VLPs (Norovirus virus-like particles)

Negative staining with Uranyless examined by TEM

• Photo credit: Dr. André Diessner, Icon Genetics GmbH - D-06120 Halle/Saale Germany
• Contrast the UranyLess monitoring Lead Citrate

Plant Tissue

Preparation of the sample using the following protocol:

• Glutaraldehyde Fixation Classic - Osmium - Included in Epon
• Contrast the UranyLess monitoring Lead Citrate

Trematodes

Preparation of the sample using the following protocol:

• Classic Glutaraldehyde Fixation, Osmium, Inclusion in Spurr Resin
• Contrast the UranyLess monitoring Lead Citrate

Sacculina Crustaceans (Small Parasitic Crustacean)

Preparation of the sample using the following protocol:

• Classic Gutaraldehyde Fixation, Osmium, Epoxy Inclusion
• Fine Cups - Contrast to the Aqueous UranyLess to 60°C on a Hotplate without Lead Citrate Post Coloring

Reconstituted Epidermis

Preparation of the sample using the following protocol:

• Fixing Classic Glutaraldehyde, Osmium, Epon / Araldite
• Cutting Ultra-Thin, Double UranyLess Contrast and Lead Citrate

PLC contrast Leica EM Stain

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - contrast Uranyless Lead -Citrate

Chantal Cazevieille CRIC / IURC INSERM Montpellier tested aqueous UranyLess in the Leica brand grid contrast controller on different tissues, Drosophila heart atrium, retina, cochlea and ileum (Gut). The tissues were fixed according to the standard protocol 2.5% Glutaraldehyde in PHEM buffer, the post fixation in 0.5% osmium in 0.8% potassium ferrocyanide in RT for 2 hours. The sections are collected on single-hole or 200 mesh grids.

The treatment of the grids is UranyLess 7mn lead citrate followed 7 minutes.

We present here some images made by Hitachi transmission electron microscope with a digital camera AMT.

You will notice that the combined action of potassium ferrocyanide and UranyLess reveal a marked way the cyto-membranes in the ileum.

Liver Mouse and Gerbil Sahara

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess Lead - Citrate

Muscle - Nerve - Mice

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute

Intestine

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess Lead -Citrate

Ovarian Follicle Mouse

We tested the UranyLess on various accessory cells constituting the mouse ovarian follicle.

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess Lead -Citrate

Mouse Kidney

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute

Adrenal Gland Gerbil Sahara

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess lead -citrate

Mouse Cardiac Muscle

Preparation of the sample using the following protocol:

• Classic - Fixing Glutaraldehyde, Osmium, Epon Ultrafine
• Cups, Double UranyLess Contrast and Lead Citrate

Drosophila Larva

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess lead -citrate

Parsley and Rosebush

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess lead -citrate

Culture Cells

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess lead -citrate

Phage T6

Preparation of the sample using the following protocol:

• Staggering Phage T6 on a G300-Cu grid Covered with a Carbon Formvar Film. Ionization 1 minute

Bacteria E. Coli

Negative Staining for 2 Minutes UranyLess Bacteria Like E. Coli (Adherent and Invasive (ACSI) LF82) Which Have Pili and Flagella.

Yeasts

Preparation of the sample using the following protocol:

• Classic Fixation Glutaraldehyde - Osmium - Included in Epon
• Contrast the UranyLess monitoring Lead Citrate

Cross-Sectional Bacteria

Preparation of the sample using the following protocol:

• Fixing Classic Glutaraldehyde, Osmium, EPON
• Cutting Ultrafine, Double Contrast UranyLess and Lead Citrate.

Polymersomes

UranyLess was tested in comparison with uranyl acetate, which is at acidic pH 4 (seems to disrupt the organization of the molecular structure) in compraison also the comments by the technique Cryo SEM (scanning electron microscopy).

The chemical structure is organized as follows: