Fluoro-Gel (A Direct Substitute for BioMedaTM Gel-Mount)

Fluoro-Gel is a water based mounting medium designed for the permanent mounting of fluorescent stained tissues, which may either be damaged or soluble in organic solvents, such as xylene or toluene. This unique formula prevents rapid photo-bleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent Protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4ºC in the dark.

Application: Immunofluorescence, confocal microscope.

For our original formula:

0-5ºC	17985-10	Fluoro-Gel, (with Tris Buffer)	20 ml	40.00
0-5ºC	17985-11	Fluoro-Gel, (with Tris Buffer)	100 ml	150.00

For a more stable pH:

0-5ºC	17985-30	Fluoro-Gel, (with TES Buffer)	20 ml	40.00
0-5ºC	17985-31	Fluoro-Gel, (with TES Buffer)	100 ml	150.00
0-5ºC	17985-40	Fluoro-Gel, (with PIPE Buffer)	20 ml	40.00
0-5ºC	17985-41	Fluoro-Gel, (with PIPE Buffer)	100 ml	150.00

Fluoro-Gel II Mounting Medium

Fluoro-Gel II is similar to Fluoro-Gel, but Fluoro-Gel II contains DAPI (4, 6-diamino-2-phenylindole), which is a counter stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing blue fluorescence. RNA is also stained with DAPI.

Application: Immunofluorescence, confocal microscopy.

	17985-50	Fluoro-Gel II	20 ml	60.00
	17985-51	Fluoro-Gel II	100 ml	250.00

Fluoro-Gel III Mounting Medium

Fluoro-Gel III Mounting Medium is similar to Fluoro-Gel, however, Fluoro-Gel III with the addition of PI (Propidium Iodide) which is a counterstain for DNA, makes this a unique mounting medium, which prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4ºC in the dark. This medium does not contain Phynylenediamine, which destroys immunofluoresccence of Cy dyes, RP-E, PC, and APC. This product is to be used in in situ hybridization techniques or other method where fluorescence of DNA staining is required. PI excites at 535 nm, emits at 615 nm, producing a red fluorescence. RNA is also stained with PI.

Application: Immunofluorescence, confocal microscope

	17985-60	Fluoro-Gel III	20 ml	60.00
	17985-61	Fluoro-Gel III	100 ml	250.00

Fluoro-Gel with Anti-Fading Mounting Medium

Fluoro-Gel Mounting Medium with a strong anti-fading agent 1,4-phenylenediamine is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula preserves rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 388, Alexa Fluoro 594 tetramethyly rhodamine, and Redox. The fluorescence is retained during prolong storage at 4°C in the dark. This medium contains Phynylenediamine and is not suitable for immunofluorescence of Cy dyes, Phycoerythrin (R-PR) phycocyanin  (PC), and allophycocyanin (APC).

Application: Immuno-fluorescence, confocol microscopy.

Reagent: Prepared from TRIS buffer. Ready-to-use mounting medium with dark coffee color, this is normal, and does not interfere with immuno-fluorescence

Storage: 2 – 8°C is recommended. Protect from light. DO NOT FREEZE.

	Cat. #	Description	Qty	Price
2–8°C	17983-20	Fluoro-Gel with Anti-Fading Agent	20 ml	$42.00	Add to Cart
2–8°C	17983-100	Fluoro-Gel with Anti-Fading Agent	100 ml	$170.00	Add to Cart

Clear-Mount  (A Direct Substitute for BioMeda™ Crystal Mount)

Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents.  Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.

This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H & E staining.

Application: Mounting of Immunohisto slides.

For our original formula:

	17985-12	Clear-Mount, (with Tris Buffer)	30 ml	52.00
	17985-15	Clear-Mount, (with Tris Buffer)	250 ml	280.00

For a more stable pH:

	17985-16	Clear-Mount, (with PIPE Buffer)	30 ml	52.00
	17985-17	Clear-Mount, (with PIPE Buffer)	100 ml	125.00

*The names BioMeda™ Gel Mount and Crystal Mount are all owned by BioMeda Corporation

In-Situ Mount (A Direct Substitute for BioMeda™ Situ Mount)

In-Situ mounting medium is a unique mounting medium designed for permanent preservation of tissue sections and cell smear with alkaline phosphatase chromogens like NBT/BCIP and red stain.

