Questions? 800-523-5874 | [email protected]
- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Data Sheets
Bismuth Stain Kit for Specific En Bloc Staining
EMS #11430
Bismuth Stain Kits for Specific En Bloc Staining Kit consists of:
- 10 x 5 ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)
- 10 x 10 ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0)
Procedures
Staining solution:
Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.
Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Postfixation is carried out with 1% Osmium Tetroxide in 0.05 M Sodium Cacodylate buffer, pH 7.2.
Post-staining is not needed.
Reference
Locke & Huie, (1977). Bismuth staining for Light and Electron Microscopy. Tissue Cell 9, 347.
Additional Technical Data Sheets
Bismuth Stain Kit for Enhancing Contrast in Uranyl Acetate Treated Tissue
Bismuth Stain Kit for Mucosubstances and Polysaccharides