EMS Shield Mount with antifading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous non-permanent, low viscosity mounting medium for immunofluorescence. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine and Redox. The fluorescence is retained during storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes.
Immunofluorescence, confocal microscopy.
Ready to use mounting medium for Immunofluorescence.
2-8°C is recommended. Protect from light, DO NOT FREEZE.
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deionized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.