Acetate Buffer

RT	11482-42	Acetate Buffer, 0.2M, pH 4.2	500 ml	18.00	Add to Cart
RT	11482-50	Acetate Buffer, 0.2M, pH 5.0	500 ml	18.00	Add to Cart
RT	11482-56	Acetate Buffer, 0.2M, pH 5.6	500 ml	18.00	Add to Cart

Acetic Acid, Glacial, Reagent A.C.S

C2H4O2 F.W. 60.05 CAS 64-19-7

Assay min

99.7%

RT	10040	Acetic Acid, Glacial	500 ml	58.00	Add to Cart
RT	10041	Acetic Acid, Glacial	2.5 L	115.00	Add to Cart

Acetic Acid, Aqueous Solution

Prepared from acetic acid glacial with de-ionized water

RT	10042-02	Acetic Acid, 0.2% (v/v) Solution	18.00	500 ml	Add to Cart
RT	10042-05	Acetic Acid, 0.5% (v/v) Solution	19.00	500 ml	Add to Cart
RT	10042-10	Acetic Acid, 1.0% (v/v) Solution	21.00	500 ml	Add to Cart
RT	10042-50	Acetic Acid, 5.0% (v/v) Solution	29.00	500 ml	Add to Cart

Acetone, Reagent, A.C.S.

CH₃COCH₃   F.W. 58.08   CAS #67-64-1

A.C.S. Specifications:

Assay		Min 99.5%
Aldehyde (as HCHO)		0.002%
Color		Max. APHA 10
Density (G/ml) at 25°C		Max. 0.7857
Titratable Acid		0.0003 meq/g
Titratable Base		0.0006 meq/g
Isopropyl Alchohol		0.05%
Methanol (CH3OH)		0.05%
Residue after Evaporation		0.001%
Solubilty in water		To pass test
Substances Reducing Permanganate		To pass test
Water		0.5%

RT	10000		1qt. (946ml)	13.00	Add to Cart
RT			4x1 qt.	48.00	Add to Cart
RT	10012		250ml	7.00	Add to Cart
RT	10014		4x250ml	20.00	Add to Cart

Acetone: Alcohol, 1:1 & Buffered Acetone

RT	10017	Acetone: Alcohol, 1:1	500 ml	20.00	Add to Cart
RT	10018	Buffered Acetone Fixative	500 ml	24.00	Add to Cart

Acid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

Acid Alcohol, 0.5% HCl in 70% Alcohol	26071
Acid Alcohol, 1% HCl in 70% Alcohol	26072

RT	26071-01	Acid Alcohol, 0.5% HCl in 70% Alcohol	1 L	34.00	Add to Cart
RT	26071-04	Acid Alcohol, 0.5% HCl in 70% Alcohol	4x1 L	117.00	Add to Cart
RT	26072-01	Acid Alcohol, 1% HCl in 70% Alcohol	1 L	29.00	Add to Cart
RT	26072-04	Acid Alcohol, 1% HCl in 70% Alcohol	4x1 L	102.00	Add to Cart
RT	26073-01	Acid Alcohol, 1% HCl in 95% Alcohol	1 L	38.00	Add to Cart
RT	26073-04	Acid Alcohol, 1% HCl in 95% Alcohol	4x1 L	122.00	Add to Cart

Ethyl Alcohol, Specially Denatured

C₂H₅OH   F.W. 46.07   CAS #64-17-5

Blended 95% Ethanol and 5% Methanol

Suitable for all routine histology/cytology preparations.

Specifications:

Evaporation Residue		5ppm max.
Water		0.1%
Filtered SDA-3A		95%

RT	15050		1qt (946ml)	21.00	Add to Cart
RT	15051		4x1qt	76.00	Add to Cart

Ethyl Alcohol, Anhydrous

(Ethanol) - 200 Proof
CH₃CH₂OH   F.W. 46.07   CAS # 64-17-5

Specifications:

Specific Gravity	<0.7942
Purity	>99.9%
Acidity	<0.002%
Water (Karl Fisher)	<0.1%
Non-Volatile Matter	<0.0025%

RT	15055		450ml	18.00*	Add to Cart
RT	15056		4x450ml	68.00*	Add to Cart
RT	15058		1 liter	32.00	Add to Cart

*Tax has already been paid

Alkaline Alcohol

RT	10380	10% NH4OH in 85.5% Alcohol	500 ml	23.00	Add to Cart
RT	10381	1.6% NaCl in 80% Alcohol	500 ml	25.00	Add to Cart
RT	10382	0.2% KOH in 80% Alcohol	500 ml	27.00	Add to Cart

Ammonium Hydroxide, 28% Reagent, A.C.S.

