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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

Downloadable PDFarrow13Microscope Graticles and Stage Micrometers

EMS is proud to add to our already extensive line -- a new wide range of Microscopy Calibration Standards and Standard Graticules, which are manufactured in the USA. This new line adheres to extremely tight tolerances and all of them are available with or without Secondary or Direct NIST certification.

arrow12A. Linear Reference Scales

Wooden Case

We offer Chrome-on-Glass (G) and Chrome-on-Opal glass (O) or Chrome-on Quartz (Q). The Opal and Quartz are offered as a special only and must be requested.

P: positive image (mostly clear or white); N: negative image (mostly opaque or black)

All EMS micrometers come in a sturdy wooden case.

arrow12Model SM-1

Stage Micrometer, SM-1

5 inch/125 mm X-Axis Linear Stage Micrometer

The SM-1 linear stage micrometer is a versatile microscopy measurement scale. At 5" in length, with 100µm divisions and NIST traceable calibration, the SM-1 offers a large measurement scale length with precise divisions in small increments. Labeling at 1 mm and .1" intervals.

Specifications

Glass Size 2" x 5.25" (50 mm x 132 mm)
Scale Length 5 inch (English) X-axis only
125 mm (Metric) X-axis only
Divisions English = 0.01"; Metric = 100µm (0.1 mm)
With numerical labeling every 1 mm and 0.1"

Ordering

60210-1PG Stage Micrometer, SM-1, Chrome on Glass each 525.00 Add to Cart
60210-1PGC Stage Micrometer, SM-1, with Calibration each 1,280.00 Add to Cart
arrow12Model SM-2
Stage Micrometer, SM-2

Labeled 0.08 inch/2 mm X-Axis Linear Stage Micrometer

The SM-2 is a 2 mm X-axis scale with .0127 mm divisions, making ideal for higher magnification microscopes, or smaller fields of view. Labeling is every 0.01".

Specifications

Glass Size 1" x 3" (25 mm x 75 mm)
Scale Length 0.08 inch (English) X-axis only
2.0 mm (Metric) X-axis only
Divisions English = 0.0005"; Metric = 10µm (0.01 mm)

Ordering

60210-2PG Stage Micrometer, SM-2, Chrome on Glass each 229.00 Add to Cart
60210-2PGC Stage Micrometer, SM-2, with Calibration each 640.00 Add to Cart
arrow12Model SM-3

Stage Micrometer, SM-3

0.40 inch/10 mm X&Y Axis Linear Stage Micrometer

The SM-3 is useful for calibration of magnification in microscopes and other optical magnification systems. Its autolocation features can be used for testing of an image analysis auto-location system.

Specifications

Glass Size 1" x 3" (25 mm x 75 mm). ≥1.42 mm (0.056") thickness
Scale Length 0.04 inch (English) X & Y axis
10 mm (Metric) X &Y axis
Divisions English = 0.0005"; Metric = 10µm (0.01 mm)
With numerical labeling every 0.1 mm and 0.05"
Image Forming Material Chrome
Image Contrast/Density Chrome transmission density is ≥ 2.0
Flatness ≤ 10 µm/100 mm
Feature Size Accuracy 2 µm
Image Placement Accuracy 0.001 mm/100 mm
Polarity Scales are opaque on a clear background
Reading Direction Right Read Chrome Up (RRCU)

Ordering

60210-3PG Stage Micrometer, SM-3, Chrome on Glass each 340.00 Add to Cart
60210-3PGC Stage Micrometer, SM-3, with Calibration each 1,295.00 Add to Cart
arrow12Model SM-4

Stage Micrometer, SM-4

1.0 inch/25 mm X-Axis Linear Stage Micrometer

The SM-4 is a one inch linear X axis scale and our most commonly used stage micrometer. Combined with NIST traceable calibration, the SM-4 allows you to be efficient and precise in your measurements. The superior line edge quality at 10 µm divisions makes it the preferred choice for high power magnification.

