Microarraying

ONCYTE® Film Slides

Nitrocellulose-coated (NC) slides for protein microarrays, cell lysate arrays, tissue printing and other applications

ONCYTE® is a three-dimensional microarray comprised of a combination of nitrocellulose polymer and proprietary chemistry that provides increased protein affinity and binding. It is also provides enhanced fluorescent chemiluminescent, radiographic, colormetric and visual signal compared to glass or other coated glass surfaces.

We provide nitrocellulose-coated products that includes anything from standards 25 mm x 75 mm glass microscope slides to SBS microtiter plate substrates in standard and custom formats.

Nitrocellulose film excels in various applications including

• Protein Microarrays
• Immuno-arrays
• Analyte Microarrays
• Cell Lysate Arrays
• Antibody Arrays
• Tissue Printing

Our nitrocellulose film surfaces are manufactured and quality tested to the most exacting standards. To provide an optimal balance between binding capacity and fluorescence sensitivity, we offer two variants of nitrocellulose coatings.

The ONCYTE® AVID™ nitrocellulose-coated substrates are for applications that require the highest possible protein binding affinity and capacity. Therefore AVID™ slides are characterized by unparalleled protein binding capacity and affinity, making them ideal for colometric and infrared applications that require extremely high levels of binding.

The ONCYTE® NOVA™ nitrocellulose-coated substrates are recommended for applications that require the greatest fluorescent sensitivity but can accept reduced binding capacity. Therefore NOVA™ slides extremely low background fluorescence and an elegant, clean surface make them the most sensitive slides available for fluorescence – dependent arrays.

For Glass Slides (25 x 75 x 1mm)

EMS #	Type	Pack	Price	Select	Specifications
70335-50A	AVID™	20	500.00	Cart
70335-50N	NOVA™	20	500.00	Cart
1 - 20 x 51mm NC pad per slide
70335-51A	AVID™	20	565.00	Cart
70335-51N	NOVA™	20	568.00	Cart
1 - 20 x 60mm NC pad per slide
70335-52A	AVID™	20	565.00	Cart
70335-52N	NOVA™	20	568.00	Cart
1 - 21 x 71mm NC pad per slide
70335-53A	AVID™	20	602.00	Cart
70335-53N	NOVA™	20	602.00	Cart
2 - 20 x 20mm NC pad per slide
70335-54A	AVID™	20	602.00	Cart
70335-54N	NOVA™	20	602.00	Cart
8 - 6 x 6mm NC pad per slide
70335-55A	AVID™	20	660.00	Cart
70335-55N	NOVA™	20	660.00	Cart
12 - 6 x 6mm NC pad per slide
70335-56A	AVID™	20	760.00	Cart
70335-56N	NOVA™	20	760.00	Cart
16 - 6 x 6mm NC pad per slide
70335-57A	AVID™	20	760.00	Cart
70335-57N	NOVA™	20	760.00	Cart
16 - 6.5 x 6.5mm NC pad per slide
70335-58A	AVID™	20	840.00	Cart
70335-58N	NOVA™	20	840.00	Cart
64 - 2.5 x 2.5mm NC pad per slide
70335-59A	AVID™	20	630.00	Cart
70335-59N	NOVA™	20	619.00	Cart
1 - 15 x 69mm NC pad per slide
70335-60A	AVID™	20	619.00	Cart
70335-60N	NOVA™	20	619.00	Cart
2 - 25 x 32mm NC pad per slide
70335-61A	AVID™	20	619.00	Cart
70335-61N	NOVA™	20	619.00	Cart
4 - 15 x 15mm NC pad per slide
70335-62A	AVID™	20	715.00	Cart
70335-62N	NOVA™	20	760.00	Cart
8 - 6 x 15mm NC pad per slide
70335-63A	AVID™	20	715.00	Cart
70335-63N	NOVA™	20	765.00	Cart
8 - 6 x 20mm NC pad per slide

For SBS Compliant Microtiter Plates with Glass Substrate

EMS #	Type	Pack	Price
70335-70A	AVID™	each	200.00	Cart
70335-70N	NOVA™	each	200.00	Cart
96 NC Round Pads 6mm diameter
70335-71A	AVID™	each	202.00	Cart
70335-71N	NOVA™	each	204.00	Cart
96 NC Square Pads 6 x 6mm
70335-72A	AVID™	each	228.00	Cart
70335-72N	NOVA™	each	228.00	Cart
384 NC Square Pads 2.5 x 2.5mm

ONCYTE® Barcode Formats

EMS #	Description	Pack	Price	Select
70335-BF1	20 x 2.5 mm	each	1.50	Add to Cart
70335-BF2	20 x 7 mm	each	1.50	Add to Cart
70335-BF2	22 x 14mm	each	1.50	Add to Cart

References

1. High Definition Cell Analysis IN Situ Using Microporous Films. Cel Vision 2:499 – 599, 1995.
2. Cytometrically Coherent Transfer of Receptor Proteins on Microporous Membranes. BioTechnique 20:641-650. 1991.