Application: Mounting tissues and cell smears.

0-5ºC

17988-30

In-Situ Mounting Medium

30 ml

54.00

*The name BioMeda™ Situ Mount is owned by BioMeda Corporation

Limonene-Mount  (A Direct Substitutefor BioMeda™ Clarion Mount)

This mounting medium is made with limonene, a natural product from orange peels. It preserves  tissues and cell smears that can be dehydrated with organic solvents in immunohistochemistry, e.g. DAB and DAB with nickel or cobalt. Our Limonene-Mount also works well with alkaline phosphatase chromogens, and organic solvent resistant Supper Fast Red. It is also a good choice for mounting H & E stained slides.

Application: Mounting tissues and cell smears.

	17987-01	Limonene-Mount	100 ml	178.00
	17987-06	Limonene-Mount	6x100 ml	960.00
	17987-25	Limonene-Mount	250 ml	210.00

*The name BioMeda™ Clarion is owned by BioMeda Corporation.

Buffers

Universal Immunoassay Buffer™, 10X
Universal Antibody Dilution Buffer™
Antibody Dilution Buffer with Immunoglobulin- Free BSA

Biomount™

Some mounting media oxidize rapidly upon exposure to air, forming carboxyl groups. This can be more pronounced when sections have been cleared in a solution containing aldehyde. It is sometimes observed that the immunogold/silver signal fades after a few weeks, or even in a shorter time, from sections that have been mounted with these media under cover slips. The silver is still present, but has formed translucent silver carboxylate salts. Visibility can be retrieved by removing the cover slip and washing in xylene and then immersing the slide in photographic developer, but this is a tedious procedure.

BIOMOUNT is a specially formulated mounting medium that reduces fading of immunogold/silver signals in sections on glass slides. It is suitable for resins as well as wax embedded sections of tissue.

RT

17894

BioMount

100ml

40.00

CITIFLUOR™ - Antifadent Mountant Solutions

A mounting medium for
immunofluorescence, which overcomes the problems of fading.

This mounting media is designed to reduce the fading of the fluorescence of dyes used for labeling biological specimens.

In the technique of fluorescence microscopy, the fluorescence is stimulated by high intensity UV and/or visible light. Absorption of the light populates an excited state of the dye (usually a singlet state) and this leads to fluorescence. However, the excited state of the dye may undergo chemical reaction, which leads to destruction as evidenced by the fading of the fluorescence and consequent loss of the image. Citifluor™ mounting media is designed to solve this problem.

Selection of the appropriate product:

There is no universal antifadent due to the fact that biological material is so varied in nature that the fluorescence fading of fluorochromic conjugates is a complex process.

The general anti-fadents are AF1, AF2, and AF3. The AF87 is a specialist immersion oil-based antifadent.

AF1 (Glycerol-phosphate buffered solution containing an additive for use with labeled tissue sections). Should be used if you normally examine specimens in glycol solution. If you normally use saline solution with your specimens.

AF2 (Glycerol solution containing additives for use with labeled tissue sections) is simply a glycerol solution of the antifadent.

AF3 (Phosphate-buffered saline solution containing additives for examination of whole cell) is appropriate.

AF87, the antifadent, is contained in non-fluorescent immersion oil. It is designed for those requiring work at high magnification. It is useful for preventing the fading of such dyes as DAPI, which have the tendency to disperse in glycerol based mountant solutions.

CFPVOH is an aqueous solution of poly(vinyl alcohol) for use as a solid CFPVOH is an aqueous solution of poly (vinyl alcohol) for use as a solid mountant. Apply a few drops of the solution to your sample, and allow drying to give a clear film.

AF100 is a solution of antifadents to be used with CFPVOH when fading of fluorescence in the poly(vinyl alcohol) is a problem. To prepare a CFPVOH solution containing AF100, add 0.1ml of Af100 to 0.9ml of CFPVOH. If more or less of the solid mountant solution is required, mix the reagent in the appropriate ratio. Once these solutions have been made up, they should be used within a period of days since the solution deteriorates on storage.

All of these products have a long shelf life, a minimum of 2 years. The solution can be stored at room temp-erature. With LD50’s >2000mg/kg (in rat), this product exhibits no toxicological problems.

RT	17970-25	CitiFluor™ AF1	25 ml	86.00
RT	17970-100	CitiFluor™ AF1	100 ml	198.00
RT	17971-25	CitiFluor™ AF2	25 ml	86.00
RT	17971-100	CitiFluor™ AF2	100 ml	198.00
RT	17972-25	CitiFluor™ AF3	25 ml	86.00
RT	17972-100	CitiFluor™ AF3	100 ml	198.00
RT	17976-10	CitiFluor™ AF87	10 ml	68.00
RT	17976-25	CitiFluor™ AF87	25 ml	115.00
RT	17978-35	Set of CitiFluor™ CFPVOH and AF100*	25 ml + 5 ml	98.00

* Only available when ordering with CFPVOH

• Citifluor AF1, AF2 & AF3
• Citifluor AF87
• Citifluor CFPVOH AND AF10

Mounting Medium for all Laser Scanning and Conventional Microscopes

A Revolutionary New Mounting Medium which helps to control the effects of Refraction.

THESE MOUNTANT SOLUTIONS:

• Have a refractive index of 1.52 to match the refractive index of your slides and coverslips
• Are simple to use – Just apply the solution to the specimen followed by a cover slip
• The solution may be kept at room temperature – comes with dropper tip to aid in dispensing
• All mountants are water miscible (aqueous glycerol based), and therefore the specimen does not need to be dehydrated prior to the addition of the mounting solution.
• Has a pH compatible with the specimens: CFM-1 has a pH of ~6.5, and CFM-2 has a pH of ~8.5
• Contains an antifadent to help reduce the bleaching of the fluorochromes (CFM-1 Plus Antifadent)
• May be used as a immersion oils in addition to being a mountant solution and since they are water-based, they facilitate cleaning the microscope optics.

CFM-1 and CFM-1 Plus Antifadent

This glycerol-phosphate buffered saline based mountant solution has been specially formulated so as to have a refractive index of ~1.52 @ room temperature. The addition of antifadent is to retard the bleaching of fluorochromes. Its refractive index is similar to glass; therefore effects of refraction on image quality are minimized. The CFM-1 plus antifadent
will be particularly useful for high magnification work, where immersion oils are use to minimize distortion of the image due to the refraction of the viewing light and where bleaching of the flurochrome occurs.

The solutions should also be very valuable for laser scanning microscopy where, three-dimensional imaging of specimens is carried out e.g. with confocal fluorescence microscopy where integrity of the image has to be maintained.

Both solutions are medium viscosity, and water-clear.

CFM-1 Plus Antifadent have a pH of ~10

CFM-1 has a pH of ~6.5

CFM-2 Mountant

This glycerol-tris-buffered based mountant solution has been specially formulated to have a refractive index of ~1.52 (at room temperature) i.e. a refractive index similar to glass so that the effects of refraction on image quality are minimized. This mountant solution may also be used as an immersion oil. The CFM solutions will be particularly useful for high magnification work where immersion oils are used to minimize distortion of the image due to refraction of the viewing light. The solutions should also be particularly valuable for lasing scanning microscopy where three-dimensional imaging of specimens is carried out e.g. with confocal microscopy.

The solution has a medium viscosity, and it is water-clear, and has a pH of ~8.5

RT	17980-10	CFM-1 Mountant Solution	10 ml	50.00
RT	17980-25	CFM-1 Mountant Solution	25 ml	94.00
RT	17981-10	CFM-1 Plus Mountant Solution	10 ml	60.00
RT	17981-25	CFM-1 Plus Mountant Solution	25 ml	120.00
RT	17982-10	CFM-2 Mountant Solution	10 ml	60.00
RT	17982-25	CFM-2 Mountant Solution	25 ml	120.00

Cytoseal MountingMedium

Premium quality; formulated from the highest quality advanced acrylic resin. It will not become brittle and crack, nor will it discolor or yellow with age. CytoSeal dries rapidly, allowing examination soon after application. It can be used with oil immersion objectives and in fluorescent procedures. Slides will not stick together and the medium will not "cold flow" to the edge of the slide during long term storage. CytoSeal can be dissolved in toluene or xylene. An antioxidant has been added to the formulation to prevent fading of slides.

CytoSeal comes in a Controlled Drop Dispenser, with varying viscosities.

* CytoSeal 60, which has a viscosity of 60cps, is intended primarily for use with cover glass.

