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Electron Microscopy Sciences

Embedding Media Kits

arrow14Araldite to Styrene

Technical Tips: General Tips for Embedding Media Kits

*Please note that wherever DMP-30 is listed as the accelerator in our embedding media kits you have the option to use BDMA in place of it. BDMA is less viscous, has a longer shelf life, and offers better penetration (it should be noted, however, that in order to achieve the optimum results when using BDMA in place of DMP-30 you must vary the amount. DMP-30: 1.5-2%; BDMA: 2.5-3%).

technical data sheetarrow12Araldite 502

Luft's Formula. Polymerization takes place overnight so blocks can be sectioned the next day. J. Biochem. Biophy. Cytol. 9, 409 (1961).

RT 13900 Araldite 502 Kit consists of: kit 40.00 Add to Cart
450 ml Araldite 502 msds
450 ml DDSA; Specially Distilled msds
*50 ml DMP-30 msds

technical data sheetarrow12Araldite 6005

Also known as American Araldite. The blocks are slightly harder than those produced by Araldite 502.

RT 13920 Araldite 6005 consists of: kit 43.00 Add to Cart
450 ml Araldite 6005 msds
450 ml DDSA; Specially Distilled msds
100 ml BDMA msds
225 ml DBP msds

technical data sheetarrow12Araldite, EMbed 812 (Epon-812)

Mollenhauer Epon-Araldite Formula. For hard blocks and high image contrast. Blocks are easily sectioned. Stain Tech., 39,11 (1964).

RT 13940 Araldite-EMbed (Mollenhauer’s Kit): kit 62.00 Add to Cart
450 ml EMbed-812 msds
450 ml Araldite 502 msds
450 ml DDSA; Specially Distilled msds
*50 ml DMP-30 msds

DER 732-336technical data sheetarrow12DER 332-732

Three different mixtures: one for relatively soft blocks, another for harder blocks for collagenous tissue, and a tougher one if required.
Lockwood, W.R. Anatomical Record, 150, 129 (1964)

RT 14000 Kit consists of:
2x225 ml DER 332
225 ml DER 732
450 ml DDSA; Specially Distilled
*50 ml DMP-30
kit 49.00 Add to Cart

msdsmsdstechnical data sheetarrow12Durcupan, Water Soluble

A water soluble embedding medium for EM based on an aliphatic polyepoxide.
A Fluka A.G., Buchs. Switzerland-Registered Trademark.

RT 14020 Kit 260g for a minimum of 10 embeddings kit 325.00 Add to Cart

technical data sheetarrow12Durcupan ACM, Epoxy Resin

An aromatic polyepoxide; a colorless resin of relatively low viscosity, with virtually no shrinkage.

RT 14040 Kit 1200 ml: kit 425.00 Add to Cart
Single Component A msds
Single Component B msds

Diethylene Glycol Distearate (DEGDS/DGD)arrow12Diethylene Glycol Distearate (DEGDS/DGD)

DGD is a removable embedding medium (for thin and thick sections) that provides embedment free sections. Good for immunolabeling and high resolution light microscopy.

Advantages:

  • Thin or thick embedment free sections can be obtained.
  • Sections are easy to cut and ribbons are easily obtainable.
  • Enhances the integrity of the specimen as it floats on the water.
  • Sections produce interference colors according to its thickness.

Ethanol and n-butyl alcohol are used as dehydrating agents.

  1. Capco, Krockmalnic, & Penman (1984). A New Method of Preparing Embedment Free Sections For TEM: Applications to the Cytoskeletal Framework and Other 3D Networks. JCB Vol. 98, pp. 1878.
  2. Valdimarsson & Huebner (1989). DEGDS as an Embedding Medium for Immunofluorescence Microscopy. Biochem. Cell Biol. 67:242.
  3. Nickerson, Krockmalnic, He, & Penman (1990). Immunolocalization in Three Dimensions: Immunogold Staining of Cytoskeletal and Nuclear Matrix Proteins in Resinless Electron Microscopy Sections. PNAS Vol. 87, pp. 2259.
RT 14010 Kit consists of:
450 ml DGD
450 ml Ethanol
450 ml n-Butyl alcohol
DGD Embedding Kit
kit 160.00 Add to Cart

technical data sheetarrow12EMbed-812/ DER 73

This mixture is less viscous than the final embedding mixture of EMbed-812.

