Nanopatterned Cell Cultureware
Parallel nanoscale topography aligns and matures cells and tissues
More mature cell and tissue cultures, faster
NanoSurface Cultureware structures cultured cells and tissues to improve physiological relevance. Shortly after plating, cells cultured in NanoSurface Cultureware exhibit enhanced structural and phenotypic development as compared to cells cultured in conventional culture dishes. NanoSurface topography induces cytoskeletal reorganization and cellular alignment. NanoSurface Cultureware is available in familiar standard formats, featuring No. 1.5 glass-bottom wells for high quality imaging.
Cells in the Dish Should Resemble Cells in the Body
NanoSurface plates rapidly structure and mature many cell types
- Cardiomyocytes
- Skeletal muscle cells
- Smooth muscle cells
- Endothelial cells
- Human embryonic stem cells
- Induced pluripotent stem cells
- Mesenchymal stem cells
- Fibroblasts
- Epithelial cells
- Cancer cells
- And many more...
Biocompatible
- Non-cytotoxic nanopatterened surface can be functionalized with a variety of ECM proteins
Surface Features
- Substrate rigidity 6.69 MPa
- Cell culture treated
- Gamma sterile
High Quality Imaging
- Compatible with high-magnification, high-NA transmitted light and fluorescence microscopy techniques
- Nanotopography maintains migratory phenotypes of cancer cells
NanoSurface Cultureware vs. Conventional Dish
NanoSurface Cultureware features a nanopatterned culture surface which provides a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, improving physiological relevance by promoting development. Cells can align, elongate, grow, and even migrate along the pattern while exhibiting more physiologically representative structural and functional phenotypes.
Conventional cultureware does not utilize biomimetic surface topography, which results in random structural orientation. The disorganized isotropic cell and tissue architectures result in immature functional phenotypes that do not reproduce in vivo function. These inaccuracies lead to imprecise, hard-to-reproduce results and wasted time and effort.
NanoSurface Dish | Conventional Dish | |
On NanoSurface dishes, cardiomyocytes align to the surface topography, elongate in the direction of the nanopattern, and exhibit uniform anisotropic contraction patterns, with enhanced contractile force and conduction velocity. | Without biomimetic surface topography, cardiomyocytes exhibit random orientation, disorganized contraction patterns, and immature functional phenotypes on conventional culture surface. |
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CDI iCell cardiomyocytes on a NanoSurface dish (left) vs. conventional dish (right). Cardiomyocytes elongate in the direction of the nanopattern, develop structurally organized cytoskeletal networks, anisotropic cell shapes, striated and physiologically-spaced sarcomeres, and exhibit polarized expression of gap junction proteins. These and other changes lead to cardiac cells with more physiological and mature electrical and mechanical properties such as faster action potential conduction in the direction of the nanopattern, and improved contraction force and velocity. | ||
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Endothelial cells on a NanoSurface dish (left) vs. conventional dish (right). Endothelial cells form aligned layers with physiological anisotropy, and exhibit lower expression of inflammatory cytokines. | ||
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Directed migration of cancer cells on a NanoSurface dish (left) vs. on a conventional dish (right). Glioblastoma cells grown on traditional flat cultureware lose their migratory phenotype in culture, while cells grown on patterned dishes maintain it, with migration directed along the length of the pattern. Images from Smith et. al. Cell Reports 15(12):2016. | ||
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Two-channel confocal image of CDI cortical neurons cultured on NanoSurface Cultureware (left) vs. on a conventional dish (right). On NanoSurface Cultureware, neurofilaments (red) align along the direction of the nanotopography while dendrites (MAP2 stain; green) do not. |
Specifications
Product Type |
Product Code |
Approximate Pattern Growth Area (cm2) |
Total Well Volume (µL) |
Working Volume (µL) |
25mm Coverglass | ANFS-CS25 | 4.90 | – | – |
35mm Single Dish | ANFS-0001 | 3.14 | 17000 | 3000 |
6-well Plate* | ANFS-0006 | 3.14 | 17000 | 3000 |
24-well Plate* | ANFS-0024 | 1.65 | 3400 | 1000 |
96-well Plate* | ANFS-0096 | 0.33 | 360 | 200 |
Biomimetic nanoscale surface topography imitates the aligned structure of the native extracellular matrix
NanoSurface nanopatterned culture surfaces provide a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, promoting cellular structural and functional development.
View our Cytostretchers.
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The underlying matrix of the native myocardium has an aligned architecture (scale bar 10µm) |
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A) NanoSurface Coverglass
NanoSurface coverglass features the same polymeric nanoscale topography found in NanoSurface culture dishes and plates.
Unpatterned control coverglass is also available: fabricated with same polymer and process as NanoSurface coverglass, without nanoscale topography.
- 0.16 to 0.19 mm thick
- 25mm diameter circular coverglass
- Polymeric surface
- Either NanoSurface parallel surface topography OR unpatterned control surface
64820-01 | 25 mm circular NanoSurface coverglass No 1.5 thickness 800:800:600 nm surface topography |
50/pk | 500.00 | Add to Cart |
64820-02 | 25 mm circular NanoSurface coverglass No 1.5 thickness Unpatterned "flat" control surface |
50/pk | 500.00 | Add to Cart |


B) 35 mm NanoSurface Dish
35mm glass-bottom dish with a polymeric nanopatterned surface topography.
- Glass-Bottom
- NanoSurface topography
- 0.16 to 0.19 mm thick
- 20mm Glass Diameter
- Gamma Sterilized
- Cell Culture Treated
64821-01 | 35 mm NanoSurface Dish 800:800:600 nm surface topography |
10/pk | 165.00 | Add to Cart |
64821-02 | 35 mm NanoSurface Dish 800:800:600 nm surface topography |
100/pk | 1,600.00 | Add to Cart |
64821-03 | 35 mm NanoSurface Dish Unpatterned "flat" control surface |
10/pk | 165.00 | Add to Cart |
64821-04 | 35 mm NanoSurface Dish Unpatterned "flat" control surface |
100/pk | 1,600.00 | Add to Cart |



C) NanoSurface Plates
6, 24 or 96-well glass-bottom plates with a polymeric nanopatterned surface topography.
- Glass-Bottom
- NanoSurface topography
- 0.16 to 0.19 mm thick
- 20mm Glass Diameter
- Gamma Sterilized
- Cell Culture Treated
64822-01 | 6-Well NanoSurface Plate | each | 112.00 | Add to Cart |
64822-02 | 6-Well NanoSurface Plate | 10/pk | 860.00 | Add to Cart |
64822-03 | 24-Well NanoSurface Plate | each | 165.00 | Add to Cart |
64822-04 | 24-Well NanoSurface Plate | 10/pk | 1,325.00 | Add to Cart |
64822-05 | 96-Well NanoSurface Plate | each | 235.00 | Add to Cart |
64822-06 | 96-Well NanoSurface Plate | 10/pk | 1,900.00 | Add to Cart |