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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

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arrow13NIGHTSEA™ for Hirox

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NIGHTSEA™ / EMS 2018 KEY Award

arrow12NIGHTSEA™ Fluorescence Adapter for Hirox Digital MicroscopeNightsea for Hirox Image GalleryNightsea for Hirox

The NIGHTSEA Model SFA Fluorescence Adapter system can add a versatile fluorescence imaging capability to the Hirox digital microscope. Visit our gallery of images made with the NIGHTSEA system and the Hirox microscope.

Fluorescence solutions

The key elements of any fluorescence system are:

  • a light source that produces sufficient energy in the appropriate wavelength range to excite fluorescence in the sample of interest
  • a barrier filter in the viewing path that blocks reflected excitation light while transmitting the fluorescence emitted by the sample

NIGHTSEA implements these for the Hirox system with:

  • high intensity LED light sources available in five excitation wavelength ranges (see list further down page)
  • emission barrier filters that can be added to the Hirox lenses easily and non-invasively
  • Hirox microscope with NIGHTSEA fluorescence adapter
  • Detail of NIGHTSEA light head and barrier filter with Hirox microscope
Nightsea for Hirox Image Gallery

Here are samples of what you can see. For many more examples visit our Hirox image gallery.

  • Epoxy on motor shaft, 20x, UV excitation
  • NConcrete thin section, 20x, white light and fluorescence under Royal Blue excitation
  • Conformal coating on circuit board, 20x, fluorescence under UV excitation and white light light
  • Bone in sand, 20x, fluorescence under UV excitation and white light
  • Fluorescent mineral, 20x, white light and fluorescence under Royal Blue excitation

Comprehensive solution kit

We have configured a versatile Hirox fluorescence adapter kit that includes the most useful excitation wavelengths for varied industry applications. The SFA-H-IND kit includes the following items, at a package price that is significantly less than purchasing the items separately:

  • Three excitation light heads – Ultraviolet (UV), Violet (VI), and Royal Blue (RB)
    • This recommendation is based on our experience with varied applications
  • Three barrier filters paired to the excitation light heads
  • Matching barrier filter glasses for each excitation wavelength
  • Gooseneck lamp base with dimming control
  • Universal power supply – 120/240VAC, 50/60Hz, with international plug set
  • Packing/carrying case with custom-cut foam

We offer additional wavelength sets that may be of use for other applications. See table below*

Why we recommend a set of three excitation wavelengths for industry needs

We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However, we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:

  • A prospective customer wanted to image concrete thin sections impregnated with fluorescent dye. The dye supplier recommended UV, and it is true that UV will make the dye fluoresce nicely. We found that our Royal Blue excitation produced significantly brighter fluorescence.
  • Similar to above, except that a fluorescence epoxy had been used to highlight surface cracks in a test sample. Royal Blue excitation yielded brighter fluorescence.
  • A prospective customer sent us a sample of material that was known to fluoresce under UV. The goal was to see the distribution of non-fluorescent particles in a fluorescent matrix. While both UV and Royal Blue excited fluorescence in the sample, the best imaging contrast was achieved with Violet excitation.

Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise. Contact us if you would like to discuss your application further.

*Wavelength sets

There are five excitation/emission wavelength combinations available for the system.

Color Designation Excitation Emission
Ultraviolet UV 360 - 380nm 415nm longpass
Violet VI 400 - 415nm 450nm longpass
Royal Blue RB 440 - 460nm 500nm longpass
Cyan CY 490 - 515nm 550nm longpass
Green GR 510 - 540nm 600nm longpass

Ordering Information

arrow12Best Value Full System Kit

Includes three wavelengths (UV + VI + RB)

SFA-H-IND Best Value Full System for Hirox kit 2,850.00 Add to Cart
arrow12Single wavelength full systems

Available in non-UV or UV wavelengths

SFA-H-VI Hirox Single Wavelength Full System - Violet kit 1,345.00 Add to Cart
SFA-H-RB Hirox Single Wavelength Full System - Royal Blue kit 1,345.00 Add to Cart
SFA-H-CY Hirox Single Wavelength Full System - Cyan kit 1,345.00 Add to Cart
SFA-H-GR Hirox Single Wavelength Full System - Green kit 1,345.00 Add to Cart
SFA-H-UV Hirox Single Wavelength Full System - UV kit 1,495.00 Add to Cart
arrow12Add-on single wavelengths

Available in non-UV or UV wavelengths

SFA-H-LFS-VI Hirox Single Wavelength Add-On - Violet each 685.00 Add to Cart
SFA-H-LFS-RB Hirox Single Wavelength Add-On - Royal Blue each 685.00 Add to Cart
SFA-H-LFS-CY Hirox Single Wavelength Add-On - Cyan each 685.00 Add to Cart
SFA-H-LFS-GR Hirox Single Wavelength Add-On - Green each 685.00 Add to Cart
SFA-H-LFS-UV Hirox Single Wavelength Add-On UV each 835.00 Add to Cart
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