"I am so happy. I read on your web site about UranyLESS and I wanted you to know that I think it did a spectacular job on this sample!" - Debra Townley, Baylor College of Medicine

Substitute for Uranyl Acetate

EMS is proud to introduce UranyLess, a new contrast stain solution for TEM, for all of your negative staining applications. It is an amazing substitute for Uranyl Acetate with similar results.

After only a minute of contact, UranyLess' fast-acting, non-radioactive lanthanide mix is finished staining your sections or deposits (see protocols below). If needed, lead citrate is recommended to increase the contrast. TIP: To prevent precipitates, use room temperature distilled water.

UranyLess's pH level is about 6.8-7. The 30 ml airless bottle will stain approximately 1500 grids. The airless bottle increases shelf life, eliminates CO₂ contamination, and produces less waste - the solution pumps out in perfect amounts without leaking or spilling. UranyLess is also available in a larger amount for use in automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.

UranyLess has been tested on many biological tissue (animal and plant): intestine, skeletal and cardiac muscle, liver, kidney, adrenal gland, nerve, cell culture, plant tissue, and also on negative staining of bacteriophage, bacteria, and polymers. UranyLess is ideal because of its ability to stain any kind of material and results are reproducible. To see the effectiveness of Uranyless, please visit the UranyLess Image Gallery.

• Classic Staining Protocol
• Negative Staining Protocol
• Technical FAQs

UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess / Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate 3%, ready to use. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO₂ dissolution. It is convenient to use because it performs in any position, even upside down. Comes in either a 30 ml Airless bottle or a 30 ml Airless Syringe. The Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100

References:

"Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules." Benmeradi N1,2, Payre B2 and Goodman SL3.

1. Delta Microscopies, 22 bis, route de Saint Ybars, 31190 Mauressac, France
2. Université Toulouse, CMEAB Faculté Medecine, 118 route Narbonne, 31062, Toulouse, France
3. Microscopy Innovations LLC, 213 Air Park Rd, Suite 101, Marshfield, WI, 54449, USA

"C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle." Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.

UranyLess Applications