Image Gallery: UranyLess
VLPs (Norovirus virus-like particles)
Negative staining with Uranyless examined by TEM
- Photo credit: Dr. André Diessner, Icon Genetics GmbH - D-06120 Halle/Saale Germany
- Contrast the UranyLess monitoring Lead Citrate
Plant Tissue
Preparation of the sample using the following protocol:
- Glutaraldehyde Fixation Classic - Osmium - Included in Epon
- Contrast the UranyLess monitoring Lead Citrate
Trematodes
Preparation of the sample using the following protocol:
- Classic Glutaraldehyde Fixation, Osmium, Inclusion in Spurr Resin
- Contrast the UranyLess monitoring Lead Citrate
Sacculina Crustaceans (Small Parasitic Crustacean)
Preparation of the sample using the following protocol:
- Classic Gutaraldehyde Fixation, Osmium, Epoxy Inclusion
- Fine Cups - Contrast to the Aqueous UranyLess to 60°C on a Hotplate without Lead Citrate Post Coloring
Reconstituted Epidermis
Preparation of the sample using the following protocol:
- Fixing Classic Glutaraldehyde, Osmium, Epon / Araldite
- Cutting Ultra-Thin, Double UranyLess Contrast and Lead Citrate
PLC contrast Leica EM Stain
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - contrast Uranyless Lead -Citrate
Chantal Cazevieille CRIC / IURC INSERM Montpellier tested aqueous UranyLess in the Leica brand grid contrast controller on different tissues, Drosophila heart atrium, retina, cochlea and ileum (Gut). The tissues were fixed according to the standard protocol 2.5% Glutaraldehyde in PHEM buffer, the post fixation in 0.5% osmium in 0.8% potassium ferrocyanide in RT for 2 hours. The sections are collected on single-hole or 200 mesh grids.
The treatment of the grids is UranyLess 7mn lead citrate followed 7 minutes.
We present here some images made by Hitachi transmission electron microscope with a digital camera AMT.
You will notice that the combined action of potassium ferrocyanide and UranyLess reveal a marked way the cyto-membranes in the ileum.
Liver Mouse and Gerbil Sahara
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess Lead - Citrate
Muscle - Nerve - Mice
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute
Intestine
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess Lead -Citrate
Ovarian Follicle Mouse
We tested the UranyLess on various accessory cells constituting the mouse ovarian follicle.
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess Lead -Citrate
Mouse Kidney
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute
Adrenal Gland Gerbil Sahara
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Mouse Cardiac Muscle
Preparation of the sample using the following protocol:
- Classic - Fixing Glutaraldehyde, Osmium, Epon Ultrafine
- Cups, Double UranyLess Contrast and Lead Citrate
Drosophila Larva
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Parsley and Rosebush
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Culture Cells
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Phage T6
Preparation of the sample using the following protocol:
- Staggering Phage T6 on a G300-Cu grid Covered with a Carbon Formvar Film. Ionization 1 minute
Bacteria E. Coli
Negative Staining for 2 Minutes UranyLess Bacteria Like E. Coli (Adherent and Invasive (ACSI) LF82) Which Have Pili and Flagella.
Yeasts
Preparation of the sample using the following protocol:
- Classic Fixation Glutaraldehyde - Osmium - Included in Epon
- Contrast the UranyLess monitoring Lead Citrate
Cross-Sectional Bacteria
Preparation of the sample using the following protocol:
- Fixing Classic Glutaraldehyde, Osmium, EPON
- Cutting Ultrafine, Double Contrast UranyLess and Lead Citrate.
Polymersomes
UranyLess was tested in comparison with uranyl acetate, which is at acidic pH 4 (seems to disrupt the organization of the molecular structure) in compraison also the comments by the technique Cryo SEM (scanning electron microscopy).
The chemical structure is organized as follows:
All photos above taken on Transmission Electron Microscope