Electron Microscopy Sciences

               
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Histology and Light Microscopy

Electron Microscopy Sciences

arrow14 Reagents for Histology: Fixing, Dehydrating, Clearing and Staining

EMS is now offering a complete line of reagents for Histopathology: The highest quality for Fixing, Dehydrating/Clearing and Staining

arrowAcetate Buffer

RT 11482-42 Acetate Buffer, 0.2M, pH 4.2 500 ml 18.00 Add to Cart
RT 11482-50 Acetate Buffer, 0.2M, pH 5.0 500 ml 18.00 Add to Cart
RT 11482-56 Acetate Buffer, 0.2M, pH 5.6 500 ml 18.00 Add to Cart

arrowAcetic Acid, Glacial, Reagent A.C.S

C2H4O2 F.W. 60.05 CAS 64-19-7

Assay min   99.7%
RT 10040 Acetic Acid, Glacial 500 ml 58.00 Add to Cart
RT 10041 Acetic Acid, Glacial 2.5 L 115.00 Add to Cart

msdsarrowAcetic Acid, Aqueous Solution

msds

Prepared from acetic acid glacial with de-ionized water

RT 10042-02 Acetic Acid, 0.2% (v/v) Solution 18.00 500 ml Add to Cart
RT 10042-05 Acetic Acid, 0.5% (v/v) Solution 19.00 500 ml Add to Cart
RT 10042-10 Acetic Acid, 1.0% (v/v) Solution 21.00 500 ml Add to Cart
RT 10042-50 Acetic Acid, 5.0% (v/v) Solution 29.00 500 ml Add to Cart

msds arrowAcetone, Reagent, A.C.S.

CH3COCH3   F.W. 58.08   CAS #67-64-1

A.C.S. Specifications:
Assay   Min 99.5%
Aldehyde (as HCHO)   0.002%
Color   Max. APHA 10
Density (G/ml) at 25°C   Max. 0.7857
Titratable Acid   0.0003 meq/g
Titratable Base   0.0006 meq/g
Isopropyl Alchohol   0.05%
Methanol (CH3OH)   0.05%
Residue after Evaporation   0.001%
Solubilty in water   To pass test
Substances Reducing
Permanganate
  To pass test
Water   0.5%
RT 10000   1qt. (946ml) 13.00 Add to Cart
RT

 

 

  4x1 qt. 48.00 Add to Cart
RT 10012   250ml 7.00 Add to Cart
RT 10014   4x250ml 20.00 Add to Cart

arrowAcetone: Alcohol, 1:1 & Buffered Acetone

RT 10017 Acetone: Alcohol, 1:1 500 ml 20.00 Add to Cart
RT 10018 Buffered Acetone Fixative 500 ml 24.00 Add to Cart

arrowAcid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

Acid Alcohol, 0.5% HCl in 70% Alcohol 26071 msds
Acid Alcohol, 1% HCl in 70% Alcohol 26072 msds
RT 26071-01 Acid Alcohol, 0.5% HCl in 70% Alcohol 1 L 34.00 Add to Cart
RT 26071-04 Acid Alcohol, 0.5% HCl in 70% Alcohol 4x1 L 117.00 Add to Cart
RT 26072-01 Acid Alcohol, 1% HCl in 70% Alcohol 1 L 29.00 Add to Cart
RT 26072-04 Acid Alcohol, 1% HCl in 70% Alcohol 4x1 L 102.00 Add to Cart
RT 26073-01 Acid Alcohol, 1% HCl in 95% Alcohol 1 L 38.00 Add to Cart
RT 26073-04 Acid Alcohol, 1% HCl in 95% Alcohol 4x1 L 122.00 Add to Cart

msdsarrow12Ethyl Alcohol, Specially Denatured

C2H5OH   F.W. 46.07   CAS #64-17-5

Blended 95% Ethanol and 5% Methanol

Suitable for all routine histology/cytology preparations.

