: Hafner Publishing Co., c. 1961, p. 168
- Clark G.: Staining Procedures, Baltimore: Williams & Wilkins Co., 3rd ed. c. 1973, p. 60
- Luna, I.G.: AFIP Histologic Staining Methods: New York: McGraw Hill Book Co., 3rd ed., c. 1968, p. 80
RT |
26370-01 |
Resorcin-Fuchsin Solution |
 |
500 ml |
53.00 |
Add to Cart |
RT |
26370-02 |
Van Gieson's Solution |
 |
500 ml |
36.00 |
Add to Cart |
RT |
26370-03 |
Weigert's Iron Hematoxylin A |
 |
500 ml |
47.00 |
Add to Cart |
RT |
26370-04 |
Weigert's Iron Hematoxylin B |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26370-05 |
Acid Alcohol, 1% |
 |
500 ml |
22.00 |
Add to Cart |

Verhoeff's Van-Gieson's (VVG Method)
Sheehan and Hrapchak (1980)
For stain elastic fibers, nuclei, collagen and other tissue elements.
Stain results:
Elastic Fibers: |
|
Blue-black to Black – Fine elastic fibrils
may not be stained with this method |
Nuclei: |
|
Blue to Black |
Collagen: |
|
Red |
Other Tissue Elements: |
|
Yellow |
References:
- Sheehan, Dezna C., Hrapchack, Barbara B.: Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby C., St Louis, p. 196-197, c. 1980
RT |
26374-01 |
Alcoholic Hematoxylin, 5% |
 |
500 ml |
70.00 |
Add to Cart |
RT |
26374-02 |
Ferric Chloride, 10% |
 |
500 ml |
30.00 |
Add to Cart |
RT |
26374-03 |
Weigert's Iodine |
 |
500 ml |
42.00 |
Add to Cart |
RT |
26374-04 |
Ferric Chloride, 2% |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26374-05 |
Sodium Thiosulfate, 5% Aqueous |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26374-06 |
Van-Gieson's Solution |
 |
500 ml |
36.00 |
Add to Cart |

Carstairs Method for Fibrin and Platelets
Carstairs (1965)
For the staining of fibrin and platelets.
Stain results:
|
48 hours or more |
Less than 48 hours |
Fibrin: |
Bright Red |
Orange to Orange-Red |
Platelets |
Gray blue to navy |
Light Gray |
Collagen: |
Bright Blue |
Bright Blue |
Muscle: |
Red |
Red |
Red Blood Cells: |
Clear Yellow |
Red, Green or Yellow |
RT |
26381-01 |
Ferric Ammonium Sulfate, 5% Aqueous |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26381-02 |
Mayer's Hematoxylin |
 |
500 ml |
46.00 |
Add to Cart |
RT |
26381-03 |
Picric Acid-Orange G Solution |
 |
500 ml |
43.00 |
Add to Cart |
RT |
26381-04 |
Ponceau Acid Fuchsin Solution |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26381-05 |
Phosphotungstic Acid, 1% |
 |
500 ml |
44.00 |
Add to Cart |
RT |
26381-06 |
Aniline Blue Solution |
 |
500 ml |
50.00 |
Add to Cart |

Modified Movat's Stain
For connective tissue stains – Elastic tissue stains.
Stain results:
Nuclei: |
|
Black |
Cytoplasm: |
|
Red |
Elastic Fibers: |
|
Purple to Black |
Collagen and Bone: |
|
Yellow |
Mucopolysaccharides: |
|
Blue-Green |
Muscle: |
|
Red |
References:
- Movat, H.Z.: Demonstration of all connective tissue elements in a single section. Arch Path., 60:289-295, 1955
- Roman N., et al: Orcein-hematoxylin in iodized ferric chloride as a stain for elastic tissue. Stain Technology, 42:199-202, 1967
RT |
26385-01 |
Alcian Blue, 1% |
 |
100 ml |
28.00 |
Add to Cart |
RT |
26385-02 |
Alkaline Alcohol |
 |
500 ml |
33.00 |
Add to Cart |
RT |
26385-03 |
Orcein, 0.2% |
 |
250 ml |
28.00 |
Add to Cart |
RT |
26385-04 |
Hematoxylin Alcoholic, 5% |
 |
250 ml |
43.00 |
Add to Cart |
RT |
26385-05 |
Ferric Chloride, 10% |
 |
500 ml |
30.00 |
Add to Cart |
RT |
26385-06 |
Lugol's Iodine |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26385-07 |
Woodstain Scarlet-Acid Fuchsin Working Solution |
 |
250 ml |
17.00 |
Add to Cart |
RT |
26385-08 |
Acetic Acid, 0.5% |
 |
500 ml |
18.00 |
Add to Cart |
RT |
26385-09 |
Phosphotungstic Acid, 5% |
 |
500 ml |
42.00 |
Add to Cart |
RT |
26385-10 |
Alcoholic Saffron, 6% |
 |
100 ml |
44.00 |
Add to Cart |

