Aurion Anionic Gold Tracers are used to visualize charged moieties in specimens. They bind to polycationic moieties (basic proteins e.g. histones). "Neutral" BSA coated gold tracers are useful for detecting open connections and tissue damage. Under proper conditions and with suitable microscopical techniques, the tracers can also be used to follow cellular events in time.

Introduction

The AURION Anionic Gold Tracers are designed to detect multiple positive charge moieties.

Product description

The AURION Gold Tracers are available in the full range of particle sizes: Ultra Small, 6, 10, 15 and 25 nm. Anionic Gold Tracers are prepared with BSA-c™ as particle conjugated protein. AURION Gold Tracers are available in 5 and 10 ml volume packages and are supplied at an OD520nm of 2.0 for the conventional particle size range and at equivalent OD for the Ultra Small tracers.

The products are supplied in PBS, with 15 mM NaN₃.

Note 1 - AURION Gold Tracers are also available in bulk and at different optical density if required.

Note 2 - AURION Gold Tracers are shipped containing NaN₃ as preservative. If they are intended for use in living organisms, the preservative has to be removed prior to use. This can be achieved either by dialysis or by buffer exchange using, for instance, a Pharmacia PD-10 column.

Storage

Aurion gold tracers have a guaranteed shelf life of 18 months from the date of quality control analysis.

The products should be stored at 4-8°C. Freezing is not recommended.

Technical Tip

Gold Tracers Application Instructions

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