J. NEUROLOGICAL TISSUE STAINS

Method for Supporting Elements

Ptah Method for Central Nervous Tissue

For staining microglia, fibroglia, fibrin and coarse fibrils.

Stain results:

Nuclei, Fibrin, Fibroglia, Microglia:		Blue
Elastic Fibrils, Coarse:		Purplish

References:

• AFIP Manual of Histologic Staining Techniques; (ed) G. Luna, 3rd Ed. McGraw-Hill Publications, c. 1968, p. 199

RT	26658-01	Potassium Permanganate, 1%, Aqueous	500 ml	19.00	Add to Cart
RT	26658-02	Phosphotungstic Acid Hematoxylin (Ptah)	500 ml	46.00	Add to Cart
RT	26658-03	Oxalic Acid, 5%, Aqueous	500 ml	28.00	Add to Cart
RT	26658-04	Lugol’s Iodine Solution	500 ml	26.00	Add to Cart
RT	26658-05	Russel’s Modified Zenker’s Fluid	500 ml	31.00	Add to Cart

Methods for Nerve Cells, NISSL, Granules, Negri Bodies, and Chromatolysis

Vogt’s Method for Nerve Cell Products

Stain results:

Nissl Substance:		Intense Purple
Nuclei:		Purple
Background:		Clear

References:

• AFIP, Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: New York: McGraw-Hill Publications, c. 1968, p. 212

RT	26671-01	Cresyl Violet Acetate, 2%, Aqueous	100 ml	64.00	Add to Cart
RT	26671-02	Acetate Buffer	500 ml	17.00	Add to Cart
RT	26671-1A	Cresyl Violet Acetate Working Solution	250 ml	26.00	Add to Cart

Methods for Myelin Sheaths

Kluver-Barrera Method for Myelin and Nerve Calls

Relation of nerve cells to neutroglia, etc.

Stain results:

Myelin, including phospholipids:		Blue to Green cells
Cells and Cell products:		Pink to Violet

References:

• Kluver, H., and Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Nueropath. Exp. Neurol. 12:400 – 403, 1953
• AFIP, Lab Methods in Histotechnology, ed. Edna B. Prophet, Bob Mills. Jacquelyn B. Arrmyl, Leslie H. Sobin, M.D. Wash, D.C. American Registry of Pathology, c. 1992, p. 94-95.

RT	26681-01	Luxol Fast Blue MBS Solution, 0.1%	23.00	250 ml	Add to Cart
RT	26681-02	Cresyl Violet Acetate Solution, 0.1%, Aqueous	20.00	250 ml	Add to Cart
RT	26681-03	Acetic Acid, 10%, Aqueous	23.00	250 ml	Add to Cart
RT	26681-04	Lithium Carbonate Solution, 0.05%, Aqueous	16.00	250 ml	Add to Cart

Woelcker’s Method for Myelin Sheath

Stain for myelin sheath, glial cells and nucleoli of neurons

Stain results:

Glial Cells, Nucleoli of neurons:		Black
Myelin sheath:		Blue
Background:		Clear

References:

• AFIP: Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 206.

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RT	26684-02	Alcoholic Hematoxylin Stock Solution, 10%	500 ml	86.00	Add to Cart
RT	26684-03	Lithium Carbonate Solution, Saturated Aqueous	500 ml	26.00	Add to Cart

Methods for Nerve Fibers and Nerve Endings

Bodian’s Protargol Method

For neurological tissue stains: Nerve fiber and nerve endings.

Stain results:

Nerve Fibers, Nuclei:		Black
Myelinin, Muscle, Erythrocytes, Lissamine Fast Red Counterstain:		Red
Background-Lissamine Fast Red Counterstain:		Yellow
Background-Aniline Blue Counterstain:		Blue

References:

• Luna, L.G.,(ed), Manual of Histologic Staining Methods of the AFIP 3rd Edition, McGraw-Hill, NY, c 1968, p.195.
• Clark, G., (ed.). Staining Procedures, 3rd Edition, Williams & Wilkens Co., Baltimore, c. 1973, 98.

RT	26693-01	Protargol Solution, 1% Aqueous	500ml	73.00	Add to Cart
RT	26693-02	Cooper Shot	50 g	32.00	Add to Cart
RT	26693-03	Gold Chloride, 1% Aqueous	250ml	152.00	Add to Cart
RT	26693-04	Aniline Blue Solution, or	500ml	52.00	Add to Cart
RT	26693-05	Lissamine Fast Red Solution	500ml	44.00	Add to Cart
RT	26693-06	Oxalic Acid, 2% Aqueous	250ml	16.00	Add to Cart
RT	26693-07	Sodium Thiosulfate, 5% Aqueous	500ml	20.00	Add to Cart
RT	26693-08	Phosphomolybdic Acid, 1% Aqu.	500ml	27.00	Add to Cart
RT	26693-09	Artrazine Solution, 1.5%	500ml	33.00	Add to Cart
RT	26693-10	Hydraquinone Reducing Solution	250ml	20.00	Add to Cart

Hirano-Zimmerman Method for Nerve Cells and Fibers (1962)

For the staining of nerofibrils, dendrites, axis cylinders, senile plaques, etc.

Stain results:

Nucleolus and nuclear membrane:		Black
Neurofibrils, dendrites and axis cylinders:		Black
Cytoplasm of astrocytes and the cytoplasmic membranes of macrophages:		Gray
Senile plaques:		Black

References:

• AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L.Luna: New York: McGraw-Hill Pub., c 1968, p. 198
• Hirano, A. and Zimmerman, H.M., Arch. Neurol., 6:114-122, 1962

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