RT 14130 Kit consists of:
2x225 ml DER 736
450 ml EMbed-812
450 ml NMA
*50 ml DMP-30
kit 62.00 Add to Cart

EMbed-812 Epon-812. technical data sheetarrow12EMbed-812

Electron Microscopy Sciences' replacement for the discontinued Epon-812. Produces the same results in terms of preservation, handling, sectioning, and staining as the old Epon-812.

  1. Hayat, M. A. Principles & Tech. for EM. Second Edition (1981)
  2. Mascorro, J.A. and Kirby, G.S. Physical characteristics of "old" Epon 812 and various Epon-like replacements. Proc. 44th Ann. Meet. Electron. Microsc. Soc. Am., pp.222-223 (1986)
  3. Mascorro, J.A. and Kirby, G.S. Novel Epoxy/Anhydride Alternatives for Biological Electron Microscopy: Physical and Performance Characteristics of EMbed 812 and LX 112 in combination with NSA/NMA/DMAE. Proc. 47th Ann. Meet. EMSA., pp. 1000-1001 (1989).
  4. Mascorro, J.A. and Kirby, G.S. Viscosity Characteristics and Hardening Rates for EMbed 812 and LX 112 with alternative Anhydride and Catalyst Choices. Proc. 49th Ann. Meet. EMSA. (1991).
RT 14120 Kit consists of: kit 59.00 Add to Cart
450 ml EMbed-812 msds
450 ml DDSA; Specially Distilled msds
450 ml NMA msds
*50 ml DMP-30 msds

msdsmsdstechnical data sheetEpo-Fixarrow12Epo-Fix

Especially formulated for Material Science

Epo-Fix is an old product that has existed only in the metallographic field until now. After extensive testing in our labs we have found that it is a perfect embedding media for a majority of material specimens.

Until now, LR White has been the most commonly used embedding media for materials due to the block hardness it offered, but there are difficulties associated with LR White. For instance, the adherance to the specimen is poor and additives are required to improve its adherance. Another major problem is the inability to orient the specimen correctly in order to cut you block afterwards. This is due to the fact that LR White needs to be oxygen starved and gelatin capsules (vertical) are recommended by the manufacturer for use. Another problem is that polymerization time in the oven can take over 24 hours.

Epo-Fix remedies all of these problems. It has very good sectioning properties, good adherance to the sample, very low viscosity, easy curing in molds (no orientation problems), and quick cure times.

Epo-Fix Technical Data:

A two component epoxy resin, characterized by negligible shrinkage during curing and good mechanical properties in its hardened state. It is particularly suited for embedding hard samples of complicated shape and for vacuum impregnation. Samples such as paper, silicon, ceramics, metals, wafers, and chips are suitable to be used with Epo-Fix. It has low viscosity, low vapour pressure, good wetting properties, and minimal shrinkage. It hardens at room temperature without significant generation of heat in approximately 8 hours (no damage from heat or pressure), and at increased temperatures, 60°C, the hardening time is reduced to 2 hours. It should be noted that if you place Epo-Fix in a desiccator during curing you will eliminate any air bubbles.

RT 1232 Epo-Fix kit includes:
1kg resin
120gm hardener
15 cups
15 stirrers
1 x 5 ml and 20 ml syringes
kit 118.00 Add to Cart

technical data sheetarrow12Gach (Glutaraldehyde/Carbohydrazide)

A water and lipid-retaining embedding polymer for EM. Excellent preservation of lipids and ultrastructure.
Hechman, C.A. et al. (1973) J. Ultrastruct. Res. 42,156.

RT 15920 Kit consists of:
10x10 ml 50% EM grade Glutaraldehyde
10x1.5g Carbohydrazide
kit 35.00 Add to Cart

arrow12technical data sheetGMA, Water Soluble

Glycol Methacrylate embedding medium provides excellent preservation (especially enzymatic autoradiographic studies). Good for E.M., L.M., and Histo. Leduc. & Bernard, (1967)
J. Ultrastr. Res., 4, 196-99.

