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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

arrow13Microscope Graticles and Stage Micrometers

EMS is proud to add to our already extensive line -- a new wide range of Microscopy Calibration Standards and Standard Graticules, which are manufactured in the USA . This new line adheres to extremely tight tolerances and all of them are available with or without Secondary or Direct NIST certification.

arrow12A.   Linear Reference Scales

We offer Chrome-on-Glass (G) and Chrome-on-Opal glass (O) or Chrome-on Quartz (Q).  The Opal and Quartz are offered as a special only and must be requested.

P: positive image (mostly clear or white); N: negative image (mostly opaque or black)

All EMS micrometers come in a sturdy wooden box.

arrow12Model SM-1
60210-1PG.jpg
Glass Size: 2” x 5.25” (50 mm x 132 mm)
Scale Length:   5 inch (English) X-axis only
125 mm (Metric) X-axis only
Divisions:  English = 0.01”; Metric = 100µm (0.1mm)
With numerical labeling every 1 mm and 0.1”
60210-1PG Stage Micrometer, SM-1, chrome on glass each 525.00 Add to Cart
60210-1PGC Stage Micrometer, SM-1 with Calibration each 1,280.00 Add to Cart
arrow12Model SM-3

60210-3PG.jpg

Glass Size: 1” x 3” (25mm x 75mm). ≥1.42 mm (0.056”) thickness
Scale Length:   0.04 inch (English) X & Y axis
10 mm (Metric) X &Y axis
Divisions:  English = 0.0005”; Metric = 10µm (0.010mm)
With numerical labeling every 0.1 mm and 0.05”
Flatness: ≤ 10 µm / 100 mm
Image Forming Material: Chrome
Image Contrast/Density: Chrome transmission density is ≥ 2.0
Feature Size Accuracy: 2 µm
Image Placement Accuracy: 0.001 mm / 100 mm
Polarity: Scales are opaque on a clear background
Reading Direction: Right Read Chrome Up (RRCU)
Typical Uses: SM-3 is useful for calibration of magnification in microscopes and other optical magnification systems. Its auto-location features can be used for testing of an image analysis auto-location system.
60210-3PG Stage Micrometer, SM-3, chrome on glass each 340.00 Add to Cart
60210-3PGC Stage Micrometer, SM-3 with Calibration each 1,295.00 Add to Cart
arrow12Model SM-4

60210-4PG.jpg

Glass Size: 1” x 3” (25mm x 75mm)
Scale Length:   1 inch (English) X-axis only
25 mm (Metric)   X-axis only
Divisions:  English = 0.0005”; Metric = 10µm (0.010 mm)
With numerical labeling every 0.1 mm and 0.05”
60210-4PG Stage Micrometer, SM-4, Chrome on glass each 320.00 Add to Cart
60210-4PGC Stage Micrometer, SM-4 with Calibration each 1,200.00 Add to Cart
arrow12Model SM-5

60210-5PG.jpg

Glass Size: 1.5” x 3” (38mm x 75mm)
Scale Length:   1 inch (English) X & Y axis
25 mm (Metric) X & Y axis
Divisions:  English = 0.001”; Metric = 25µm (0.025 mm)
With numerical labeling every 1 mm and 0.1”
60210-5PG Stage Micrometer, SM-5, Chrome on glass each 479.00 Add to Cart
60210-5PGC Stage Micrometer, SM-5 with Calibration each 1,100.00 Add to Cart
arrow12Model SM-6
Same scale as SM-5, but smaller division.
Glass Size: 1.5” x 3” (38mm x 75mm).
Scale Length:   1 inch (English) X & Y axis
25 mm (Metric) X & Y axis
Divisions:  English = 0.0005”; Metric = 10µm (0.010 mm)
With numerical labeling every 0.1 mm and 0.05”
60210-6PG Stage Micrometer, SM-6, Chrome on Glass each 560.00 Add to Cart
60210-6PGC Stage Micrometer, SM-6 with Calibration each 1,200.00 Add to Cart
60210-12PG.jpgarrow12Model SM-12
Glass Size: 1” x 3” (25mm x 75mm).
≥1.42 mm (0.056”) thickness
Scale Length:   0.08 inch (English) X-axis only
2.0 mm (Metric) X-axis only
Divisions:  English = 0.001”; Metric = 25µm (0.025 mm)
No numerical scale labeling
Flatness: ≤ 10 µm / 100 mm
Image Forming Material: Chrome
Image Contrast/Density: Chrome transmission density is ≥ 2.0
Feature Size Accuracy: 2 µm
Image Placement Accuracy 0.001 mm / 100 mm
Polarity Scales are opaque on a clear background
Reading Direction Right Read Chrome Up (RRCU)
Typical Uses SM-12 is useful for calibration of magnification in microscopes and other optical magnification systems.
60210-12PG Stage Micrometer, SM-12, chrome on glass each 230.00 Add to Cart
60210-12PGC Stage Micrometer, SM-12 with Calibration each 750.00 Add to Cart
arrow12Model SM-13

60210-13PG.jpg

Glass Size: 1” x 3” (25mm x 75mm)
Scale Length:   0.08 inch (English) X & Y axis
2.0 mm (Metric) X & Y axis
Divisions:  English = 0.001”; Metric = 25µm (0.025 mm)
No numerical scale labeling
60210-13PG Stage Micrometer, SM-13, Chrome on glass each 295.00 Add to Cart
60210-13PGC Stage Micrometer, SM-13 with Calibration each 900.00 Add to Cart

arrow12B.  Image Analysis & Microscopy (IAM)

Vision Calibration Standards

We are proud to introduce the first complete and true line of Image Analysis Microscopy Calibration Standards, specially designed to address the current needs of the new generation of image analysis and machine vision systems. These unique standards are designed to test multiple morphometric parameters to assure that your image analysis system is working properly; meeting the required calibration and certification requirements of your vision measurement systems.

We offer Chrome-on-Glass (G) and Chrome-on-Opal glass (O) or Chrome on Quartz (Q). The Opal and Quartz are offered by request only as a custom.

P: positive image (mostly clear or white); N: negative image (mostly opaque or black)

arrow12Model IAM-1 – Morphometric Image Analysis Standards

60216-1PG.jpg

Ideally for morphological calibration where two dimensional shapes have to be measured precisely.  Standard includes a set of eight test plates of various shapes, positions, angles and sizes. It also includes a linear measurement scale for calibrating your imaging system. Overall size: 1” x 3” (25mm x 75mm). Positive image / Chrome on glass (PG)

Plate Frame Size Description
1 4600 x 3500µm  Circles – 2000, 1000, 500 & 250 µm diameters
2 1000 x 800 µm Circles – 500, 250, 125 & 62.5 µm diameters
3 1000 x 800 µm Bars – 200x20 µm at 30 degrees increments
4 1000 x 800 µm Various Shapes, Circles, Bars & Angular designs.
5 1000 x 800 µm Squares – 100, 40, 20 µm set of 2 each.
6 2050 x 1650 µm Grid Patterns – 50 µm lines at 100 µm pitch
7 4200 x 3400 µm Grid Patterns – 200 µm lines at 200 µm pitch
8 10 mm long Scale with 10 µm divisions
60216-1PG Morphology Stage Micrometer, Model IAM-1 each 425.00 Add to Cart
60216-1PGC Morphology Stage Micrometer, Model IAM-1, T/L, Calibrated only plates 1, 2 & 8 each 1,075.00 Add to Cart
arrow12Model IAM-MET

ASTM Grain Size / Nodularity Analysis Standard

60216-METC.jpg

Based on the IAM-1 Morphometric Image Analysis above, the IAM-MET is a multi-morphology calibration standard designed specifically for ASTM. The IAM-MET includes all plates as found in IAM-1 except for plate 4, which is pictured above. This plate contains 9 images of irregular shapes, simulating features commonly found in metallurgical applications. As required by the ASTM standard, the IAM-MET includes secondary certification of  plates 1, 2 and 8.

Overall size: 76.2 mm x 25.4 mm (3” x 1”)
Image: Chrome on glass / positive. Calibrated 32 points.
60216-METC Model IAM-MET, ASTM Grain Size, 32 pts Calibrated each 699.00 Add to Cart
arrow12Model IAM-2 – Linear (X & Y axis) Scale

60216-2PG.jpg

A dual axis linear micrometer that can accurately calibrate both X & Y directions without rotation, for optical magnification, and camera aspect ratio using single standard. In addition, it can be used to calibrate and check linear stage motion or related auto measuring systems. Scales are in both English and Metric measurements.

Overall size: 1” x 3” (25 mm x 75 mm)
Scale Image: 1” (right scale); 25 mm (left scale)
Y = 0.60” (right scale); 15 mm (left scale)
Divisions: English = 0.001”, Metric = 25µm  
Image: Positive image / Chrome on glass (PG)
60216-2PG Linear (X&Y axis) Stage Micrometer, Model IAM-2 each 325.00 Add to Cart
60216-2PGC Linear (X&Y axis) Stage Micrometer, Model IAM-2, T/L, Calibrated each 1,100.00 Add to Cart
arrow12Model IAM-5 – Stage Mapping – Grid Pattern

60216-5SNG.jpg

This ideal standard is for verifying or qualifying multiple image analysis parameters such as optical distortion, alignment between systems or optical paths, and cell areas for particle counting. The image area is 20mm x 50mm.

