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EMS Technical Data Sheets
Dane's Method for Prekeratin, Keratin, & Mucin
EMS Catalog #: 26401-Series
Fixation:
10% Buffered Neutral Formalin (#15740)
Sections:
Cut paraffin at 6 µm sections.
Staining Procedures:
- Deparaffinize and hydrate slides to distilled water.
- Mayer Hematoxylin (#26401-04) for 10 minutes. Blue in running tap water for 10 minutes. Rinse in distilled water.
- Phloxine B 1% Solution (#26401-01) for 3 minutes. Wash in running tap water to remove excess stain. Rinse in distilled water.
- Alcian Blue, 0.5% (#26401-02) for 5 minutes. Wash in tap water for 2 minutes. Rinse in distilled water.
- Orange G Solution (#26401-03) for 13 minutes.
- Transfer to 95% alcohol, two changes, five dips each; absolute alcohol, two changes, fifteen dips each.
- Clear in Xylene and mount.
Stain Results:
| Acid Mucopolysaccharides | Blue |
| Prekeratin and Keratin | Orange to Red |
| Nuclei | Brown |
References:
Dane, E.T., and Herman, D. L.: Stain Techn., 38:97, 1963
AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: NY, McGraw-Hill Publ., c. 1968, p.83.
Product Information
Dane's Method for Prekeratin, Keratin, & Mucin