Specifications:
Evaporation Residue   5ppm max.
Water   0.1%
Filtered SDA-3A   95%
RT 15050   1qt (946ml) 21.00 Add to Cart
RT 15051   4x1qt 76.00 Add to Cart

msdsarrow12Ethyl Alcohol, Anhydrous

(Ethanol) - 200 Proof
CH3CH2OH   F.W. 46.07   CAS # 64-17-5

Specifications:

Specific Gravity <0.7942
Purity >99.9%
Acidity <0.002%
Water (Karl Fisher) <0.1%
Non-Volatile Matter <0.0025%
RT 15055   450ml 18.00* Add to Cart
RT 15056   4x450ml 68.00* Add to Cart
RT 15058   1 liter 32.00 Add to Cart

*Tax has already been paid

arrow12Alkaline Alcohol

RT 10380 10% NH4OH in 85.5% Alcohol 500 ml 23.00 Add to Cart
RT 10381 1.6% NaCl in 80% Alcohol 500 ml 25.00 Add to Cart
RT 10382 0.2% KOH in 80% Alcohol 500 ml 27.00 Add to Cart

msdsarrowAmmonium Hydroxide, 28% Reagent, A.C.S.

NH4OH   F.W. 35.05   CAS #1336-21-6 Specific Gravity: 0.91

Assay   28-30%

Specifications:

Residue After Ignition   0.002%
Carbon Dioxide   0.002%
Chloride   0.5 ppm
Phosphate   2 ppm
Sulfate   2 ppm
Heavy Metals   0.5 ppm
Iron   0.2 ppm
RT 10600   450ml 16.00 Add to Cart
RT 10601   4x450ml 58.00 Add to Cart

arrowAntibody Dilution Buffer with Immunoglobulin- Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2 – 8°C. Diluted antibodies can be stored at 2 – 8°C for several weeks, depending upon the stability of antibodies.

2–8°C 25886-01 Antibody Dilution Buffer, free BSA 100 ml 50.00 Add to Cart
2–8°C 25886-05  Antibody Dilution Buffer, free BSA    500 ml  100.00 Add to Cart
  1227SK Antigen Preserve Solution VII 1L 150.00 cart

arrow12B-5 Fixative

For lymph nodes.

RT 64122-05 B-5 Fixative 500 ml 64.00 Add to Cart
RT 64122-06 B-5 Fixative 1 lt 120.00 Add to Cart
RT 64122-10 B-5 Fixative 4 lt 450.00 Add to Cart

arrow12Borate Buffer

RT 11455-78 Borate Buffer, pH 7.8 30.00 500 ml Add to Cart
RT 11455-82 Borate Buffer, pH 8.2 30.00 500 ml Add to Cart
RT 11455-90 Borate Buffer, pH 9.0 30.00 500 ml Add to Cart

msdsarrow12 Bouin’s Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.

Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT 15990-01 Bouin's Fluid 1 L 41.00 Add to Cart
RT 15990-04 Bouin's Fluid 4 L 146.00 Add to Cart

Bouin’s Fluid in Pre-filled cups

RT 15990-10 Bouin's Fluid 50 x 10 ml/cs 76.00 Add to Cart
RT 15990-20 Bouin's Fluid 50 x 20 ml/cs 96.00 Add to Cart
RT 15990-30 Bouin's Fluid 50 x 30 ml/cs 43.00 Add to Cart

For other sizes and configurations of our Bouin’s Solution, click here.

msdsarrown-Butyl Alcohol, Reagent, A.C.S.

(Butanol)
CH3CH2CH2CH2OH   F.W. 74.12   CAS # 71-36-3

Specific Gravity:   0.81
Assay   99.4%
Residue after evaporation   0.005%
Titratable acid   0.0008meq/g
Butyl Ether   0.2%
Water   0.1%
RT 11920   450ml 20.00 Add to Cart
RT 11921   4x450ml 72.00 Add to Cart

arrow12Carnoy’s Fixatives

Stock solution

RT 64130-05 Carnoy’s Fluid 500ml 26.00 Add to Cart
RT 64130-50 Carnoy’s Fluid Without Chloroform 500ml 24.00 Add to Cart

arrow12Cedar Wood Oil

CAS #8000-27-9 Refractive Index @ 20°C 1.504+/-.002

For clearing.

RT 12420   100ml 30.00 Add to Cart

msdsarrow12Chloroform, Reagent, A.C.S.