RT 14200 Kit consists of:
450 ml GMA
225 ml n-Butylmethacrylate
15g 2,4-Dichlorobenzoyl Peroxide paste
kit 48.00 Add to Cart

technical data sheetarrow12GMA/PEG

Polyethylene Glycol - Glycol methacrylate mixture. A water miscible embedding medium for cytochemical studies and enzyme localization when light and electron microscopy need to be correlated.
Spaur, R.C. & Moriarty, G., (1977) J Histochem. Cytochem., 23:16

RT 14250 GMA-PEG Embedding Kit consists of: kit 48.00 Add to Cart
450 ml Glycol methacrylate, GMA msds
225 ml n-Butyl methacrylate msds
50 ml Ethylene glycol dimethacrylate msds
20 ml Polyethylene Glycol 400 msds
15g Benzoyl Peroxide Paste msds

arrow12Hard-Plus Resin-812

A newly formulated resin that enables the processing of specimens that have a tendency to wrinkle and be problematic.

In general, Hard Plus Resin-812 and its WPE numbers (weight per epoxide) do not vary much from batch to batch and it also has good stability under the electron beam and it stains well with heavy metals. Furthermore, the viscosity of the Hard-Plus Resin-812 is lower than the original Epon-812, making infiltration of the tissue more complete. For processing and embedding, it can be used exactly the same way as Epon-812, but normally, it is not necessary to use intermediate solvents, such as propylene oxide, hence, infiltration can immediately follow with absolute alcohol. Polymerization is normally at 60°C for 24 – 48 hours. Longer times or higher temperatures (70°C or more) will give a harder block.

For larger samples (>1x1mm3) the use of a intermediate solvent is recommended.

Hard-Plus Resin-812 is supplied only as a premix kit. All components are pre-measured, and you have to do is combine them in the right order and mix thoroughly. Each kit contains five separate mini-kits, making approximately 100g each.

RT 14115 Kit consists of:
5x50g Hard-Plus Resin-812
5x50g Hardener Hard-Plus
5x 2.5 ml Accelerator
Hard-Plus Resin-812 Kit
kit 108.00 Add to Cart

technical data sheetarrow12HPMA, Water Soluble

HPMA is a water-soluble resin used for cytochemical applications. Infiltration follows fixation of the tissue so there is no extraction of materials caused by dehydration in alcohols. Leduc, E.H. et al., (1965) J. Cell Biol., 26:137.

RT 14220 Kit consists of:
450 ml HPMA
15g Benzoyl Peroxide Paste
kit 40.00 Add to Cart

technical data sheetarrow12Immuno-Bed ™ Embedding Resin

  • A low viscosity media for light microscopy and immuno-histochemistry techniques.
  • It allows for the penetration of large immunoglobulins through the plastic sections for demonstration of antigenic sites.

J. Histochem, Cytochem., 35:595 (1987)

14260-00 Kit consists of:
750 ml Solution A
50 ml Solution B
9 g Catalyst Kit
  kit 332.00 Add to Cart
0-5°C 14260-01 Immuno-Bed Solution A only msds 750 ml 298.00 Add to Cart
RT 14260-04 Immuno-Bed Solution B only msds 50 ml 38.00 Add to Cart
RT 14260-06 Immuno-Bed Catalyst only msds 9 g 21.00 Add to Cart

arrow12JB-4 ™ Embedding Kit

A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalyzed monomer acts as a dehydration and infiltration agent; therefore, complete dehydration through 100% ethanol is not necessary (although recommended for large or dense tissues). Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4.

Features:

  • Thin sections (.5-2.0 micron) with excellent morphological structure preservation.
  • Water-clear blocks; casts in 90 minutes max. at room temp
  • Good lipid and enzyme retention when processing at low temperatures (4°C).
  • Removal of JB-4 resin prior to staining is not necessary.
  • Clearing agents such as xylene and chloroform are not needed.
  • Higher clarity and contrast than with paraffin sections.
  • Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue.
  • It produces harder blocks which is ideal for dense samples, such as bone

References:

