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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow13Nanopatterned Cell Cultureware

Parallel nanoscale topography aligns and matures cells and tissues

PDF download availablePDF download availableMore mature cell and tissue cultures, faster

NanoSurface Cultureware structures cultured cells and tissues to improve physiological relevance. Shortly after plating, cells cultured in NanoSurface Cultureware exhibit enhanced structural and phenotypic development as compared to cells cultured in conventional culture dishes. NanoSurface topography induces cytoskeletal reorganization and cellular alignment. NanoSurface Cultureware is available in familiar standard formats, featuring No. 1.5 glass-bottom wells for high quality imaging.

Cells in the Dish Should Resemble Cells in the Body

NanoSurface plates rapidly structure and mature many cell types

  • CardiomyocytesNanoSurface Coverglass
  • Skeletal muscle cells
  • Smooth muscle cells
  • Endothelial cells
  • Human embryonic stem cells
  • Induced pluripotent stem cells
  • Mesenchymal stem cells
  • Fibroblasts
  • Epithelial cells
  • Cancer cells
  • And many more...

Biocompatible

  • Non-cytotoxic nanopatterened surface can be functionalized with a variety of ECM proteins

Surface Features

  • Substrate rigidity 6.69 MPa
  • Cell culture treated
  • Gamma sterile

High Quality Imaging

  • Compatible with high-magnification, high-NA transmitted light and fluorescence microscopy techniques
  • Nanotopography maintains migratory phenotypes of cancer cells

NanoSurface Cultureware vs. Conventional Dish

NanoSurface Cultureware features a nanopatterned culture surface which provides a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, improving physiological relevance by promoting development. Cells can align, elongate, grow, and even migrate along the pattern while exhibiting more physiologically representative structural and functional phenotypes.

Conventional cultureware does not utilize biomimetic surface topography, which results in random structural orientation. The disorganized isotropic cell and tissue architectures result in immature functional phenotypes that do not reproduce in vivo function. These inaccuracies lead to imprecise, hard-to-reproduce results and wasted time and effort.

NanoSurface Dish   Conventional Dish
On NanoSurface dishes, cardiomyocytes align to the surface topography, elongate in the direction of the nanopattern, and exhibit uniform anisotropic contraction patterns, with enhanced contractile force and conduction velocity.  

Without biomimetic surface topography, cardiomyocytes exhibit random orientation, disorganized contraction patterns, and immature functional phenotypes on conventional culture surface.

 
CDI iCell cardiomyocytes on a NanoSurface dish (left) vs. conventional dish (right). Cardiomyocytes elongate in the direction of the nanopattern, develop structurally organized cytoskeletal networks, anisotropic cell shapes, striated and physiologically-spaced sarcomeres, and exhibit polarized expression of gap junction proteins. These and other changes lead to cardiac cells with more physiological and mature electrical and mechanical properties such as faster action potential conduction in the direction of the nanopattern, and improved contraction force and velocity.
 
Endothelial cells on a NanoSurface dish (left) vs. conventional dish (right). Endothelial cells form aligned layers with physiological anisotropy, and exhibit lower expression of inflammatory cytokines.
 
Directed migration of cancer cells on a NanoSurface dish (left) vs. on a conventional dish (right). Glioblastoma cells grown on traditional flat cultureware lose their migratory phenotype in culture, while cells grown on patterned dishes maintain it, with migration directed along the length of the pattern. Images from Smith et. al. Cell Reports 15(12):2016.
 
Two-channel confocal image of CDI cortical neurons cultured on NanoSurface Cultureware (left) vs. on a conventional dish (right). On NanoSurface Cultureware, neurofilaments (red) align along the direction of the nanotopography while dendrites (MAP2 stain; green) do not.

Specifications



Product Type


Product Code
Approximate
Pattern Growth
Area (cm2)

Total Well
Volume (µL)

Working
Volume (µL)
25mm Coverglass ANFS-CS25 4.90
35mm Single Dish ANFS-0001 3.14 17000 3000
6-well Plate* ANFS-0006 3.14 17000 3000
24-well Plate* ANFS-0024 1.65 3400 1000
96-well Plate* ANFS-0096 0.33 360 200
*ANSI/SLAS compliant. All numbers approximate and subject to revision.

Biomimetic nanoscale surface topography imitates the aligned structure of the native extracellular matrix

NanoSurface nanopatterned culture surfaces provide a cellular microenvironment that mimics the aligned architecture of the native extracellular matrix, promoting cellular structural and functional development.

View our Cytostretchers.

NanoSurface
The underlying matrix of the native myocardium has an aligned architecture (scale bar 10µm)
NanoSurface
NanoSurface CoverglassNanoSurface Coverglassarrow11A) NanoSurface Coverglass

NanoSurface coverglass features the same polymeric nanoscale topography found in NanoSurface culture dishes and plates.

Unpatterned control coverglass is also available: fabricated with same polymer and process as NanoSurface coverglass, without nanoscale topography.

  • 0.16 to 0.19 mm thick
  • 25mm diameter circular coverglass
  • Polymeric surface
  • Either NanoSurface parallel surface topography OR unpatterned control surface
64820-01 25 mm circular NanoSurface coverglass
No 1.5 thickness
800:800:600 nm surface topography
50/pk 500.00 Add to Cart
64820-02 25 mm circular NanoSurface coverglass
No 1.5 thickness
Unpatterned "flat" control surface
50/pk 500.00 Add to Cart
35mm NanoSurface Dish35mm NanoSurface Disharrow11B) 35 mm NanoSurface Dish

35mm glass-bottom dish with a polymeric nanopatterned surface topography.

  • Glass-Bottom
  • NanoSurface topography
  • 0.16 to 0.19 mm thick
  • 20mm Glass Diameter
  • Gamma Sterilized
  • Cell Culture Treated
64821-01 35 mm NanoSurface Dish
800:800:600 nm surface topography
10/pk 150.00 Add to Cart
64821-02 35 mm NanoSurface Dish
800:800:600 nm surface topography
100/pk 1,100.00 Add to Cart
64821-03 35 mm NanoSurface Dish
Unpatterned "flat" control surface
10/pk 150.00 Add to Cart
64821-04 35 mm NanoSurface Dish
Unpatterned "flat" control surface
100/pk 1,100.00 Add to Cart
6-Well NanoSurface Plate24-Well NanoSurface Plate96-Well NanoSurface Platearrow11C) NanoSurface Plates

6, 24 or 96-well glass-bottom plates with a polymeric nanopatterned surface topography.

  • Glass-Bottom
  • NanoSurface topography
  • 0.16 to 0.19 mm thick
  • 20mm Glass Diameter
  • Gamma Sterilized
  • Cell Culture Treated
64822-01 6-Well NanoSurface Plate each 100.00 Add to Cart
64822-02 6-Well NanoSurface Plate 10/pk 720.00 Add to Cart
64822-03 24-Well NanoSurface Plate each 150.00 Add to Cart
64822-04 24-Well NanoSurface Plate 10/pk 1,080.00 Add to Cart
64822-05 96-Well NanoSurface Plate each 225.00 Add to Cart
64822-06 96-Well NanoSurface Plate 10/pk 1,620.00 Add to Cart

Wells, Optical Bottom Plates, Cell Culture Inserts arrow13arrow13