CHCl3   FW 119.38   CAS #67-66-3

A.C.S. Specifications:

Color (APHA)   10
Density @ 25°C   1.471-1.476
Residue after Evaporation   0.001%
Acetone & Aldehyde (about 0.00%)   To Pass Test
Acid & Chloride   To Pass Test
Free Chlorine (Cl)   To Pass Test
Lead (Pb)   0.05ppm
Subs. darkened by H2SO4   To Pass Test
Suitability for Dithizone Test   To Pass Test
RT 12540   450ml 15.00 Add to Cart
RT 12541   4x450ml 68.00 Add to Cart

arrow12Chromic Acid, Reagent, Crystals, A.C.S.

CrO3   FW 99.99   CAS #1333-82-0

A.C.S. Specifications:

Chloride (Cl)   0.005%
Insoluble Matter   0.01%
Nitrate (NO3)   0.05%
Sodium (Na)   0.2%
Iron, Aluminum, Barium   0.03%
Sulfate (SO4)   0.05%
Assay   98%
RT 12555   100g 44.00 Add to Cart

msdsarrow12Chromic Acid 4% Aqueous Solution

RT 12557 Chromic Acid 4% Aqueous Solution 500 ml 23.00 Add to Cart
RT 12558 Chromic Acid 5% Aqueous Solution 500 ml 29.00 Add to Cart

arrow12 Clearing Solvent Xylene Substitutes

These clearing solvents offer Histology and Cytology laboratories a safer alternative to xylene.

  • Contains no xylene or toluene.
  • No citrus odor.
  • For use in manual and automated systems.
RT 64129-10 Clearing Solvent 1 gal 46.00 Add to Cart

arrow12 CytoPrep Fixative

CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.

RT 64145-01 CytoPrep Fixative 4 oz 30.00 Add to Cart
RT 64145-12 CytoPrep Fixative 12x4 oz 310.00 Add to Cart

arrow12Davidson’s Alcohol Formalin Acetic Acid Fixative (Davidson’s AFA)

A fixative solution prepared from a combination of Ethyl alcohol, Denatured (33%), 37-39% Formalin (22%), Glacial Acetic Acid (11.5%) and water, to preserve samples for Histology and/or In-Situ Hybridizations

RT 64133-10 Davidson's Fixative 1L 35.00 Add to Cart

arrow12Modified Davidson’s Fixative

(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water)

RT 64133-50 Modified Davidson's Fixative 1L 38.00 Add to Cart

RDO Rapid Decalcifierarrow12RDO Rapid Decalcifier

RDO and RDO Gold were developed with the basic premise of making your work routine quicker and easier without sacraficing the quality and integrity of your samples.

RDO and RDO Gold are remarkably effective multipurpose decacifiers that can be tailored to suit your specific lab routine.

Both RDO and RDO Gold:
  • Offer crisp nuclear staining and definition
  • Give distinguishable chromatin
  • Show great surface decalcification
  • Safe enough to be disposed down to lab sink to drain
How is RDO Gold different from the original RDO
  • RDO Gold is designated for those biopsies which contain minimal amounts of calcium
  • No filtration is required with RDO Gold
  • RDO Gold gives stellar morphological representation of bone biopsies
  • There is no folding of tissues with RDO Gold
  • RDO Gold is clear and non-precipitate
Both RDO and RDO Gold are:
  • Far Superior to other Decalcifiers
  • Great Cellular Differentiation
  • Safe – Non Toxic – Non-Corrosive
  • Dilutable
  • Microwavable
  • Superb Staining Quality
  • Biodegradable
  • Translucent
  • Available in Wide Mouth Liter Bottle
References:
  1. Gayle Callis et al. “Dacalcification of Bone: Literature Review and Practical Study of Various Decalcifying Agents, Methods, and Their Effects on Bone Histology”. The J. of Histology, Vol. 21, No. 1, March 1998.
  2. Sherley A. Powell, HT (ASCP) HTL. “Method to Facilitate Sectioning Incompletely Decalcified Bone”. Mercer University, School of Medicine. Microtime Georgia, State Newsletter.
  3. Becky Scholes, HTL, MT (ASCP).:Bone Marrow Biopsies”. H.I.S.T.O. Iowa State Newsletter.
RT 64143-01 RDO Decalcifier 1 L 20.50 cart
RT 64143-06 RDO Decalcifier 6 x 1 L/cs 102.00 cart
RT 64143-40 RDO Decalcifier 1 gal 42.00 cart
RT 64143-44 RDO Decalcifier 4 x 1 gal/cs 149.00 cart
RT 64144-01 RDO - Gold Decalcifier 1 L 34.00 cart
RT 64144-06 RDO - Gold Decalcifier 6 x 1 L/cs 180.00 cart
RT 64144-40 RDO - Gold Decalcifier 1 gal 66.00 cart
RT 64144-44 RDO - Gold Decalcifier 4 x 1 gal/cs 238.00 cart