Goldner's Trichrome Method
For connective tissue stains – Elastic tissue stain. Stain with a poly-acid step.
Stain results:
Nuclear chromatin: |
|
Brownish-Black |
Cytoplasm: |
|
Bright Red |
Erythrocytes: |
|
Orange |
Muscle: |
|
Red |
Collagen: |
|
Green |
References:
- Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Johnson Printers, Downerts Grove, IL pp 151-152, c 1992
RT |
26386-01 |
Bouin's Fluid |
 |
500 ml |
28.00 |
Add to Cart |
RT |
26386-02 |
Weigert's Iron Hematoxylin A |
 |
500 ml |
44.00 |
Add to Cart |
RT |
26386-03 |
Weigert's Iron Hematoxylin B |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26386-04 |
Ponceau Acid Fuchsin |
 |
100 ml |
11.00 |
Add to Cart |
RT |
26386-05 |
Acetic Acid, 1% Aqueous |
 |
500 ml |
16.00 |
Add to Cart |
RT |
26386-06 |
Phosphomolybdic Acid Orange G Solution |
 |
250 ml |
26.00 |
Add to Cart |
RT |
26386-07 |
Light Green Stock, 0.2% |
 |
500 ml |
32.00 |
Add to Cart |
F.4 Silver Methods

Wilder Modification of Bielschowsky's Method
Wilder (1935)
For the staining of Collagen and reticulum.
Stain results:
Reticulum Fibers (If beading is observed and/or a dark
background, replace the ammonium hydroxide silver
Nitrate solution and the reducing solution): |
|
Black |
Collagen: |
|
Rose |
Other Tissue Elements: |
|
Red |
References:
- Wilder, H.C., Amer. J. Clin. Path.: 11:817, 1935
- Mallory, F.B.: Pathological Techniques, Phil., W.B. Saunders & Co. c. 1938
- AFIP Manual of Hitological Staining Techniques, NY, McGraw-Hill Publ., c. 1968/3rd ed, p. 92
RT |
26392-01 |
Silver Nitrate, 10.2% Aqueous |
 |
250 ml |
101.00 |
Add to Cart |
RT |
26392-02 |
Ammonium Hydroxide, Concentrate |
 |
500 ml |
18.00 |
Add to Cart |
RT |
26392-03 |
Sodium Hydroxide, 3.1% Aqueous |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26392-04 |
Formalin, 40%, Neutralized |
 |
500 ml |
27.00 |
Add to Cart |
RT |
26392-05 |
Harris Hematoxylin
OR |
|
500 ml |
32.00 |
Add to Cart |
RT |
26392-5A |
Van-Gieson Stain |
|
500 ml |
36.00 |
Add to Cart |
RT |
26392-06 |
Nuclear Fast Red (Kernechtrot) Solution |
 |
500 ml |
49.00 |
Add to Cart |
RT |
26392-07 |
Phosphomolybdic Acid, 10% Aqueous |
 |
500 ml |
81.00 |
Add to Cart |
RT |
26392-08 |
Uranium Nitrate, 1% Aqueous |
 |
500 ml |
137.00 |
Add to Cart |
RT |
26392-09 |
Gold Chloride, 0.2%, Aqueous |
 |
250 ml |
52.00 |
Add to Cart |
RT |
26392-10 |
Sodium Thiosulfate, 5% Aqueous |
|
500 ml |
20.00 |
Add to Cart |

Jones Method for Kidney
- Silver Method
For the staining of basement membranes, reticulum fibers, collagen and nuclei.
Stain results:
Basement membranes, reticulum fibers: |
|
Black |
Nuclei: |
|
Blue |
Cytoplasm, collagen, connective tissue: |
|
Pink-Orange |
References:
- Jones, D.B., Amer. J. Path. 27:99 (1951)
- AFIP Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97
RT |
26396-01 |
Periodic Acid, 0.5%, Aqueous |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26396-02 |
Methenamine Solution, 3%, Aqueous |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26396-03 |
Silver Nitrate, 5% Aqueous Solution |
 |
250 ml |
84.00 |
Add to Cart |
RT |
26396-04 |
Borate Buffer Working Solution |
 |
500 ml |
29.00 |
Add to Cart |
RT |
26396-05 |
Gold Chloride, 0.2%, Aqueous |
 |
250 ml |
55.00 |
Add to Cart |
RT |
26396-06 |
Harris's Hematoxylin |
 |
500 ml |
34.00 |
Add to Cart |
RT |
26396-07 |
Alcoholic Eosin Y Staining Solution |
 |
500 ml |
29.00 |
Add to Cart |
RT |
26396-09 |
Sodium Thiosulfate, 3%, Aqueous |
 |
500 ml |
20.00 |
Add to Cart |
RT |
26396-11 |
Potassium Ferricyanide, 0.5%, Aqueous |
 |
500 ml |
20.00 |
Add to Cart |
RT |
26396-12 |
Sodium Metabisulfite, 3%, Aqueous |
 |
500 ml |
28.00 |
Add to Cart |
RT |
26396-13 |
Acid Alcohol, 1% |
 |
500 ml |
21.00 |
Add to Cart |
RT |
26396-14 |
Ammonia Water, 0.3% |
 |
500 ml |
15.00 |
Add to Cart |
F.5 Keratin Stains

Dane's Method for Prekeratin, Keratin, and Mucin
Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei.
Stain results:
Acid Mucopolysaccharides: |
|
Blue |
Prekeratin and Keranin: |
|
Orange to Red |
Nuclei: |
|
Brown |
References:
- Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963
- AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p. 83
RT |
26401-01 |
Phloxine Solution, 1%, Aqueous |
 |
500 ml |
27.00 |
Add to Cart |
RT |
26401-02 |
Alcian Blue Solution, 0.5% |
 |
250 ml |
53.00 |
Add to Cart |
RT |
26401-03 |
Orange G Solution |
 |
500 ml |
42.00 |
Add to Cart |
RT |
26401-04 |
Mayer's Hematoxylin |
 |
500 ml |
58.00 |
Add to Cart |