  1. Hofman E.O. & Flores, T.R., High Resolution LM in Renal Path., Amer. J. of Clin. Path., 76:5 (1981)
  2. Beckstead, J.H., Blood, 57(6):1008 (1981)
  3. Brinn, N & Pickett, P., J. Histotech., 2(5):125-130 (1979)
  4. Block, Matthew H., et al., Lab. Med., 13(5)(1982)
  5. Helander, K.G., J. Microscopy, 132:223 (1983)
  6. Cole, M.B., J. Microscopy, 127:139 (1982)
  7. Higuchi, S., et al., Stain Tech., 54(1):5-12 (1979)
  8. Van DeVeldt, S., Am. J. Clin. Path.,73:121 (1980)
  9. Horton, W.A., J. Histochem. Cytochem., 31:417 (1983)
  10. Tacha, D.E. & Richard, T.C.., J. Histotech., 4(2):59 (1981)
RT 14270-00 JB-4 Kit Consists of:
800 ml Solution A
30 ml Solution B
12 g Catalyst
  kit 315.00 Add to Cart
RT 14270-01 JB-4 Solution A only msds 800 ml 275.00 Add to Cart
RT 14270-04 JB-4 Solution B only msds 30 ml 36.00 Add to Cart
RT 14270-06 JB-4 Catalyst msds 12 g 22.00 Add to Cart

technical data sheetarrow12 JB-4 Plus™ Embedding Kit

JB-4 Plus offers all of the same features as JB-4 except it produces less of an exothermic reaction than JB4 which is good for temperature sensitive tissues.

RT 14272-00 JB-4 Plus Kit Consists
500 ml Solution A
15 ml Solution B
8 g Benzoyl Peroxide Catalyst
  kit 294.00 Add to Cart
RT 14272-01 JB-4 Plus Solution A only msds 500 ml 227.00 Add to Cart
RT 14272-04 JB-4 Plus Solution B only msds 15 ml 78.00 Add to Cart
RT 14272-06 JB-4 Plus Catalyst msds 8 g 20.00 Add to Cart

Related Products for the JB-4

Block Holder, Chucks and Molding Cup Trays
Tissue-Tack Adhesive for JB-4
UV Mount Medium for JB-4

msdstechnical data sheetarrow12LR Gold Resin

The London Resin Company's acrylic "LR Gold" offers the histochemist and immunocytochemist the advantage of working with unfixed tissues at low temperatures, thus allowing many fixation-sensitive procedures to be carried out in 1-4 microns resin sections at -25°C using visible light. It is non-toxic and has a low viscosity.

RT 14370 Kit consists of:
500 ml LR Gold Resin
100g Polyvinylpyrolidone; 50g Benzoyl Peroxide
50g Benzil
kit 225.00 Add to Cart
RT 14371 LR Gold Resin only; 500 ml 165.00 Add to Cart

LR White Resin arrow12LR White Resin

A very low viscosity (8cps), non-toxic resin, suitable for both Light and Electron Microscopy.

LR White is a polar monomer polyhydroxylated acromatic acrylic resin. It can be cured by heat or by UV light. Sections of polymerized LR White resin are hydrophilic. This character allows immuno-cytochemistry reagents to easily penetrate into the sections and etching of the sections is not necessary (etching sections will effect delicate tissue antigens). LR White sections also show minimal non-specific staining. Its low viscosity makes it ideal for infiltrating decalcified bone and teeth as well as plant tissues. It is available in medium and hard grade.

Newman, G.R. (1987). Use and abuse of LR White. Histochem. J., 19:118
Newman, G.R. (1989). LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, Vol.2 (Hayat, M.A., Ed.). Academic Press. San Diego and London

Kit consists of: 500 ml LR White Resin, 10 ml UV accelerator

0-5°C 14380 Medium Grade (14381, 14385)   kit 140.00 Add to Cart
0-5°C 14382 Hard Grade (14383, 14385)   kit 144.00 Add to Cart
0-5°C 14381 LR White resin only; Med. Grade msds 500 ml 140.00 Add to Cart
0-5°C 14381-UC LR White resin only; Med. Grade Uncatalyzed msds 500 ml 150.00 Add to Cart
0-5°C 14383 LR White resin only; Hard Grade msds 500 ml 140.00 Add to Cart
0-5°C 14383-UC LR White resin only; Hard Grade Uncatalyzed msds 500 ml 150.00 Add to Cart
0-5°C 14385 LR White UV accelerator msds 10 ml 14.00 Add to Cart
0-5°C 14384 LR White Resin Catalyzed Soft Grade msds 500 ml 140.00 Add to Cart
0-5°C 14384-UC LR White Resin UnCatalyzed Soft Grade msds 500 ml 150.00 Add to Cart