Technical Tips and Articles

RDO Rapid Decalcifier

Animal Tissue Techniques
Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen
Bone Marrow Biopsies

Alcoholic Stain Solutionsarrow12Decalcifier

  • Ready-to-use.
  • For hard specimens.
  • Protects cellular structure.
  • Minimal interference with subsequent staining.
  • For use with human and animal calcified tissue, bone, cartilage and other hard specimens.
RT 64147-01 Decalcifier 1 L 35.00 Add to Cart
RT 64147-06 Decalcifier 6x1 L 192.00 Add to Cart
RT 64147-10 Decalcifier 10 L 176.00 Add to Cart

arrowDeionized Water, Reagent Grade A.C.S.

H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.

Color (APHA)   <+/-5
Bacteriological purity   0 CFUs/L
Residue after evaporation   10ppm
Coliform   negative
RT
22800-01 Deionized Water 1 gal 10.00 Add to Cart
RT
22800-05 Deionized Water 5 gal 42.00 Add to Cart

arrowEthyl Alcohol

Ethyl Alcohol Anhydrous Denatured, click here.
Ethyl Alcohol Anhydrous, Tax Paid, click here.

arrowEthylene Glycol Monoethyl Ether

Ethylene Glycol Monoethyl Ether, click here.

msdsarrow12Ferric Ammonium Sulfate

RT 15505-40 Ferric Ammonium Sulfate, 4% Aqueous 500 ml 29.00 Add to Cart
RT 15505-50 Ferric Ammonium Sulfate, 5% Aqueous 500 ml 31.00 Add to Cart

msdsarrow12Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl3·6H2O   F.W. 270.30   CAS #13478-10-9

Assay 97.0-102%
Insoluble Matters. 0.01%
Nitrate (about 0.001%) To Pass Test
Phosphorous Compound 0.01%
Sulfate 0.01%
Arsenic 0.002%
Copper 0.003%
Zinc 0.003%
References for use:

Membrane stains

  • Basic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol.,
    19:223
  • Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface
    electron stains. Lab. Invest., 18:63
  • Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron
    stain for surface proteins. J. Histochem. Cytochem., 22:368
  • Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and
    distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
  • Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The
    Ultrastructural localization and content of sialic acid in plasma membranes isolated from
    rat liver and hepatoma. J. Cell Sci., 2:499
  • Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron.
    Pro. 29th Ann. EMSA Meet., p.352. Claitor’s Pub. Division, Baton Rouge, LA
  • Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin,
    phospholipase C, and pH on the topography of bound positively charged colloidal
    particles. J. Cell Biol., 57:373
  • Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and
    Fontana technique. Histochemie, 26:311.
RT 15510 Ferric Chloride, Hexahydrate, Reagent, 500g 45.00 Add to Cart

arrow12Ferric Chloride 10%, Aqueous Solution

RT 15512-02 Ferric Chloride 2% Aqueous 500 ml 36.00 Add to Cart
RT 15512-10 Ferric Chloride 10% Aqueous 500 ml 36.00 Add to Cart

Alcoholic Stain Solutionsarrow12Fixative/Decalcifier

  • A solution that fixes and decalcifies in one step.
  • Prevents loss of cellular structure.
  • Enhances hematoxylin staining.
RT 64148-01 Fixative/Decalcifier 1 L 42.00 Add to Cart
RT 64148-06 Fixative/Decalcifier 6x1 L 230.00 Add to Cart

arrow12Formalde-Fresh Low Odor Solution; Histology Grade

A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.