Specifications

Glass Size 1" x 3" (25 mm x 75 mm)
Scale Length 1 inch (English) X-axis only
25 mm (Metric) X-axis only
Divisions English = 0.0005"; Metric = 10µm (0.01 mm)
With numerical labeling every 0.1 mm and 0.05"

Ordering

60210-4PG Stage Micrometer, SM-4, Chrome on Glass each 320.00 Add to Cart
60210-4PGC Stage Micrometer, SM-4, with Calibration each 1,200.00 Add to Cart
arrow12Model SM-5

Stage Micrometer, SM-5

1.0 inch/25 mm X&Y Axis Linear Scale (0.001"/ 25µm div.)

The SM-5 is an ideal slide for measuring X & Y axis subjects at multiple power levels in either English or Metric scales. A truly versatile slide, it is precision imaged for the sharpest line edge quality and offered with NIST traceability to improve the accuracy and ease of your measurements.

Specifications

Glass Size 1.5" x 3" (38 mm x 75 mm)
Scale Length 1 inch (English) X & Y axis
25 mm (Metric) X & Y axis
Divisions English = 0.001"; Metric = 25µm (0.025 mm)
With numerical labeling every 1 mm and 0.1"

Ordering

60210-5PG Stage Micrometer, SM-5, Chrome on Glass each 479.00 Add to Cart
60210-5PGC Stage Micrometer, SM-5, with Calibration each 1,100.00 Add to Cart
arrow12Model SM-6

Stage Micrometer, SM-6

1.0 inch/25 mm X&Y Axis Linear Scale (0.0005"/10µm div.)

Same scale as SM-5, but smaller division.

Specifications

Glass Size 1.5" x 3" (38 mm x 75 mm).
Scale Length 1 inch (English) X & Y axis
25 mm (Metric) X & Y axis
Divisions English = 0.0005"; Metric = 10µm (0.01 mm)
With numerical labeling every 0.1 mm and 0.05"

Ordering

60210-6PG Stage Micrometer, SM-6, Chrome on Glass each 560.00 Add to Cart
60210-6PGC Stage Micrometer, SM-6, with Calibration each 1,300.00 Add to Cart
arrow12Model SM-12

Stage Micrometer, SM-12

0.08 inch/2.0 mm X-Axis Linear Scale

The SM-12 is useful for calibration of magnification in microscopes and other optical magnification systems.

Specifications

Glass Size 1" x 3" (25 mm x 75 mm). ≥1.42 mm (0.056") thickness
Scale Length 0.08 inch (English) X-axis only
2.0 mm (Metric) X-axis only
Divisions English = 0.001"; Metric = 25µm (0.025 mm)
No numerical scale labeling
Flatness ≤ 10 µm/100 mm
Image Forming Material Chrome
Image Contrast/Density Chrome transmission density is ≥ 2.0
Feature Size Accuracy 2 µm
Image Placement Accuracy 0.001 mm/100 mm
Polarity Scales are opaque on a clear background
Reading Direction Right Read Chrome Up (RRCU)

Ordering

60210-12PG Stage Micrometer, SM-12, Chrome on Glass each 230.00 Add to Cart
60210-12PGC Stage Micrometer, SM-12, with Calibration each 750.00 Add to Cart
arrow12Model SM-13

Stage Micrometer, SM-13

0.08 inch/2.0 mm X&Y Axis Linear Scale

No numerical scale labeling. The SM-12 is useful for calibration of magnification in microscopes and other optical magnification systems.

Specifications

Glass Size 1" x 3" (25 mm x 75 mm)
Scale Length 0.08 inch (English) X & Y axis
2.0 mm (Metric) X & Y axis
Divisions English = 0.001"; Metric = 25µm (0.025 mm)
No numerical scale labeling

Ordering

60210-13PG Stage Micrometer, SM-13, Chrome on Glass each 295.00 Add to Cart
60210-13PGC Stage Micrometer, SM-13, with Calibration each 900.00 Add to Cart

arrow12B. Image Analysis & Microscopy (IAM)

Vision Calibration Standards

We are proud to introduce the first complete and true line of Image Analysis Microscopy Calibration Standards, specially designed to address the current needs of the new generation of image analysis and machine vision systems. These unique standards are designed to test multiple morphometric parameters to assure that your image analysis system is working properly; meeting the required calibration and certification requirements of your vision measurement systems.