Film Well™

Slide based low volume, removable chambers for protein binding

A combination of our SecureSeal™ chambers and ONCYTE® nitrocellulose film coated slides that provide an easily removable, low volume chamber and the protein binding benefits of a nitrocellulose coating.

ONCYTE® is a 3-dimensional microarray substrate bound to a standard 25mm x 75mm glass slide. A combination of nitrocellulose polymer and proprietary chemistry maximize protein binding and stability. The film is compatible with virtually all detection systems, including fluorescent, chemiluminescent, radiographic and colorimetric.

Please refer to the SecureSeal™ chambers (Click here for details) and ONCYTE® Film Slides (Click here for details) pages for pricing and ordering information.

ProPlate™ MicroArray Slide System

Integration of microplate and biochip technologies

ProPlate™ integrates microscope slide based array technology with automated microtiter plate processing. Up to 64 individual arrays can be assayed per slide. Four slides fit into a tray, producing a modular plate with a standard microtiter plate footprint and well spacing allowing automated robotic processing of up to 256 arrays.

• Up to 64 leak-proof wells on any slide surface without the use of adhesive
• Microtiter plate well spacing facilitates use with multichannel pipettes
• 7 x 7 mm square well are ideal for high content arrays
• 5 – 350 µl well volumes accommodate small reagent and large wash volumes
• Available in Chip Chip™ compatible formats.

Ordering

SBS Compliant Tray Sets

Each ProPlate Tray Set includes: 3 to 4 Slide modules, 8 Snap clips, 1 Tray and Cover, 10 seal strips and one applicator.
Tray conforms to SBS standards for footprint (81.7mm width x 123.7mm length) and well spacing.

Slide Modules

EMS #	Clip	Qty	Price	Select	Specifications
63484-21	-	10	202.00	Cart
63484-31	Delrin	2	61.00	Cart
63484-41	SS	2	61.00	Cart
1 Well ProPlate Module 16.5 x 70 mm Well
63484-22	-	10	202.00	Cart
63484-32	Delrin	2	61.00	Cart
63484-42	SS	2	61.00	Cart
2 Well ProPlate Module 16.5 x 34 mm Well
63484-23	-	10	202.00	Cart
63484-33	Delrin	2	61.00	Cart
63484-43	SS	2	61.00	Cart
3 Well ProPlate Module 18 x 20 mm Well
63484-24	-	10	204.00	Cart
63484-34	Delrin	2	61.00	Cart
63484-44	SS	2	82.00	Cart
4 Well ProPlate Module 16 x 16 mm Well
63484-25	-	10	204.00	Cart
63484-35	Delrin	2	61.00	Cart
63484-45	SS	2	61.00	Cart
8 Well ProPlate Module 7 x 16 mm Well
63484-26	-	10	204.00	Cart
63484-36	Delrin	2	61.00	Cart
63484-46	SS	2	61.00	Cart
16 Well ProPlate Module 6 mm Diameter Well 10 per pack
63484-27	-	10	206.00	Cart
63484-37	Delrin	2	61.00	Cart
63484-47	SS	2	63.00	Cart
16 Well ProPlate Module- 7 x 7 mm Well
63484-28	-	10	240.00	Cart
63484-38	Delrin	2	82.00	Cart
63484-48	SS	2	61.00	Cart
64 Well ProPlate Module 3.5 x 3.5 mm Well

ProPlate Silicone Gasket only

Accessories

EMS #	Qty	Price
63484-80	50	56.00	Cart	Seal Strips (50) and Seal Strip Applicator
63484-81	20	52.00	Cart	ProPlate Delrin®, Non-Numbered Snap Clips
63484-82	20	52.00	Cart	ProPlate Stainless Steel Spring Clips
63484-83	each	86.00	Cart	ProPlate Tray and Cover for Snap Clip Modules Holds 4 Slide Modules
63484-84	each	86.00	Cart	ProPlate Tray and Cover for Spring Clip Modules Holds 3 Slide Modules

ProPlate Microtiter Plate (MP)

Patented ProPlate™ technology for high throughput robotic processing of microarrays

Bottomless SBS compliant microtiter plate superstructures were developed for use with robotic processors. TheSuperstructures come in a permanently affixed well format – the ProPlate MP™, or a removable well format – the FlexPlate MP™.

The ProPlate MP™ SBS compliant microtiter plate features a SecureSeal™ Plus adhesive bonding system applied to a bottomless 96-round well, black, polystyrene microtiter plate. Simply peel off the protective polyliner and apply large format array for automated processing.

The proprietary adhesive forms a water-tight seal between a microarray substrate and the bottomless microtiter plate. Grace Bio Labs offers plate with SBS standard spacing in both 96-well (shown) and 384-well formats.

Key product features include

• Guaranteed flat SBS compliant microtiter plate format which enables robotic processing of assays
• Water-tight seal between superstructure and substrate
• Compatible with glass or plastic substrates.
• Available in 96 round or square or 384 square well format
• Available as a complete microtiter plate with microcellulose film coated substrate, uncoated substrate or as an adhesive superstructure alone.
• Plates are available with SBS standard spacing in both 96-well and 384-well formats.

Ordering

SBS Compliant Adhesive and Non-Adhesive Microtiter Plates

SBS Compliant Microtiter Plates with ONCYTE® Nitrocellulose Coated Substrates

FlexWell™ Incubation Chambers

FlexWell™ removable incubation chambers for microarrays using ONCYTE® slides

Features

• Silicone FlexWell™ forms well on slides using a clean release adhesive to isolate up to 16 specimens per slide
• Slides and FlexWell™ fit into a reusable slide tray organizing wells into the footprint of a SBS compliant microtiter plate
• Gasket may be sealed for mixing and to prevent evaporation
• Available in eight-well and 16-well formats

Ordering

EMS #	Qty	Price	Select	Description
63485-50	10	112.00	Cart
FlexWell 8 – 6.5 x 6.5 mm Wells 3.2mm Depth 25 x 75 mm OD Clear Silicone Adhesive one Side
63485-51	10	162.00	Cart
FlexWell 16 – 6.5 x 6.5 mm Wells 3.2mm Depth 25 x 75 mm OD Clear Silicone Adhesive one Side
63485-52	10	88.00	Cart
FlexWell 64 – 3.5 x 3.5 mm Wells 1.8mm Depth 25 x 75 mm OD Clear Silicone Adhesive one Side
63485-53	50	56.00	Cart
Seal Strips 24 mm x 77 mm Seal Area
63485-54	each	40.00	Cart
4 Slide Tray Holds up to 4 Slides Tray Measures 75.9 x 127.6 mm OD
63485-55	5	246.00	Cart
FlexPlate 96 Square Well 7.25 x 7.25 mm 9 mm Center-to-Center Well Spacing 4 mm Depth 110 x 75 mm OD Clear Silicone Adhesive on Side
63485-56	each	92.00	Cart
With Black Polystyrene Frame

References

Microarraying Products References

Chambers for Microarrays

Versatile Hybridization Chambers for Genomic and Proteomic Arrays

• Versatile sizes and styles
• Ideal for high throughput screening of genomic and protein arrays on microscope slides
• Designed to minimize reagent volume, eliminate evaporation and promote reagent mixing for uniform hybridization
• Materials are nuclease free and temperature rersistant

HybriSlip™ Hybridization Coverslips (Click here for details)

• Specially selected hydrophobic plastic covers do not bind probes to surfaces like glass coverslips
• HybriSlips™ are lighter weight than glass, minimizing friction and facilitating uniform reagent distribution
• HybriSlips™ are ready-to-use without pretreatment
• Working surfaces are protected with clean release liners to prevent contaminations
• HybriSlips™ remain flat and will not curl, even at high temperatures

HybriWell™ Hybridization System (Click here for details)

• HybriWell™ seals securely to a microscope slide surface in seconds
• Access ports in the chamber surface allow the addition of reagents
• Ports are easily sealed using seal tabs provided with each order
• Sealed chambers are water tight
• The disposable chamber removes cleanly and easily even after heating.

SecureSeal™ Hybridization Chambers (Click here for details)

• Gasketed chambers provide optimum surface volume fluid dynamics for hybridization assays.
• Designed to minimize friction especially when active mixing is employed during assay
• Partially filled chambers may be sealed and rotated continuously for superior mixing

FlexWell™ (Click here for details)

• Silicone gasket forms wells on slides using clean release adhesive to isolate up to 16 specimens per slide
• Slides fit into a reusable slide tray organizing wells into the footprint of a SMS compliant micrototer plate
• Gasket may be sealed for mixing and to prevent evaporation. Available 8-wells and 16-wells.

Note: HybriWell™ and SecureSeal™ are recommended for most protein and nucleic acid assays. If you are using CY5® or Alexa Fluor® 647 direct-labeled DNA probes, please refer to our new “fluorescent friendly” chambers for DNA microarrays or click here for details.

Hybridization and Incubation