Technical Data Sheets

Technical Tips

msdstechnical data sheetarrow12Lowicryl ResinHistocryl Resin

Histocryl acrylic resin has been specially formulated for light microscopy. It is water clear, and because it is hydrophilic (polar) it permits the use of most routine staining techniques without priorremoval or etching.

RT 14390 Kit consists of:
500 ml Histocryl Resin
50g Histocryl Catalyst (B.P.)
10 ml Histocryl Accelerator
kit 160.00 Add to Cart

Related Products

For the polymerization of our UV curing resins, see our UV Lamps and our UV Cryo

technical data sheetarrow12Lowicryl Resin

Lowicryl resins are highly cross-linked acrylate-based embedding resins, designed for use over a wide range of embedding conditions. These resins provide low viscosity at low temperatures.

The K4M kit is usable to -35°C and is a polar (hydrophilic) medium, while the HM20 kit can be used down to -70°C and is nonpolar (hydrophobic).

Lowicryl's K11M and HM23 have properties similar to K4M and HM20, but can be used at temperatures 20 degrees lower than others: K11M at -60°C, HM23 at least -80°C. All of these resins are photopolymerized by long wavelength (360nm) ultraviolet light. Since the initiation of the polymerization is largely independent of temperatures, blocks may be polymerized at the same temperatures used for infiltration. The resin may also be chemically polymerized at 60°C.

Armbruster, B.L. et al, (1982). J. Microscopy 126: 77
Carlemalm, E. et al, (1985). J. Microscopy 140:55
Hayat, M.A. (Ed.). (1989) Priciples and Techniques of Electron Microscopy, 3rd Edition. CRC Press,Inc. Boca Raton, Florida.

RT 14330 Lowicryl K4M msds
msds
msds
kit 501.00 Add to Cart
RT 14340 Lowicryl HM20 msds
msds
msds
kit 501.00 Add to Cart
RT 14350 Lowicryl K11M msds kit 501.00 Add to Cart
RT 14360 Lowicryl HM23 msds
msds
msds
msds
kit 501.00 Add to Cart

Technical Data Sheets and Newsletter

technical data sheetarrow12Lowicryl MonoStep Single Component Embedding Media

The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing.  This pre-mixed, ready to use resin, saves you time and minimizes chemical contact.  MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling.  Store at room temperature.

RT 14335 Lowicryl K4m Polar
Embedding Medium; MonoStep
msds 225g 226.00 Add to Cart
RT 14345 Lowicryl HM-20 Polar
Embedding Medium; MonoStep
msds 225g 226.00 Add to Cart

Technical Tips

Improved Polymerization of Acrylic Resins

technical data sheetLow Viscosity, as reported by Dr. Spurrarrow12Low Viscosity, as reported by Dr. Spurr

Provides excellent penetration for embedding biological tissues and rapid infiltration. Easy to prepare. The hardness is adjusted by changing the proportion of the flexibilizer (DER 736). The blocks have good trimming and sectioning qualities. Thin sections are tough under the electron beam.

Spurr, A.R., (1969) J. Ultrastruct. Res. 26, 31-42

RT 14300 Kit consists of:
450 ml NSA
225 ml ERL 4221
225 ml DER 736
25 ml DMAE
kit 64.00 Add to Cart

Technical Tip

Low Viscosity Kit Formulation with ERL 4221

msdsmsdstechnical data sheetUltraBed Kit Low Viscosity Epoxyarrow12UltraBed Kit

A Newly Introduced Low Viscosity Epoxy Embedding Kit

A Modification of the Dr. Spurr’s Formula for Biological, Material, and Mineralogical Specimens

Versatile! Easy to use! Save Time and Money with this one-step-preparation low viscosity embedding kit.