We offer Chrome-on-Glass (G) and Chrome-on-Opal glass (O) or Chrome on Quartz (Q). The Opal and Quartz are offered by request only as a custom.

P: positive image (mostly clear or white); N: negative image (mostly opaque or black).

arrow12Model IAM-1

Morphology Stage Micrometer, Model IAM-1

Morphometric Image Analysis Standards

Ideally for morphological calibration where two dimensional shapes have to be measured precisely. Standard includes a set of eight test plates of various shapes, positions, angles and sizes. It also includes a linear measurement scale for calibrating your imaging system. Overall size: 1" x 3" (25 mm x 75 mm). Positive image/Chrome on glass (PG).

Specifications

Plate Frame Size Description
1 4,600 x 3,500µm Circles – 2,000, 1,000, 500 & 250 µm diameters
2 1,000 x 800 µm Circles – 500, 250, 125 & 62.5 µm diameters
3 1,000 x 800 µm Bars – 200 x 20 µm at 30° increments
4 1,000 x 800 µm Various Shapes, Circles, Bars & Angular designs
5 1,000 x 800 µm Squares – 100, 40, 20 µm set of 2 each.
6 2,050 x 1,650 µm Grid Patterns – 50 µm lines at 100 µm pitch
7 4,200 x 3,400 µm Grid Patterns – 200 µm lines at 200 µm pitch
8 10 mm long Scale with 10 µm divisions

Ordering

60216-1PG Morphology Stage Micrometer, Model IAM-1 each 425.00 Add to Cart
60216-1PGC Morphology Stage Micrometer, Model IAM-1, T/L, Calibrated only plates 1, 2 & 8 each 1,075.00 Add to Cart
arrow12Model IAM-MET

Model IAM-MET, ASTM

ASTM Grain Size/Nodularity Analysis Standard

Based on the IAM-1 Morphometric Image Analysis above, the IAM-MET is a multi-morphology calibration standard designed specifically for ASTM. The IAM-MET includes all plates as found in IAM-1 except for plate 4, which is pictured above. This plate contains 9 images of irregular shapes, simulating features commonly found in metallurgical applications. As required by the ASTM standard, the IAM-MET includes secondary certification of plates 1, 2 and 8.

Specifications

Overall size 76.2 mm x 25.4 mm (3" x 1")
Image Chrome on glass/positive. Calibrated 32 points.

Ordering

60216-METC Model IAM-MET, ASTM Grain Size, 32 pts, Calibrated each 699.00 Add to Cart
arrow12Model IAM-2

Linear (X&Y axis) Stage Micrometer, Model IAM-2

Linear (X & Y axis) Scale

A dual axis linear micrometer that can accurately calibrate both X & Y directions without rotation, for optical magnification, and camera aspect ratio using single standard. In addition, it can be used to calibrate and check linear stage motion or related auto measuring systems. Scales are in both English and Metric measurements.

Specifications

Overall Size 1" x 3" (25 mm x 75 mm)
Scale Image 1" (right scale); 25 mm (left scale)
Y = 0.60" (right scale); 15 mm (left scale)
Divisions English = 0.001", Metric = 25µm (0.025 mm)
Image Positive image/Chrome on glass (PG)

Ordering

60216-2PG Linear (X&Y axis) Stage Micrometer, Model IAM-2 each 325.00 Add to Cart
60216-2PGC Linear (X&Y axis) Stage Micrometer, Model IAM-2, T/L, Calibrated each 1,100.00 Add to Cart
arrow12Model IAM-3

Ultra-High Image Analysis Resolution Target, Model IAM-3

Ultra-High Image Analysis Resolution Target

Based on the NIST/NBS 1010A and conforming to ANSI/ISO#2 standard, this target is ideal for verifying focus and resolution of high powered optical systems. With direct read information, the exact capabilities of the system will instantly be established. The values (in cycles per mm) are displayed next to 5 vertical and 5 horizontal evenly spaced bars, from 5.6 to 512 cycles per mm (89.2 µm to 0.98 µm bar sizes).

