Prepared Staining Kits for Histology and Pathology

F. CONNECTIVE TISSUE STAINS

F.1 Van Gieson Stains and related

Van Gieson's Method for Collagen Fibers

Mallory (1938); Van Gieson (1889)

For collagen, muscle and cornified epithelium.

Stain results:

Collagen:		Red
Smooth and striated muscle:		Yellowish to Brownish
Cornified epithelium:		Yellow
Hyalin:		Yellow
Nuclei:		Blue to Black

References:

• Clark, G., (ed) Staining Procedures, 3rd Ed., Williams & Wilkins Co., Baltimore, c. 1973, p. 50
• Luna, L. (ed) AFIP Manual o f Histological Staining Methods, 3rd ED., New York: McGraw Hill Publications, c 1968, p. 76
• Van Gieson, I., New York Med. J., 50:57-60, 1889.
• Mallory, F.B., Pathological Technique, Hafner Publishing., New York, c. 1961, p. 152

RT	26350-01	Van Gieson's Solution		500 ml	34.00	Add to Cart
RT	26350-02	Weigert's Iron Hematoxylin A		500 ml	46.00	Add to Cart
RT	26350-03	Weigert's Iron Hematoxylin B		500 ml	26.00	Add to Cart

Puchtler-Sweat Method for Basement Membranes

Puchtler and Sweat (1964)

Stain results:

Basement Membranes:		Black in cross section/ Gray in tangential sections
Nuclei:		Pink to Red

References:

• Puchtler, H. and Sweat, F.: Stain Technology, 39:163, 1964
• AFIP Manual of Histological Staining Mehtods, 3rd ed., Ed. L. Luna: NewYork, McGraw Hill Pub., c. 1968, p. 73

RT	26356-01	Resorcin-Fuchsin Solution		500 ml	46.00	Add to Cart
RT	26356-02	Nuclear Fast Red (Kernechtrot) Solution		500 ml	46.00	Add to Cart
RT	26356-03	Periodic Acid, 0.5% Aqueous		500 ml	24.00	Add to Cart
RT	26356-04	Sodium Bisulfite Solution		500 ml	32.00	Add to Cart

Picrosirius Red Staining of Cardiac

Muscle Following Phosphomolybdic Acid Treatment

For connective tissue stains – Van Gieson stains and variants.

Stain results:

Septa:

Red

References:

• Dolber, Paul C. and Madison S. Spach: Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment, Stain Technology, Vol. 62 No. 1 (1987) p. 23-26

RT	26357-01	Phosphomolybdic Acid, 0.2% Aqueous		500 ml	23.00	Add to Cart
RT	26357-02	Sirius Red, 0.1% in Saturated Picric Acid		100 ml	11.00	Add to Cart
RT	26357-03	Hydrochloric Acid, 0.01N		500 ml	17.00	Add to Cart

F.2 Stains with Poly-Acid Step

Lillie Modification of Masson's Trichrome

Lillie (1940)

Stains cells, cytoplasm muscle and collagen of mammalian tissue

Stain results:

Nuclei:		Black
Cystoplasm:		Brown to Pink
Muscle:		Red
Erythrocytes:		Brilliant Scarlet
Collagen:		Blue to Blue Green
Myelinated fibers:		Red
Fibrin:		Red to Pink

References:

• Clark, G.: Staining Procedures. Williams & Wilkins Co., Baltimore, 3rd ed., c. 1973, p. 59
• Lillie, R.D., Arch. Path., 29:705, 1940

RT	26364-01	Phosphomolybdic/ Phosphotungstic Acid Solution		500 ml	66.00	Add to Cart
RT	26364-02	Weigert's Iron Hematoxylin A		500 ml	45.00	Add to Cart
RT	26364-03	Weigert's Iron Hematoxylin B		500 ml	27.00	Add to Cart
RT	26364-04	Biebrich Scarlet Solution, 1%		500 ml	24.00	Add to Cart
RT	26364-05	Fast Green FCF Solution, 2.5%		500 ml	46.00	Add to Cart
RT	26364-06	Acetic Acid, 1% Aqueous		500 ml	18.00	Add to Cart
RT	26364-07	Lugol's Iodine		500 ml	23.00	Add to Cart
RT	26364-7A	Gram's Iodine Solution		500 ml	19.00	Add to Cart
RT	26364-08	Sodium Thiosulfate, 5% Aqueous		500 ml	20.00	Add to Cart

Masson's Trichrome for Connective Tissue

Sheehan and Hrapchak (1980)

For stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc.