The kit consists of two components, equal amount, very low viscosity, (~65cps), in convenient dispenser top bottles. To prepare the Embedding Resin, just mix equal amounts of the two components. The resin readily infiltrates into specimen and polymerizes to a clear, hard block overnight at 60°C

  • Convenient – by weight just disperse equal amount of the A and B that you need, and mix together
  • Save time – Mixed resin can be used for both infiltration and embedding
  • Less Hazardous – All components are shipped together as Non- Hazardous substances

Kit comes with 100 ml each component A and component B

RT 14310 UltraBed Low Viscosity Kit 2x100 ml kit 214.00 Add to Cart

technical data sheetarrow12Methyl Methacrylate/Butyl Methacrylate

The mixture of Butyl and Methyl Methacrylate offers many advantages over the standard GMA and HPMA embedding mediums:

  • It can be UV cured in the cold with the addition of benzoin methyl ether, or heat cured by the addition of benzoyl peroxide.
  • The resin can be removed from the sections with a solvent (acetone) before staining.
RT 14520 225 ml Methyl Methacrylate
225 ml Butyl Methacrylate
15 gm Benzoyl Peroxide Paste
kit 37.00 Add to Cart

msdstechnical data sheetemsexclu.gif (210 bytes)arrow12Micro-Bed Resin

Micro-Bed resin is a newly developed water-soluble resin which is a mixture of acrylate and polyester resins. It is a very universal embedding medium and may be used for either light or electron microscopy.

Due to its non-crosslinking characteristics, its hydrophilic nature, and roughness of its section surface, our Micro-Bed Resin is excellent in preserving tissue structure, offers good results in immunolabeling, and stands up well to conventional stains.

In light microscopy, tissues were stained with conventional histological stains (e.g.,hematoxylin-eosin) and embedded in Micro-Bed: the results were comparable with those provided on paraffin embedded tissues. Further, polychrome silver-based and cytochemical stains (e.g.,PAS) were also feasible. In transmission electron microscopy, images as good as those obtained with similar acrylic resins, (e.g., Lowicryl K4M), were obtained.

Micro-Bed is supplied as a single component-ready to use pre-mixed resin. Micro-Bed can be polymerized in either EMS embedding capsules or gelatin capsules by either heat(55-65°C) or UV (wavelength 365nm) light at low temperatures (-10 to 20°C) for 72 hours.

Scala C. et al.(1992). Development of a new acrylic resin ideally suited for light and electron microscopy. Electron Microscopy. Volume 3.EUREM 92, Granada, Spain. pg. 271

0-5°C 14210 Micro-Bed Embedding Resin 500 ml 84.00 Add to Cart

arrow12Polyester Wax

Please see Polyester Wax

technical data sheetarrow12Quetol 651 - NSA

A water miscible epoxy resin used for both light and electron microscopy. Its low viscosity permits easy infiltration and embedding, and yields light-colored blocks.

Kushida, H. et al., (1986) Pro. 11th Int. Cong. Electron Microsc., p. 2177. Kyoto

0-5°C 14640 Kit consists of:
225 ml Quetol 651
450 ml NMA
450 ml NSA
50 ml DMP-30
kit 79.00 Add to Cart

Articles of Interest

Quetol and the Addition of Water

msdstechnical data sheetarrow12Styrene Monomer, Polyester Resin

Very low viscosity; monomeric styrene which penetrates rapidly into the tissues. It is soluble in ethanol and acetone. The infiltration and embedding can be done by styrene alone, and there is no need to dilute it with solvents. Polymerization can be done either by UV light (340-400nm) or heat (60°C) within 2-3 days without benzoyl peroxide added; 24 hours with 1% benzoyl peroxide added as a catalyst.

Delamater et al., (1971) Pro. 29th Ann. Meet. EMSA, p. 488. Claitor's Pub. Div., Baton Rouge, La.
Kushida, H. (1962b) J. Electron Microsc., 11:128
Kushida, H. (1961a) J. Electron Microsc., 10:16

RT 14650   kit 38.00 Add to Cart

Technical Tips

Rapid Techniques for Specimen Preparation

Technovit Methyl Methacrylate and Glycol Methacrylate arrow14arrow14