Specifications

Overall Size 1" x 3" (25 mm x 75 mm)
Resolution 5.5 to 512 cy/mm

Ordering

60216-3PG Ultra-High Image Analysis Resolution Target, Model IAM-3 each 540.00 Add to Cart
60216-3PGC Ultra-High Image Analysis Resolution Target, Model IAM-3, Calibrated each 880.00 Add to Cart
arrow12Model IAM-4

Image Analysis Neutral Density Step Tablet, Model IAM-4

Image Analysis Neutral Density Step Tablet

Designed for illumination and detection calibration, which is needed for today's exacting imaging and vision systems. This tablet has 12 Transmission Steps, nominally ranging from 0 to 100% transmission. The neutral density step tablet is ideal for testing linearity of the sensing system.

Specifications

Overall Size 1" x 3" (25 mm x 75 mm)
Material Emulsion film on glass
Density Range 0.08 to 2.00

Ordering

60216-4PG Image Analysis Neutral Density Step Tablet, Model IAM-4 each 360.00 Add to Cart
60216-4PGC Image Analysis Neutral Density Step Tablet, Model IAM-4, Calibrated each 700.00 Add to Cart
arrow12Model IAM-5

Model IAM-5

Stage Mapping – Grid Pattern

This ideal standard is for verifying or qualifying multiple image analysis parameters such as optical distortion, alignment between systems or optical paths, and cell areas for particle counting. The image area is 20 mm x 50 mm.

Model No. Clear Aperture Opaque Lines Pitch
IAM-5S 8.5 µm square 1.5 µm square 10 µm
IAM-5M 180 µm square 20 µm square 200 µm
IAM-5L 980 µm square 20 µm square 1,000 µm

Specifications

Overall Size 1" x 3" (25 mm x 75 mm)
Image Forming Material Negative image/Chrome on glass (NG)

Ordering

60216-5SNG Model IAM-5S, Grids, Small Size, 10µm Pitch each 525.00 Add to Cart
60216-5SNGC Model IAM-5S, with Calibrated (12 pts) each 1,040.00 Add to Cart
60216-5MNG Model IAM-5M, Grids, Medium Size, 200µm Pitch each 425.00 Add to Cart
60216-5MNGC Model IAM-5M, with Calibrated (12 pts) each 950.00 Add to Cart
60216-5LNG Model IAM-5L, Grids, Large Size, 1,000µm Pitch each 295.00 Add to Cart
60216-5LNGC Model IAM-5L, with Calibrated (12 pts) each 875.00 Add to Cart
arrow12Model IAM-6

Image Analysis Micro Line/Dot Standard, Model IAM-6

Image Analysis Micro Line/Dot Standard

A standard specifically designed to calibrate CCD, CMOS devices and other geometric measuring imaging systems where critical measurement is important. Electronic sensors often have spurious resolution created by pixel dithering. The IAM-6 limits this calibration problem by providing known dot and line sizes to test the sensor/optical capabilities.

Specifications

Overall Size 1" x 3" (25 mm x 75 mm)
Material Positive image/Chrome on glass (PG)

Ordering

60216-6PG Image Analysis Micro Line/Dot Standard, Model IAM-6 each 360.00 Add to Cart
60216-6PGC Image Analysis Micro Line/Dot Standard, Model IAM-6, Calibrated each 605.00 Add to Cart
arrow12Model IAM-8

Ultra-High Image Analysis Resolution Target, Model IAM-8

Multi-Grid Image Analysis Standard

A uniquely designed standard with 4 different grid size patterns: 0.5 x 0.5 mm grid with 25 µm pitch; 1.0 x 1.0 mm grid with 50 µm pitch; 2.0 x 2.0 grid with 100 µm pith; and 4.0 x 4.0 mm grid with 200 µm pitch. Also includes a Star Target with 15 degree angle increments. This standard can be used to test not only the overall frame distortion issue, but also linear distances for a wide variety of magnification ranges from 1x to 1,000x power.