Stain results:

Nuclei:		Black
Cytoplasm, Keratin, Muscle Fibers:		Red
Collagen, Mucin:		Blue

References:

• Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190

RT	26367-01	Bouin's Fixative		500 ml	24.00	Add to Cart
RT	26367-02	Weigert's Iron Hematoxylin A		500 ml	42.00	Add to Cart
RT	26367-03	Weigert's Iron Hematoxylin B		500 ml	32.00	Add to Cart
RT	26367-04	Biebrich Scarlet Solution, 1%		500 ml	43.00	Add to Cart
RT	26367-05	Phosphomolybdic/ Phosphotungstic Acid Solution		500 ml	65.00	Add to Cart
RT	26367-06	Aniline Blue Solution		500 ml	60.00	Add to Cart
RT	26367-07	Acetic Acid, 1% Aqueous		500 ml	17.00	Add to Cart

F.3 Elastic Tissue Stains

Modified Verhoff Elastic Van Gieson Stain

For connective tissue stains – Stain with a poly-acid step.

Stain results:

Nuclei:		Grayish-Black
Collagen Fibers:		Red
Elastic Fibers:		Bluish-Black
Muscle:		Greenish-Yellow

References:

• Gravey, Winsome et al "A modified Verhoff Elastic Van Gieson Stain" The Journal of Histotechnology, Vol. 14, No. 2, pp 113-114. June 1991

RT	26369-01	Alcoholic Hematoxylin, 3%		500 ml	63.00	Add to Cart
RT	26369-02	Ferric Chloride, 2% aqueous		500 ml	20.00	Add to Cart
RT	26369-03	Lugol's Iodine		500 ml	25.00	Add to Cart
RT	26369-04	Ferric Chloride, 0.4% aqueous		500 ml	16.00	Add to Cart
RT	26369-05	Van Geison's Solution		250 ml	21.00	Add to Cart

Weigert's Resorcin Fuchsin

Lillie (1965); Mallory (1938); Weigert (1898)

For stain elastic fibers in blood vessel walls, nuclei and collagen.

Stain results:

Elastic Tissues:		Dark Blue
Nuclei (red if Orth's Carmine used):		Dark Blue
Collagen:		Red-Pink
Miscellaneous Tissue Elements:		Yellow (with Van Gieson's stain)

References:

• Mallory, F.B.: Pathological Technique; New York: Hafner Publishing Co., c. 1961, p. 168
• Clark G.: Staining Procedures, Baltimore: Williams & Wilkins Co., 3rd ed. c. 1973, p. 60
• Luna, I.G.: AFIP Histologic Staining Methods: New York: McGraw Hill Book Co., 3rd ed., c. 1968, p. 80

RT	26370-01	Resorcin-Fuchsin Solution		500 ml	50.00	Add to Cart
RT	26370-02	Van Gieson's Solution		500 ml	34.00	Add to Cart
RT	26370-03	Weigert's Iron Hematoxylin A		500 ml	43.00	Add to Cart
RT	26370-04	Weigert's Iron Hematoxylin B		500 ml	28.00	Add to Cart
RT	26370-05	Acid Alcohol, 1%		500 ml	22.00	Add to Cart

Verhoeff's Van-Gieson's (VVG Method)

Sheehan and Hrapchak (1980)

For stain elastic fibers, nuclei, collagen and other tissue elements.