RT 15678-01 Formalde-Fresh 4 L 74.00 Add to Cart
RT 15678-04 Formalde-Fresh 4x4 L 290.00 Add to Cart

msdsarrow12Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution

Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.

RT 15741-01 Low-Odor Formalin 10% Buffered 4 L 58.00 Add to Cart
RT 15741-04 Low-Odor Formalin 10% Buffered 4x4 L 200.00 Add to Cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C

RT 15738-01 Formalin 10% Buffered in Acetate 1L 18.00 Add to Cart
RT 15738-04 Formalin 10% Buffered in Acetate 4L 49.00 Add to Cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.f.I.P. manual of Histology.Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C

RT 15740 Formalin 10% Buffered in Phosphate 450ml 12.00 Add to Cart
RT 15740-01 Formalin 10% Buffered in Phosphate 1L 19.00 Add to Cart
RT 15740-04 Formalin 10% Buffered in Phosphate 4L 56.00 Add to cart

arrow12TissuePrep Buffered 10% Formalin

Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.

  Catalog # Cup Size Filled Pack/cs Price  
RT 15742-10 15 ml 10ml 50 77.00 Add to Cart
RT 15742-20 30 ml 20ml 50 98.00 Add to Cart
RT 15742-30 60 ml 30ml 20 44.00 Add to Cart
RT 15742-60 120 ml 60ml 20 48.00 Add to Cart

msdsarrow12Formaldehyde, Reagent, A.C.S. (Formalin)

HCHO F.W. 30.03 CAS #50-00-0

Specific Gravity   1.09

A.C.S. Specifications:

Assay   35.5 to 38.0%
Residue after ignition   0.005%
Color (A.P.H.A.)   10
Sulfate (SO4)   0.002%
Heavy Metal (as Pb)   5ppm
Titrate Acid   0.006 meq/g
Chloride (Cl)   5ppm
Iron (Fe)   5ppm
RT 15680   450ml 12.00 Add to Cart
RT 15681   4x450ml 40.00 Add to Cart
RT 15682   1qt(946ml) 22.00 Add to Cart

arrow12Formaldehyde, 37%, Microfiltered (Formalin Microfiltered)

HCHO F.W. 30.03 CAS# 50-00-0

Microfiltered with a 0.45 micron membrane; suitable as an EM fixative when a methanol free solution is not necessary.

RT 15686   10x10ml 25.00 Add to Cart

msdsAlcoholic Stain Solutionsarrow12Formaldehyde

(Paraformaldehyde) GLUTARALDEHYDE

Karnovsky’s Fixative Kit, please click here.

RT 15720   kit 17.00 Add to Cart
RT 15730   box of 5 kits 76.50 Add to Cart

msdsarrow12Formaldehyde Aqueous Solution, 16%

(Paraformaldehyde Aqueous Solution)

EM Grade

Paraformaldehyde - methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. Easy to break, prescored, 10ml ampoules sealed under inert gas, or 100ml bottles.

16% Aqueous Solution
RT 15700   10ml 5.00 Add to Cart
RT 15710   10x10ml 22.50 Add to Cart
RT 15710-S   100ml 21.00 Add to Cart
RT 15711   10 box/lot 210.00 Add to Cart
msds4% Aqueous Solution
RT 157-4   10x10ml 18.00 Add to Cart
RT 157-4-100   100ml 17.00 Add to Cart
8% Aqueous Solution
RT 157-8   10x10ml 19.50 Add to Cart
RT 157-8-100   100ml 18.00 Add to Cart
msds10% Aqueous Solution
RT 15712   10x10ml 20.50 Add to Cart
RT 15712-S   100ml 19.00 Add to Cart
msds20% Aqueous Solution
RT 15713   10x10ml 26.00 Add to Cart
RT 15713-S   100ml 24.00 Add to Cart
msds32% Aqueous Solution
RT 15714   10x10ml 31.00 Add to Cart
RT 15714-S   100ml 29.00 Add to Cart

aarrow12Formaldehyde/Zinc Fixative

Prepared: Ready-to-use.