NH₄OH   F.W. 35.05   CAS #1336-21-6 Specific Gravity: 0.91

Assay

28-30%

Specifications:

Residue After Ignition		0.002%
Carbon Dioxide		0.002%
Chloride		0.5 ppm
Phosphate		2 ppm
Sulfate		2 ppm
Heavy Metals		0.5 ppm
Iron		0.2 ppm

RT	10600		450ml	13.00	Add to Cart
RT	10601		4x450ml	47.00	Add to Cart

Antibody Dilution Buffer with Immunoglobulin- Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2 – 8°C. Diluted antibodies can be stored at 2 – 8°C for several weeks, depending upon the stability of antibodies.

2–8°C	25886-01	Antibody Dilution Buffer, free BSA	100 ml	40.00	Add to Cart
2–8°C	25886-05	Antibody Dilution Buffer, free BSA	500 ml	99.00	Add to Cart

B-5 Fixative

For lymph nodes.

RT	64122-05	B-5 Fixative	500 ml	64.00	Add to Cart
RT	64122-06	B-5 Fixative	1 lt	120.00	Add to Cart
RT	64122-10	B-5 Fixative	4 lt	450.00	Add to Cart

Borate Buffer

RT	11455-78	Borate Buffer, pH 7.8	30.00	500 ml	Add to Cart
RT	11455-82	Borate Buffer, pH 8.2	30.00	500 ml	Add to Cart
RT	11455-90	Borate Buffer, pH 9.0	30.00	500 ml	Add to Cart

Bouin’s Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.

Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT	15990-01	Bouin's Fluid	1 L	41.00	Add to Cart
RT	15990-04	Bouin's Fluid	4 L	146.00	Add to Cart

Bouin’s Fluid in Pre-filled cups

RT	15990-10	Bouin's Fluid	50 x 10 ml/cs	76.00	Add to Cart
RT	15990-20	Bouin's Fluid	50 x 20 ml/cs	96.00	Add to Cart
RT	15990-30	Bouin's Fluid	50 x 30 ml/cs	43.00	Add to Cart

For other sizes and configurations of our Bouin’s Solution, click here.

n-Butyl Alcohol, Reagent, A.C.S.

(Butanol)
CH₃CH₂CH₂CH₂OH   F.W. 74.12   CAS # 71-36-3

Specific Gravity:		0.81
Assay		99.4%
Residue after evaporation		0.005%
Titratable acid		0.0008meq/g
Butyl Ether		0.2%
Water		0.1%

RT	11920		450ml	20.00	Add to Cart
RT	11921		4x450ml	72.00	Add to Cart

Carnoy’s Fixatives

Stock solution

RT	64130-05	Carnoy’s Fluid	500ml	26.00	Add to Cart
RT	64130-50	Carnoy’s Fluid Without Chloroform	500ml	24.00	Add to Cart

Cedar Wood Oil

CAS #8000-27-9 Refractive Index @ 20°C 1.504+/-.002

For clearing.

RT

12420

100ml

30.00

Add to Cart

Chloroform, Reagent, A.C.S.

CHCl₃   FW 119.38   CAS #67-66-3

A.C.S. Specifications:

Color (APHA)		10
Density @ 25°C		1.471-1.476
Residue after Evaporation		0.001%
Acetone & Aldehyde (about 0.00%)		To Pass Test
Acid & Chloride		To Pass Test
Free Chlorine (Cl)		To Pass Test
Lead (Pb)		0.05ppm
Subs. darkened by H2SO4		To Pass Test
Suitability for Dithizone Test		To Pass Test

RT	12540		450ml	15.00	Add to Cart
RT	12541		4x450ml	68.00	Add to Cart

Chromic Acid, Reagent, Crystals, A.C.S.

