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Electron Microscopy Sciences

Technical Data Sheets

Giemsa Thick Film Stain

EMS Catalog #: 26154-Series


None. Films are prepared by spreading 3 – 5 drops of blood on a scrupulously clean slide to form a circle of about 15 mm diameter: the films are air dried by keeping in a horizontal position for 18 – 24 hours at room temperature, protected from dust and insects.


  1. Stain 40-120 minutes in Giemsa Thick Film Staining Solution (#26154-01). *
    1. To prepare Giemsa Thick Film Staining Solution from Giemsa Stock Solution (#26154-03), mix before use:
                  Giemsa Stock Solution (#26154-03) - 1 ml
                  Phosphate Buffer (#26154-02) - 2 ml
                  Distilled Water - 47 ml
  2. Wash 5-10 minutes in distilled water or Phosphate Buffer (#26154-02)
  3. Dry in the air and examine.

Stain Results:

Malarial Parasites Clear Red Chromatin
Cytoplasm Clear Blue
Red Corpuscles Not seen due to lacking of hemoglobin during drying


Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 126

Lillie, R. D. Histologic Technic and Practical Histochemistry, 3rd ed., Mcgraw-Hill, New York, 1965.

Barber, M.A., and Komp, H.W., Pub. Health Rep., 44:2330-41, 1929

Product Information

Giemsa Thick Film Stain