Features:

  • Preserves fine nuclear details.
  • Good for immunostaining.
  • Contains no mercury.
  • Can be used with automated tissue processors.
  • Works well with standard and special stains because zinc is a stain enhancing mordant.
RT 15675-01 Formaldehyde/Zinc Fixative 1 L 11.00 a
RT 15675-04 Formaldehyde/Zinc Fixative 4 L 38.00 a

msdsarrow12Formic Acid 96%, Reagent, A.C.S.

HCOOH   F.W. 46.03   CAS #64-18-6
Specific Gravity: 1.22

RT 15750 Formic Acid 96% 225ml 9.00 Add to Cart
RT 15760 Formic Acid 96% 1 liter 28.00 Add to Cart

arrow12Glutaraldehyde

OCHCH2CH2CH2CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified
  • The Universal Fixative for Electron Microscopy.
  • Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
  • THE HIGHEST PURITY, free from polymers and other contaminants.
  • Each lot is tested and assayed in our lab before we ship and guarantee its purity.
  • Sealed under dry nitrogen.
  • Refrigerated prior to shipment.

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our aqueous glutaraldehyde in a wide range of percen- tages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10ml ampoules and 100ml bottle quantities for larger applications.

msds8% Aqueous Solution
0-18ºC 16000   10ml ampoule 6.00 Add to Cart
0-18ºC 16019   Box 10x2ml 16.00 Add to Cart
0-18ºC 16020   Box 10x10ml ampoules 20.00 Add to Cart
0-18ºC 16021 same as 16020 10 bxs 178.00 Add to Cart
0-18ºC 16022 same as 16020 20 bxs 348.00 Add to Cart
0-18ºC 16010   100ml bottle 17.00 Add to Cart
msds10% Aqueous Solution
0-18ºC 16100 10ml ampoule each 6.00 cart
0-18ºC 16120 Box 10x10ml ampoules box 22.00 Add to Cart
0-18ºC 16121 same as 16120 10 bxs 200.00 Add to Cart
0-18ºC 16122 same as 16120 20 bxs 390.00 Add to Cart
0-18ºC 16110 100ml bottle each 18.00 Add to Cart
msds25% Aqueous Solution
0-18ºC 16200   10ml ampoule 7.00 Add to Cart
0-18ºC 16220   Box 10x10ml ampoules 24.00 Add to Cart
0-18ºC 16221 same as 16220 10 bxs 220.00 Add to Cart
0-18ºC 16222 same as 16220 20 bxs 418.00 Add to Cart
0-18ºC 16210   100ml bottle 20.00 Add to Cart
50% Aqueous Solution
  16300 msds
  16320 msds
0-18ºC 16300   10ml ampoule 7.50 Add to Cart
0-18ºC 16320   Box 10x10ml ampoules 37.00 Add to Cart
0-18ºC 16321 same as 16320 10 bxs 335.00 Add to Cart
0-18ºC 16322 same as 16320 20 bxs 620.00 Add to Cart
0-18ºC 16310   100ml bottle 36.00 Add to Cart
msds70% Aqueous Solution
0-18ºC 16350   10ml ampoule 8.50 Add to Cart
0-18ºC 16360   box 10x10ml ampoules 50.00 Add to Cart
0-18ºC 16365   box 10x2ml ampoules 29.00 Add to Cart
Biological Grade
25% Solution 16400 msds
50% Solution 16500 msds
0-18ºC 16400 25% Solution 450ml 18.00 Add to Cart
0-18ºC 16401 25% Solution 4x450ml 68.00 Add to Cart
0-18ºC 16410 25% Solution 1 gal. 55.00 Add to Cart
0-18ºC 16411 25% Solution 4x1 gal. 210.00 Add to Cart
0-18ºC 16500 50% Solution 450ml 19.00 Add to Cart
0-18ºC 16501 50% Solution 4x450ml 73.00 Add to Cart
0-18ºC 16520 50% Solution 1 liter 23.00 Add to Cart
0-18ºC 16521 50% Solution 4x1 liter 75.00 Add to Cart

arrow12Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times

0-18ºC 16216 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10ml 36.00 Add to Cart
0-18ºC 16214 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 100ml 27.00 Add to Cart
0-18ºC 16316 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10ml 47.00 Add to Cart
0-18ºC 16314 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 100ml 37.00 Add to Cart