Overall size: 1” x 3” (25mm x 75mm)
Image Forming Material:  Negative image / Chrome on glass (NG)
Model No. Clear Aperture   Opaque Lines Pitch
IAM-5S 8.5 µm square 1.5 µm square 10 µm
IAM-5M 180 µm square 20 µm square 200 µm
IAM-5L 980 µm square 20 µm square 1000 µm
60216-5SNG Model IAM-5S, Grids, Small Size, 10µm Pitch each 525.00 Add to Cart
60216-5SNGC Model IAM-5S with Calibrated (12 pts) each 1,040.00 Add to Cart
60216-5MNG Model IAM-5M, Grids, Medium Size, 200µm Pitch each 425.00 Add to Cart
60216-5MNGC Model IAM-5M with Calibrated (12 pts) each 950.00 Add to Cart
60216-5LNG Model IAM-5L, Grids, Large Size, 1000µm Pitch each 295.00 Add to Cart
60216-5LNGC Model IAM-5L with Calibrated (12 pts) each 875.00 Add to Cart
arrow12Model IAM-8 – Multi Grid Standard

60216-8PG.jpg

A uniqulye designed standard with 4 different grid size patterns: 0.5 x 0.5 mm grid with 25 µm pitch; 1.0 x 1.0 mm grid with 50 µm pitch; 2.0 x 2.0 grid with 100 µm pith; and 4.0 x 4.0 mm grid with 200 µm pitch. Also includes a Star Target with 15 degree angle increments. This standard can be used to test not only the overall frame distortion issue, but also linear distances for a wide variety of magnification ranges from 1x to 1000x power.

Overall Size 1” x 3” (25mm x 75mm)
Image:   Positive image / Chrome on glass (PG)
60216-8PG Model IAM-8, Multi Grids Standard each 250.00 Add to Cart
60216-8PGC Model IAM-8 Calibrated (16 pts) each 600.00 Add to Cart
arrow12ACM-1 – Calibration Micrometer

60218-1PG.jpg

This ideal standard provides accurate calibration of any eyepiece, reticules, filar micrometers, or electronic measuring instrument. Features a series of ‘H’ shaped fiducial images in a range of sizes from 0.1 mm through 20 mm, useful for calibration range from 1X to 1000X power.

Standard Size:  25 mm x 75 mm
Substrate Type: Transparent glass (G), 1.42 mm or thicker
Opal glass (O) is available per request.
Image Forming Material: Evaporated chromium on glass
Image Description: ‘H’ shaped fiducials as follows, centerline to centerline
Pattern Length (mm) Pattern Height Line Width
0.10, 0.25, 0.50, 0.75 0.50 mm 10 µm
1.00, 2.50, 5.00, 7.50 1.00 mm 20 µm
10.0, 15.0, 20.0 3.00 mm 40 µm
Polarity: Positive (opaque or black features on clear background)
Reading Direction: Right Read Chrome Up (RRCU)
Image Placement Accuracy: 3.0 microns or better
Image Contrast/Density: Chrome optical density grater than 2.0
Typical Use: Verification or calibration of magnification.
60218-1PG Model ACM-1, Calibrating Micrometer each 290.00 Add to Cart
60218-1PGC Model ACM-1, with 11 pts Calibrated each 600.00 Add to Cart

arrow12C.  Color Transmission Calibration

arrow12NIST Traceable Color Transmission Calibration Slide - Calibrated

Color Transmission Calibration Slide

Microscopy color patches for assessing color accuracy. NIST traceable calibration data for each large color patch is supplied as spectral transmission. By using the supplied spreadsheet, this data can be converted to the color space you need (such as Adobe RGB 1998, L*A*B*, XYZ, etc.)

-24 color patches plus large clear area

-Black background to minimize flare

-The 4150 µm diameter patches are individually calibrated for spectral transmission. This allows accurate conversion (using the supplied spreadsheet) to other color space units.

-Smaller patches from 1000 µm diameter to 150 µm diameter are exposed and processed at the same time produce good correlation of the large calibrated patches to the smaller ones, but are not calibrated.

-Oil immersion objectives can be used because a standard 0.15 mm glass cover slip is permanently cemented over the color film.

-Film is permanently sandwiched between glass; atmospheric deterioration of the measured values is minimized.

-Each slide is individually serialized and calibrated.

Specifications

Overall Size 75mm x 25mm
Feature Array 24 unique color patches in 9 different sizes, ranging from 4.150 mm diameter to 0.150 mm diameter, and a large clear film area. Only the large patches (and clear area) are directly calibrated, but all patches are imaged and processed at the same time.
Polarity Negative
Material 0.2 mm (0.007") thick polyester photo-film mounted onto 0.9 to 1 mm water white Soda-lime Glass with a 0.15 mm (0.006") glass coverslip for protection (exact thickness is subject to change)

Includes

  • Calibrated IAM-9C color microscope slide
  • Calibration Certificate
  • Calibration Data Report
  • Conversion Spreadsheet
  • USB flash drive with data and spreadsheet
  • Protective case
79504 NIST Traceable Color Transmission Calibration Slide - Calibrated each 1,250.00 Add to Cart

Standard Reticules arrow13arrow13