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EMS Technical Data Sheets
Carstairs' Method for Fibrin & Platelets
EMS Catalog #: 26381-Series
Fixation:
Formalin-Saline for 48 hours or more.
Sections:
Cut paraffin at 5 µm sections.
Staining Procedures:
- Hydrate slides to distilled water.
- Mordant in Ferric Ammonium Sulfate, 5% (#26381-01) for 5 minutes. Rinse in running tap water.
- Stain with Mayer Hematoxylin (#26381-02) for 5 minutes. Rinse in running tap water.
- Stain in the Picric Acid-Orange G Solution (#26381-03) for 30 minutes to 1 hour. Rinse once in distilled water.
- Stain in Ponceau Fuchsin Solution (#26381-04) for 1 to 5 minutes. Rinse in distilled water.
- Differentiate with Phosphotungstic Acid, 1% (#26381-05) until the muscle is red and background pale pink. Rinse in distilled water.
- Stain with Aniline Blue Solution (#26381-06) for 30 minutes to 2 hours. Rinse in several changes of distilled water.
- Dehydrate, clear in Xylene and coverslip, using a synthetic mounting medium.
Stain Results
| Fixation Time: | 48 Hours or more | Less than 48 hours |
| Fibrin | Bright Red | Orange to Orange Red |
| Platelets | Gray Blue or Navy | Light Gray |
| Collagen | Bright Blue | Bright Blue |
| Muscle | Red | Red |
| Red Blood Cells | Clear Yellow | Red, Green or Yellow |
Product Information
Carstairs' Method for Fibrin & Platelets