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

arrow12 Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792

Ordering Information: (please call for additional information)

  16530 10% in Acetone 10x10ml 75.00 Add to Cart
16531 10% in Ethanol 10x10ml 75.00 Add to Cart
16532 10% in Methanol 10x10ml 75.00 Add to Cart
16533 10% in Solvent of your choice 10x10ml 75.00 Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

msdsarrow12Glutaraldehyde Spill Control; G-gard-ER

Transforms glutaraldehyde to a nearly neutral solution and renders the solution acceptable for sewer disposal. Prepares glutaraldehyde for biodegradation. Reduces biocidal activity and odor. 65g G-gard pre-treats one gallon of 1% solution.

RT 24142-01 Glutaraldehyde Spill Control 64g 6.00 Add to Cart
RT 24142-10 Glutaraldehyde Spill Control 10x64g 25.00 Add to Cart

Ampoule Crackerarrow12Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination

60605-04 Ampoule Cracker for Ampoule Size 1 – 4 ml 500/pk 35.00 Add to Cart
60605-10 Ampoule Cracker for Ampoule Size 5 – 10 ml 500/pk 35.00 Add to Cart
60605-20 Ampoule Cracker for Ampoule Size 10 – 20 ml 500/pk 67.00 Add to Cart

Ampoule Breaker; Soft Silicon arrow12Ampoule Breaker; Soft Silicon

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600 Ampoule Breaker each 15.00 Add to Cart

msds arrow12Gold Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)

H(AuCl4)·H2O   F.W. 357.79   CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included)

RT 16580   10x1/10g 52.00/bx Add to Cart
RT 16582   10x1/2g 242.00/bx Add to Cart
RT 16583   1g 50.00/g Add to Cart
RT 16584   10x1g 480.00/bx Add to Cart

arrow12Gold Chloride Aqueous Solutions

RT 16586 Gold Chloride 0.1% Aqueous 500 ml 40.00 Add to Cart
RT 16587 Gold Chloride 0.2% Aqueous 500 ml 66.00 Add to Cart
RT 16588 Gold Chloride 0.5% Aqueous 500 ml 128.00 Add to Cart
RT 16589 Gold Chloride 1.0% Aqueous 500 ml 195.00 Add to Cart
RT 16590 Gold Chloride 10.0% Aqueous 500 ml 1,325.00 Add to Cart

arrow12Gold, Immuno, see Section 2 ImmunoGold Reagents

msdsarrow12Gum Mastic

0-4°C 16590-01 Gum Mastic, 2.5% in Alcohol 500 ml
54.00
Add to Cart
0-4°C 16591 Gum Mastic, 5% in Alcohol 500 ml 58.00 Add to Cart
0-4°C 16592 Gum Mastic, 10% in Alcohol 500 ml 60.00 Add to Cart
0-4°C 16595 Gum Mastic, 10% in Isopropanol 500 ml

60.00

Add to Cart

msdsarrow12Hexamethylenetetramine, A.C.S. Reagent, Granular

(Urotropine; Methenamine)

C6N4   F.W. 140.19   CAS #100-97-0

Assay:   99.0-100.5%

Specifications:

Residue after Ignition   0.1%
Chloride   0.014%
Sulfate   To Pass Test
Heavy Metals.   0.001%

Used in silver stains.

Swift, J.A. (1968) Microsc. Soc. 88, 449.

RT 16710   50g 11.00 Add to Cart
RT 16710-25*   25g 6.00 Add to Cart
RT 16712 Methynamine, 3% Aqueous 500 ml 25.00 Add to Cart

msds arrow12Hexylene Glycol, Reagent, A.C.S.

(2-Methyl-2,4-pentanediol)

(CH3)2C(OH)CH2CH(OH)CH3   F.W. 118.18   CAS #107-41-5

Specific Gravity @25°C 0.923

A.C.S. Specifications:

Assay   99%
IR Spectrum   To Pass Test
Refractive Index @20°C   0.921-0.923

Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.

RT 16740   450ml 23.00 Add to Cart
RT 16750   4x450ml 88.00 Add to Cart

Reagents: HISTOCHOICE to Zinc/Formalin arrow12arrow12

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