* CytoSeal 280, which has a viscosity of 280cps is intended for use where minimal spread of medium is required.

RT	18006	CytoSeal 60	4oz	22.00
RT	18007	CytoSeal 60	16oz	52.00
RT	18008	CytoSeal 280	4oz	21.00

DPX Mountant for Microscopy

A mixture of Distyrene, a plasticizer, and xylene. A colorless synthetic resin mounting media which replaces Xylene-Balsam. It preserves the stain and dries quickly.

RT	13510		100ml	54.00
RT	13512		500ml	102.00

DEPEX Mounting Media

A colorless, neutral, xylene miscible mountant which is suitable for preparations where Canada Balsam is usually used.

RT	13514		100ml	61.00
RT	13515		500ml	225.00

Technical Tip

DEPEX: An improved Mounting Media

Entellan® New! Improved Formula

Product Details

RT	14800		100ml	50.00
RT	14802		500ml	188.00

Eukitt™ Mounting Medium

Product Details

RT	15320	Eukitt™	100ml	66.00
RT	15322	Eukitt™	500ml	179.00

Fluoromount-G™Slide MountingMedium

A special mounting media for immunofluorescent staining procedures. It is a water soluble, non-fluorescing medium which is well suited for use when the staining procedure has an aqueous final step. It contains 10% polyvinyl alcohol in phosphate buffered glycerol with 0.1% sodium azide as a preservative. One bottle is sufficient to mount up to 500 slides.

0-5ºC

17984-25

Fluoromount-G™

25 ml

59.00

Glycerin Jelly

For the mounting of hydrated sections.

RT	17998-10	Glycerin Jelly	100 ml	26.00
RT	17998-50	Glycerin Jelly	500ml	116.00

Histomount

A mixture of xylene - mixed isomers and non hazardous ingredients to offer a very good synthetic mountant. Once dried the refractive index is 1.58.

RT	17995-01	Histomount	100 ml	32.00
RT	17995-05	Histomount	450 ml	54.00

Liquid Coverglass; SHUR/Mount™

Shur/Mount is a liquid coverglass and mounting medium. Its low viscosity minimizes bubble formation under the coverglass. An antioxidant present in the medium prevents fading of the stain even over years of storage. A UV absorbent present in our Shur/Mount prevents the yellowing of the resin.

Available in three types:

• A toluene-based acrylic resin; rapid drying. It is used for mounting and preservation of microscope slide specimens, and is ideal for manual coverslipping.
• A xylene-based formula which is used for automatic coverslipping instruments.
• A unique water based mounting media for use in all Immuno procedures.

RT	17990-01	Shur/Mount™ - Toluene Based	4 oz.	34.00
RT	17990-12	Shur/Mount™ - Toluene Based	12x4 oz./cs	384.00
RT	17991-01	Shur/Mount™ - Xylene Based	16 oz.	64.00
RT	17991-06	Shur/Mount™ - Xylene Based	4x16 oz./cs	284.00
RT	17992-01	Shur/Mount™ - Water Based	20 ml	84.00
RT	17992-04	Shur/Mount™ - Water Based	4x20 ml	316.00

Cargille Meltmounts™

Cargille Meltmount™(optical quality) is a series of mounting media which are specifically formulated for use in microscope slide mounting and in other optical coupling applications.

Meltmount™ Features:

• Instant thermal mountants that require "no-oven-time".
• 100% Usable; contains no solvents.
• Reversible, thermally, for particle retrieval or re-orientation
• Soluble in toluene for special techniques or clean-up.
• Fluid at 65°C, a temperature chosen because it makes a permanent mount and protects the majority of specimens from thermal changes.
• PCB-Free.
• It is a direct replacement for old mounting mediums (Canada Balsam, Aroclor 5442, Naphrax...)

Quick-Stick™ Form

Melt Mount is also available in a Quick-Stick™ form which can be used to make permanent microscope slide mounts quickly. These Melt Mount sticks can be applied to a slide on a hotplate. As soon as the specimen and coverglass are positioned and the slide is cooled you have a permanent prepared slide that can be reversed by reheating, if you should wish to retrieve a particular particle. Each Quick-Stick™ weighs approximately 2/3 oz. (18g).

