Both sensitivity and the capability to penetrate more readily into the specimen are in general comparable to Ultra Small Immunogold Reagents. Direct visualization of 2nm Immunogold Reagents is possible in TEM on specimens with low electron density or by using e.g. an annular dark field detector in STEM. For ease of visualization silver enhancement may be used. Enhancement with R-Gent SE-EM results in a homogeneous gold/silver particle size population. R-Gent SE-LM is the silver enhancement reagent of choice in light microscopic or immuno blotting experiments

This new series of immunogold reagents have the capacity to fill the gap between Aurion's unique ultra-small gold reagents and the conventional sized 6-25 nm gold conjugates.

Features

• Monodisperse size population
• Coefficient of variance < 15%
• For pre- and post-embedding labeling
• Direct visualization in EM optional
• Homogeneous silver enhancement
• In EM applications; use R-Gent SE-EM
• For LM/bio-assays use R-Gent SE-LM

Aurion 2nm conjugated GAR and GAM IgG are prepared using a monodisperse size population of 2 nm gold nanoparticles. Due to its small gold nanoparticle size, labeling density with these Aurion 2 nm Immuno Gold Reagents is significantly higher compared to particle density obtained with comparable Conventional Immuno Gold Reagents. This makes these 2 nm immunogold conjugates especially suited for detection of low copy numbers of antigen.

The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

The products are available in two package sizes:
0.4 ml for 320 grids or 160 slides
1.0 ml suited for 800 grids or 400 slides

The products should be stored at 4-8°C.

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