Stain results:

Elastic Fibers:		Blue-black to Black – Fine elastic fibrils may not be stained with this method
Nuclei:		Blue to Black
Collagen:		Red
Other Tissue Elements:		Yellow

References:

• Sheehan, Dezna C., Hrapchack, Barbara B.: Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby C., St Louis, p. 196-197, c. 1980

RT	26374-01	Alcoholic Hematoxylin, 5%		500 ml	66.00	Add to Cart
RT	26374-02	Ferric Chloride, 10%		500 ml	27.00	Add to Cart
RT	26374-03	Weigert's Iodine		500 ml	40.00	Add to Cart
RT	26374-04	Ferric Chloride, 2%		500 ml	23.00	Add to Cart
RT	26374-05	Sodium Thiosulfate, 5% Aqueous		500 ml	22.00	Add to Cart
RT	26374-06	Van-Gieson's Solution		500 ml	33.00	Add to Cart

Carstairs Method for Fibrin and Platelets

Carstairs (1965)

For the staining of fibrin and platelets.

Stain results:

	48 hours or more	Less than 48 hours
Fibrin:	Bright Red	Orange to Orange-Red
Platelets	Gray blue to navy	Light Gray
Collagen:	Bright Blue	Bright Blue
Muscle:	Red	Red
Red Blood Cells:	Clear Yellow	Red, Green or Yellow

RT	26381-01	Ferric Ammonium Sulfate, 5% Aqueous		500 ml	26.00	Add to Cart
RT	26381-02	Mayer's Hematoxylin		500 ml	44.00	Add to Cart
RT	26381-03	Picric Acid-Orange G Solution		500 ml	41.00	Add to Cart
RT	26381-04	Ponceau Acid Fuchsin Solution		500 ml	25.00	Add to Cart
RT	26381-05	Phosphotungstic Acid, 1%		500 ml	43.00	Add to Cart
RT	26381-06	Aniline Blue Solution		500 ml	48.00	Add to Cart

Modified Movat's Stain

For connective tissue stains – Elastic tissue stains.

Stain results:

Nuclei:		Black
Cytoplasm:		Red
Elastic Fibers:		Purple to Black
Collagen and Bone:		Yellow
Mucopolysaccharides:		Blue-Green
Muscle:		Red

References:

• Movat, H.Z.: Demonstration of all connective tissue elements in a single section. Arch Path., 60:289-295, 1955
• Roman N., et al: Orcein-hematoxylin in iodized ferric chloride as a stain for elastic tissue. Stain Technology, 42:199-202, 1967

RT	26385-01	Alcian Blue, 1%		100 ml	26.00	Add to Cart
RT	26385-02	Alkaline Alcohol		500 ml	30.00	Add to Cart
RT	26385-03	Orcein, 0.2%		250 ml	26.00	Add to Cart
RT	26385-04	Hematoxylin Alcoholic, 5%		250 ml	40.00	Add to Cart
RT	26385-05	Ferric Chloride, 10%		500 ml	27.00	Add to Cart
RT	26385-06	Lugol's Iodine		500 ml	25.00	Add to Cart
RT	26385-07	Woodstain Scarlet-Acid Fuchsin Working Solution		250 ml	16.00	Add to Cart
RT	26385-08	Acetic Acid, 0.5%		500 ml	17.00	Add to Cart
RT	26385-09	Phosphotungstic Acid, 5%		500 ml	40.00	Add to Cart
RT	26385-10	Alcoholic Saffron, 6%		100 ml	40.00	Add to Cart

Goldner's Trichrome Method

For connective tissue stains – Elastic tissue stain. Stain with a poly-acid step.

Stain results:

Nuclear chromatin:		Brownish-Black
Cytoplasm:		Bright Red
Erythrocytes:		Orange
Muscle:		Red
Collagen:		Green

References:

• Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Johnson Printers, Downerts Grove, IL pp 151-152, c 1992

RT	26386-01	Bouin's Fluid		500 ml	27.00	Add to Cart
RT	26386-02	Weigert's Iron Hematoxylin A		500 ml	40.00	Add to Cart
RT	26386-03	Weigert's Iron Hematoxylin B		500 ml	30.00	Add to Cart
RT	26386-04	Ponceau Acid Fuchsin		100 ml	11.00	Add to Cart
RT	26386-05	Acetic Acid, 1% Aqueous		500 ml	16.00	Add to Cart
RT	26386-06	Phosphomolybdic Acid Orange G Solution		250 ml	25.00	Add to Cart
RT	26386-07	Light Green Stock, 0.2%		500 ml	32.00	Add to Cart

F.4 Silver Methods

Wilder Modification of Bielschowsky's Method

Wilder (1935)

For the staining of Collagen and reticulum.

