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- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Data Sheets
Harris Hematoxylin
EMS Catalog Number: 26041-05 and 26041-06
Fixation
Follow most fixatives.
Sections
Paraffin
Staining
- Deparaffinize and hydrate to distilled water..
- Stain in Harris Hematoxylin containing 2 – 4 ml of Glacial Acetic Acid per 100 ml for 5 15 minutes. Wash in water.
- Differentiate in Acid Alcohol until the sections turn red.
- Blue section in Sodium Acetate, 1% or Ammonia Water, 0.3%, or Lithium Carbonate Solution
- Wash thoroughly and counterstain.
References
Harris, H.F., J. Appl. Microscopy, III: 777-781, 1900
Mallory, F. B., Pathological Technique, W. B. Saunders Co., Philadelphia, 1938.
Clark, G., (ed.), Staining Procedures, 3rd Edition, William & Wilkins, Baltimore, pp33 & 35, c 1973.
Luma, L.G., (ed.) Manual of Histologic Staining Method of the AFIP, 3rd edition, McGraw-Hill, NY, pp 34 & 38, c1968.