Nanopatterned Cell Cultureware

Parallel nanoscale topography aligns and matures cells and tissues

More mature cell and tissue cultures, faster

NanoSurface Cultureware structures cultured cells and tissues to improve physiological relevance. Shortly after plating, cells cultured in NanoSurface Cultureware exhibit enhanced structural and phenotypic development as compared to cells cultured in conventional culture dishes. NanoSurface topography induces cytoskeletal reorganization and cellular alignment. NanoSurface Cultureware is available in familiar standard formats, featuring No. 1.5 glass-bottom wells for high quality imaging.

NanoSurface plates rapidly structure and mature many cell types

• Cardiomyocytes
• Skeletal muscle cells
• Smooth muscle cells
• Endothelial cells
• Human embryonic stem cells
• Induced pluripotent stem cells
• Mesenchymal stem cells
• Fibroblasts
• Epithelial cells
• Cancer cells
• And many more...

Biocompatible

• Non-cytotoxic nanopatterened surface can be functionalized with a variety of ECM proteins

Surface Features

• Substrate rigidity 6.69 MPa
• Cell culture treated
• Gamma sterile

High Quality Imaging

• Compatible with high-magnification, high-NA transmitted light and fluorescence microscopy techniques
• Nanotopography maintains migratory phenotypes of cancer cells

Conventional Dish		NanoSurface Dish
Without biomimetic surface topography, cardiomyocytes exhibit random orientation, disorganized contraction patterns, and immature functional phenotypes on conventional culture surface		On NanoSurface dishes, cardiomyocytes align to the surface topography, elongate in the direction of the nanopattern, and exhibit uniform anisotropic contraction patterns, with enhanced contractile force and conduction velocity
Human Endothelial Cells/Conventional		Human Endothelial Cells/NanoSurface
Vardiomyocytes/Conventional		Vardiomyocytes/NanoSurface

Biomimetic nanoscale surface topography imitates the aligned structure of the native extracellular matrix

NanoSurface nanopatterned culture surfaces provide a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, promoting cellular structural and functional development.

The underlying matrix of the native myocardium has an aligned architecture (scale bar 10µm)

A) NanoSurface Coverglass

NanoSurface coverglass features the same polymeric nanoscale topography found in NanoSurface culture dishes and plates.

Unpatterned control coverglass is also available: fabricated with same polymer and process as NanoSurface coverglass, without nanoscale topography.

• 0.16 to 0.19 mm thick
• 25mm diameter circular coverglass
• Polymeric surface
• Either NanoSurface parallel surface topography OR unpatterned control surface

B) 35 mm NanoSurface Dish

35mm glass-bottom dish with a polymeric nanopatterned surface topography.

• Glass-Bottom
• NanoSurface topography
• 0.16 to 0.19 mm thick
• 20mm Glass Diameter
• Gamma Sterilized
• Cell Culture Treated

C) NanoSurface Plates

6, 24 or 96-well glass-bottom plates with a polymeric nanopatterned surface topography.

• Glass-Bottom
• NanoSurface topography
• 0.16 to 0.19 mm thick
• 20mm Glass Diameter
• Gamma Sterilized
• Cell Culture Treated

Wells, Optical Bottom Plates, Cell Culture Inserts