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Silver Enhancement Reagents

The size of Ultra Small gold particles can be increased by silver enhancement allowing their visualization at electron and light microscopy levels. This process involves the gold particle mediated reduction of silver ions and deposition of metallic silver on the particle surface.

Aurion provides two types of silver enhancement reagents, AURION R-Gent SE-EM and AURION R-Gent SE-LM.
AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy applications. It is characterized by its homogeneous enhancement, light insensitivity, low viscosity, near neutral pH and virtually no autonucleation.
AURION R-Gent SE-LM, can be used for light microscopy and blotting applications. It produces a brown to black enhancement when viewed by bright field light microscopy. Furthermore, it is light insensitive, has near neutral pH and extremely low autonucleation.

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Aurion Immunogold Ultra Small Reagents
 
Aurion_Silver_Micrograph_2.jpg
Post embedding immunolabeling: Detection of Lamp2 on Tokuyasu sections of 4% PFA fixed HeLa cells using AURION GAM IgG 2nm and AURION R-Gent SE-EM. Courtesy of M. Mari, UMCG & RUG, Molecular Cell Biology, Groningen, The Netherlands
 
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R-Gent Silver Enhancement Reagents, SE-LM

Intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected in electron microscope.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 25520 Description: R-Gent Silver Enhance (2X30mL) SE-LM Kit Pack: 2 x 30 mL Price: $159.00 Add to Quote:
Storage:4-8°C Cat #: 25522 Description: R-Gent Silver Enhance (2X250mL) SE-LM Kit Pack: 2 x 250 mL Price: $235.50 Add to Quote:

R-Gent Silver Enhancement Reagents, SE-EM

Intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected in electron microscope.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 25521 Description: R-Gent SE-EM Pack: 30 mL Price: $252.50 Add to Quote:
Storage:4-8°C Cat #: 25521-90 Description: SE-EM Kit Pack: 90 mL Price: $503.50 Add to Quote:

ECS Enhancement Conditioning Solution

Helps optimize enhancement by conditioning specimens labeled with (ultra small) gold conjugates before the silver enhancement procedure using Aurion R-Gent SE-EM.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 25830 Description: ECS Enhancement Conditioning Solution Pack: 100 mL Price: $63.50 Add to Quote: