Weigert's Resorcin Fuchsin
EMS Catalog #: 26370-Series
Fixation:
Any common reagent may be used.
Sections:
Paraffin at 6µm.
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- Stain in Resorcin-Fuchsin Solution (#26370-01) for 1 – 12 hours depending upon the depth of stain required for the elastic fibers. (Nuclei may be stained in Weigert Iron Hematoxylin Working Solution* prior to the Resorcin Fuchsin rather than alter as is described here).
- * To prepare working solution, (prepare fresh just before use) mix equal parts of Weigert’s Iron Hematoxylin A (#26370-03) and Weigert’s Iron Hematoxylin B (#26370-04).
- Clear the stain in 95% Alcohol. Acid Alcohol may be used to differentiate any slide that has stained diffusely wash in tap water.
- Stain slides in Weigert’s Iron Hematoxylin Working Solution (see above) and then counterstain in Van Gieson’s Solution (#26370-02)
- Rinse in distilled water to remove excess Van Gieson’s Stain from slides.
- Differentiate and hydrate the slides in two changes each of 95% Alcohol and absolute alcohol. Clear the slides in two changes of Xylene.
- Mount.
Stain Results:
Elastic Tissues |
Dark Blue |
Nuclei |
Dark Blue |
Collagen |
Red Pink |
Miscellaneous Tissue Elements |
Yellow |
References:
Mallory, F.B.: Pathological Technique; New York, Hafner Publishing Co., c. 1961, p. 168
Clark, G.: Staining Procedures: Baltimore: Williams and Wikins Co., 3rd ed., c. 1973, p. 60
Luna, L.G.: AFIP Histologic Staining Methods: New York: McGraw-Hill Book Co., 3rd ed., c. 1968, p.80.
Product Information
Weigert's Resorcin Fuchsin