CrO₃   FW 99.99   CAS #1333-82-0

A.C.S. Specifications:

Chloride (Cl)		0.005%
Insoluble Matter		0.01%
Nitrate (NO3)		0.05%
Sodium (Na)		0.2%
Iron, Aluminum, Barium		0.03%
Sulfate (SO4)		0.05%
Assay		98%

RT

12555

100g

40.00

Add to Cart

Chromic Acid 4% Aqueous Solution

RT	12557	Chromic Acid 4% Aqueous Solution	500 ml	23.00	Add to Cart
RT	12558	Chromic Acid 5% Aqueous Solution	500 ml	29.00	Add to Cart

Clearing Solvent Xylene Substitutes

These clearing solvents offer Histology and Cytology laboratories a safer alternative to xylene.

• Contains no xylene or toluene.
• No citrus odor.
• For use in manual and automated systems.

RT

64129-10

Clearing Solvent

1 gal

40.00

Add to Cart

CytoPrep Fixative

CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.

RT	64145-01	CytoPrep Fixative	4 oz	20.00	Add to Cart
RT	64145-12	CytoPrep Fixative	12x4 oz	230.00	Add to Cart

Davidson’s Alcohol Formalin Acetic Acid Fixative (Davidson’s AFA)

A fixative solution prepared from a combination of Ethyl alcohol, Denatured (33%), 37-39% Formalin (22%), Glacial Acetic Acid (11.5%) and water, to preserve samples for Histology and/or In-Situ Hybridizations

RT

64133-10

Davidson's Fixative

1L

35.00

Add to Cart

Modified Davidson’s Fixative

(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water)

RT

64133-50

Modified Davidson's Fixative

1L

38.00

Add to Cart

RDO Rapid Decalcifier

RDO and RDO Gold were developed with the basic premise of making your work routine quicker and easier without sacraficing the quality and integrity of your samples.

RDO and RDO Gold are remarkably effective multipurpose decacifiers that can be tailored to suit your specific lab routine.

Both RDO and RDO Gold:

• Offer crisp nuclear staining and definition
• Give distinguishable chromatin
• Show great surface decalcification
• Safe enough to be disposed down to lab sink to drain

How is RDO Gold different from the original RDO

• RDO Gold is designated for those biopsies which contain minimal amounts of calcium
• No filtration is required with RDO Gold
• RDO Gold gives stellar morphological representation of bone biopsies
• There is no folding of tissues with RDO Gold
• RDO Gold is clear and non-precipitate

Both RDO and RDO Gold are:

• Far Superior to other Decalcifiers
• Great Cellular Differentiation
• Safe – Non Toxic – Non-Corrosive
• Dilutable
• Microwavable
• Superb Staining Quality
• Biodegradable
• Translucent
• Available in Wide Mouth Liter Bottle

References:

1. Gayle Callis et al. “Dacalcification of Bone: Literature Review and Practical Study of Various Decalcifying Agents, Methods, and Their Effects on Bone Histology”. The J. of Histology, Vol. 21, No. 1, March 1998.
2. Sherley A. Powell, HT (ASCP) HTL. “Method to Facilitate Sectioning Incompletely Decalcified Bone”. Mercer University, School of Medicine. Microtime Georgia, State Newsletter.
3. Becky Scholes, HTL, MT (ASCP).:Bone Marrow Biopsies”. H.I.S.T.O. Iowa State Newsletter.

RT	64143-01	RDO Decalcifier	1 L	19.00
RT	64143-06	RDO Decalcifier	6 x 1 L/cs	95.00
RT	64143-40	RDO Decalcifier	1 gal	40.00
RT	64143-44	RDO Decalcifier	4 x 1 gal/cs	142.00
RT	64144-01	RDO - Gold Decalcifier	1 L	34.00
RT	64144-06	RDO - Gold Decalcifier	6 x 1 L/cs	180.00
RT	64144-40	RDO - Gold Decalcifier	1 gal	66.00
RT	64144-44	RDO - Gold Decalcifier	4 x 1 gal/cs	238.00

Technical Tips and Articles

RDO Rapid Decalcifier

Animal Tissue Techniques
Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen
Bone Marrow Biopsies

Decalcifier

• Ready-to-use.
• For hard specimens.
• Protects cellular structure.
• Minimal interference with subsequent staining.
• For use with human and animal calcified tissue, bone, cartilage and other hard specimens.