Specifications

Overall Size 1" x 3" (25 mm x 75 mm)
Image Positive image/Chrome on glass (PG)

Ordering

60216-8PG Ultra-High Image Analysis Resolution Target, Model IAM-8 each 250.00 Add to Cart
60216-8PGC Ultra-High Image Analysis Resolution Target, Model IAM-8, Calibrated each 600.00 Add to Cart
arrow12ACM-1

Model ACM-1

Calibration Micrometer

This ideal standard provides accurate calibration of any eyepiece, reticules, filar micrometers, or electronic measuring instrument. Features a series of 'H' shaped fiducial images in a range of sizes from 0.1 mm through 20 mm, useful for calibration range from 1x to 1,000x power.

Specifications

Standard Size 25 mm x 75 mm
Substrate Type Transparent glass (G), 1.42 mm or thicker
Opal glass (O) is available per request
Image Forming Material Evaporated chromium on glass
Image Description 'H' shaped fiducials as follows, centerline to centerline
Pattern Length (mm) Pattern Height Line Width
0.10, 0.25, 0.50, 0.75 0.50 mm 10 µm
1.00, 2.50, 5.00, 7.50 1.00 mm 20 µm
10.0, 15.0, 20.0 3.00 mm 40 µm
Polarity Positive
Reading Direction Right Read Chrome Up (RRCU)
Image Placement Accuracy 3.0 microns or better
Image Contrast/Density Chrome optical density grater than 2.0

Ordering

60218-1PG Calibration Micrometer, Model ACM-1 each 310.00 Add to Cart
60218-1PGC Calibration Micrometer with 11 pts Calibrated, Model ACM-1 each 600.00 Add to Cart

arrow12C. Color Transmission Calibration

NIST Traceable Color Transmission Calibration Slide

NIST Traceable Color Transmission Calibration Slide – Calibrated

Microscopy color patches for assessing color accuracy. NIST traceable calibration data for each large color patch is supplied as spectral transmission. By using the supplied spreadsheet, this data can be converted to the color space you need (such as Adobe RGB 1998, L*A*B*, XYZ, etc.).

  • 24 color patches plus large clear area
  • Black background to minimize flare
  • The 4150 µm diameter patches are individually calibrated for spectral transmission. This allows accurate conversion (using the supplied spreadsheet) to other color space units.
  • Smaller patches from 1,000 µm diameter to 150 µm diameter are exposed and processed at the same time produce good correlation of the large calibrated patches to the smaller ones, but are not calibrated.
  • Oil immersion objectives can be used because a standard 0.15 mm glass cover slip is permanently cemented over the color film.
  • Film is permanently sandwiched between glass; atmospheric deterioration of the measured values is minimized.
  • Each slide is individually serialized and calibrated.

 

Specifications

Overall Size 75 mm x 25 mm
Feature Array 24 unique color patches in 9 different sizes, ranging from 4.15 mm diameter to 0.15 mm diameter, and a large clear film area. Only the large patches (and clear area) are directly calibrated, but all patches are imaged and processed at the same time.
Polarity Negative
Material 0.2 mm (0.007") thick polyester photo-film mounted onto 0.9 to 1 mm water white Soda-lime Glass with a 0.15 mm (0.006") glass coverslip for protection (exact thickness is subject to change)

Includes

  • Calibrated IAM-9C color microscope slide
  • Calibration Certificate
  • Calibration Data Report
  • Conversion Spreadsheet
  • USB flash drive with data and spreadsheet
  • Protective case

Ordering

79504 NIST Traceable Color Transmission Calibration Slide - Calibrated each 1,250.00 Add to Cart

Standard Reticules arrow13arrow13