Stain results:

Reticulum Fibers (If beading is observed and/or a dark
background, replace the ammonium hydroxide silver

Nitrate solution and the reducing solution):		Black
Collagen:		Rose
Other Tissue Elements:		Red

References:

• Wilder, H.C., Amer. J. Clin. Path.: 11:817, 1935
• Mallory, F.B.: Pathological Techniques, Phil., W.B. Saunders & Co. c. 1938
• AFIP Manual of Hitological Staining Techniques, NY, McGraw-Hill Publ., c. 1968/3rd ed, p. 92

RT	26392-01	Silver Nitrate, 10.2% Aqueous		250 ml	101.00	Add to Cart
RT	26392-02	Ammonium Hydroxide, Concentrate		500 ml	18.00	Add to Cart
RT	26392-03	Sodium Hydroxide, 3.1% Aqueous		500 ml	23.00	Add to Cart
RT	26392-04	Formalin, 40%, Neutralized		500 ml	27.00	Add to Cart
RT	26392-05	Harris Hematoxylin OR		500 ml	32.00	Add to Cart
RT	26392-5A	Van-Gieson Stain		500 ml	33.00	Add to Cart
RT	26392-06	Nuclear Fast Red (Kernechtrot) Solution		500 ml	46.00	Add to Cart
RT	26392-07	Phosphomolybdic Acid, 10% Aqueous		500 ml	81.00	Add to Cart
RT	26392-08	Uranium Nitrate, 1% Aqueous		500 ml	137.00	Add to Cart
RT	26392-09	Gold Chloride, 0.2%, Aqueous		250 ml	52.00	Add to Cart
RT	26392-10	Sodium Thiosulfate, 5% Aqueous		500 ml	20.00	Add to Cart

Jones Method for Kidney

- Silver Method

For the staining of basement membranes, reticulum fibers, collagen and nuclei.

Stain results:

Basement membranes, reticulum fibers:		Black
Nuclei:		Blue
Cytoplasm, collagen, connective tissue:		Pink-Orange

References:

• Jones, D.B., Amer. J. Path. 27:99 (1951)
• AFIP Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97

RT	26396-01	Periodic Acid, 0.5%, Aqueous		500 ml	32.00	Add to Cart
RT	26396-02	Methenamine Solution, 3%, Aqueous		500 ml	26.00	Add to Cart
RT	26396-03	Silver Nitrate, 5% Aqueous Solution		250 ml	84.00	Add to Cart
RT	26396-04	Borate Buffer Working Solution		500 ml	27.00	Add to Cart
RT	26396-05	Gold Chloride, 0.2%, Aqueous		250 ml	55.00	Add to Cart
RT	26396-06	Harris's Hematoxylin		500 ml	34.00	Add to Cart
RT	26396-07	Alcoholic Eosin Y Staining Solution		500 ml	26.00	Add to Cart
RT	26396-09	Sodium Thiosulfate, 3%, Aqueous		500 ml	19.00	Add to Cart
RT	26396-11	Potassium Ferricyanide, 0.5%, Aqueous		500 ml	19.00	Add to Cart
RT	26396-12	Sodium Metabisulfite, 3%, Aqueous		500 ml	27.00	Add to Cart
RT	26396-13	Acid Alcohol, 1%		500 ml	20.00	Add to Cart
RT	26396-14	Ammonia Water, 0.3%		500 ml	14.00	Add to Cart

F.5 Keratin Stains

Dane's Method for Prekeratin, Keratin, and Mucin

Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei.

Stain results:

Acid Mucopolysaccharides:		Blue
Prekeratin and Keranin:		Orange to Red
Nuclei:		Brown

References:

• Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963
• AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p. 83

RT	26401-01	Phloxine Solution, 1%, Aqueous		500 ml	26.00	Add to Cart
RT	26401-02	Alcian Blue Solution, 0.5%		250 ml	52.00	Add to Cart
RT	26401-03	Orange G Solution		500 ml	40.00	Add to Cart
RT	26401-04	Mayer's Hematoxylin		500 ml	54.00	Add to Cart

Cytoplasmic Granule Stains