RT	64147-01	Decalcifier	1 L	30.00	Add to Cart
RT	64147-06	Decalcifier	6x1 L	175.00	Add to Cart
RT	64147-10	Decalcifier	10 L	150.00	Add to Cart

Deionized Water, Reagent Grade A.C.S.

H₂O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.

Color (APHA)		<+/-5
Bacteriological purity		0 CFUs/L
Residue after evaporation		10ppm
Coliform		negative

RT	22800-01	Deionized Water	1 gal	10.00	Add to Cart
RT	22800-05	Deionized Water	5 gal	42.00	Add to Cart

Ethyl Alcohol

Ethyl Alcohol Anhydrous Denatured, click here.
Ethyl Alcohol Anhydrous, Tax Paid, click here.

Ethylene Glycol Monoethyl Ether

Ethylene Glycol Monoethyl Ether, click here.

Ferric Ammonium Sulfate

RT	15505-40	Ferric Ammonium Sulfate, 4% Aqueous	500 ml	29.00	Add to Cart
RT	15505-50	Ferric Ammonium Sulfate, 5% Aqueous	500 ml	31.00	Add to Cart

Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl₃·6H₂O   F.W. 270.30   CAS #13478-10-9

Assay	97.0-102%
Insoluble Matters.	0.01%
Nitrate (about 0.001%)	To Pass Test
Phosphorous Compound	0.01%
Sulfate	0.01%
Arsenic	0.002%
Copper	0.003%
Zinc	0.003%

References for use:

Membrane stains

• Basic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol.,
  19:223
• Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface
  electron stains. Lab. Invest., 18:63
• Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron
  stain for surface proteins. J. Histochem. Cytochem., 22:368
• Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and
  distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
• Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The
  Ultrastructural localization and content of sialic acid in plasma membranes isolated from
  rat liver and hepatoma. J. Cell Sci., 2:499
• Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron.
  Pro. 29th Ann. EMSA Meet., p.352. Claitor’s Pub. Division, Baton Rouge, LA
• Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin,
  phospholipase C, and pH on the topography of bound positively charged colloidal
  particles. J. Cell Biol., 57:373
• Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and
  Fontana technique. Histochemie, 26:311.

RT

15510

Ferric Chloride, Hexahydrate, Reagent,

500g

45.00

Add to Cart

Ferric Chloride 10%, Aqueous Solution

RT	15512-02	Ferric Chloride 2% Aqueous	500 ml	36.00	Add to Cart
RT	15512-10	Ferric Chloride 10% Aqueous	500 ml	36.00	Add to Cart

Fixative/Decalcifier

• A solution that fixes and decalcifies in one step.
• Prevents loss of cellular structure.
• Enhances hematoxylin staining.

RT	64148-01	Fixative/Decalcifier	1 L	40.00	Add to Cart
RT	64148-06	Fixative/Decalcifier	6x1 L	230.00	Add to Cart

Formalde-Fresh Low Odor Solution; Histology Grade

A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.

RT	15678-01	Formalde-Fresh	4 L	67.00	Add to Cart
RT	15678-04	Formalde-Fresh	4x4 L	260.00	Add to Cart

Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution

Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.

RT	15741-01	Low-Odor Formalin 10% Buffered	4 L	53.00	Add to Cart
RT	15741-04	Low-Odor Formalin 10% Buffered	4x4 L	176.00	Add to Cart

Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C

RT	15738-01	Formalin 10% Buffered in Acetate	1L	16.00	Add to Cart
RT	15738-04	Formalin 10% Buffered in Acetate	4L	47.00	Add to Cart

Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.f.I.P. manual of Histology.Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C

RT	15740	Formalin 10% Buffered in Phosphate	450ml	10.50	Add to Cart
RT	15740-01	Formalin 10% Buffered in Phosphate	1L	17.25	Add to Cart
RT	15740-04	Formalin 10% Buffered in Phosphate	4L	52.00	Add to cart

TissuePrep Buffered 10% Formalin

Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.

	Catalog #	Cup Size	Filled	Pack/cs	Price
RT	15742-10	15 ml	10ml	50