Vogt's Method for Nerve Cell Products
EMS Catalog #26671-Series
Fixation
Formalin, 10% Neutral Buffered (#15740).
Sections
Paraffin @ 6 microns
Staining
- Deparaffinize and carry through absolute alcohol, two changes.
- Let stand in absolute alcohol for two hours.
- Stain in Cresyl Violet Acetate Working Solution (#26671-1A) for 40 – 60 minutes.
- Differentiate rapidly in 95% alcohol, then dehydrate in absolute alcohol, and clear in xylene, two changes each.
- Mount with Permount (#17986-01)
Staining Results
Nissl Substances |
Intense Purple |
Nuclei |
Purple |
Background |
Clear |
References
AFIP Manual of Histologic Staining Technique; ed. L. Luna., 3rd ed. McGraw-Hill Publications, c. 1968, p. 212.