1. Meltmount™ 1.539

Has a refractive index (nD @25°C) of 1.539 and an Abbe V dispersion of 45. Its optically similar to Canada Balsam and therefore ideal for mounting many biological specimens but without the long drying time of Canada Balsam.

RT	17994-10	Meltmount 1.539	1 oz.	64.00
RT	17994-11	Quick-Stick™ Meltmount 1.539	each	57.00

2. Meltmount™ for Chrysotile Asbestos

It has dispersion characteristics making it appropriate for mounting chrysotile asbestos.

RT

17994-20

Meltmount for Chrysotile

1 oz.

105.00

3. Meltmount™ 1.582

It has a refractive index (nD @25°C) of 1.582 and an Abbe V dispersion of 33. Its optical clarity makes it the preferred choice for minimum visible absorption.

RT	17994-30	Meltmount 1.582	1 oz.	61.00
RT	17994-31	Meltmount 1.582 Quick-Stick™	each	57.00

4. Meltmount™ 1.605

It has a refractive index (nD @25°C) of 1.605 and an Abbe V dispersion of 30, making it appropriate for mounting Asbestiform Tremolite, Anthophyllite, and Actinolite.

RT	17994-40	Meltmount 1.605	1 oz.	61.00
RT	17994-41	Meltmount 1.605 Quick-Stick™	each	57.00

5. Meltmount™ 1.662

It has a refractive index (nD @25°C) of 1.662 and an Abbe V dispersion of 26, optically similar to Aroclor 5442 but is PCB-free.

RT	17994-50	Meltmount 1.662	1 oz.	61.00
RT	17994-51	Meltmount 1.662 Quick-Stick™	each	57.00

6. Meltmount™ 1.680

It has a refractive index (nD @25°C) of 1.680 and an Abbe V dispersion of 25, making it appropriate for mounting Amosite and Crocidolite asbestos.

RT	17994-60	Meltmount 1.680	1 oz.	61.00
RT	17994-61	Meltmount 1.680 Quick-Stick™	each	57.00

7. Meltmount™ 1.704

It has a refractive index (nD @25°C) of 1.704 and an Abbe V dispersion of 24, similar to Naphrax.

RT	17994-70	Meltmount 1.704	1 oz.	61.00
RT	17994-71	Meltmount 1.704 Quick-Stick™	each	57.00

Mount Quick, Solvent/Water Base

Mounting media for cover glass.  It comes in a convenient dispensing tube or bottle.   Solvent based which is soluble in xylene and has a 10 minute dry time.  Its viscosity is 33 cps ar 25º.

RT	18000	Mount Quick, Solvent Base	30 cc	16.00
RT	18002	Mount Quick, Water Base	30 cc	23.00

Permount™* Mounting Medium

A toluene-based synthetic resin mounting medium. The right choice for both rapid mounting and long term storage of slides. Its low viscosity allows for a thinner mounting layer offering better optical quality and bubble-free preparations.

It has a refractive index near that of a fixed protein which helps to keep images free of distortion. Ideal for mounting coverslips to slides with thick or thin specimens Permount preserves most biological stains with little or no fading when the slides are stored in darkness. It contains an anti-oxidant to prevent the formation of annular rings and its high softening point (155°C/311°F) makes it suitable for microprojection.

RT	17986-01	Permount™ Mounting Medium	100ml	24.00
RT	17986-05	Permount™ Mounting Medium	500ml	42.00

*Ownership of Fisher Scientific

Permabond 910

See listing in Specimen Preparation and Embedding Supplies

Tissue-Tack Adhesive

* Good for JB-4 and JB-4 Plus Sections.
* Improves permanent adhesion of sections to the glass slide during prolonged staining procedures and washes.
* Useful for immunofluorescent, immunoperoxidase and routine staining procedures.

RT	71301-01	Tissue-Tack Adhesive	100ml	47.00
RT	71301-05	Tissue-Tack Adhesive	500ml	142.00

UV Mount Medium

* A mounting medium that is designed to match the refractive index of JB-4 embedded sections.
* Cures within 2 minutes when exposed to long wave UV light(365nm) when applied to moist sections.
* Sections are mounted after last staining water rinse.
* Slides must be coverslipped.

0-5ºC

17996

UV Mount Medium

100ml

9900

For More Adhesives

• JB-4 and JB-4 Plus
• Embedding Kits.
• JB-4 Embedding Supplies.

Staining Apparatus