Questions? 800-523-5874 | [email protected]
- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Data Sheets
Maldonado's Method For Pancreatic Islet Cells
EMS Catalog #26458
Fixation
Bouin's without Acetic Acid
Section
Paraffin, @ 6 Microns
Staining Procedure
- Deparaffinize and hydrate to distilled water.
- Stain for 10 minutes in Phloxine Solution, 1% (26202-01) Rinse in distilled water.
- Place for 1 minute in Phosphotungstic Acid, 3% (26458-02). Rinse in distilled water.
- Stain for 30 seconds in Azure II Solution, 0.05% (26458-03) Rinse in distilled water.
- Stain in Weigert's working solution for 1 minute. Rinse in distilled water.
To prepare Weigert's Working solution:
Mix just before use:
1 part Weigert's A (26458-04)
1 part Weigert's B (26458-05) DO NOT REUSE!!!!! - Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.
- Mount with Permount (17985)
Results
|
Human Tissue
|
|
| Alpha granules: |
Purple
|
| Beta granules: |
Violet Blue
|
| D-cell granules: |
Light blue with evident granules
|
| Exocrine cells: |
Grayish blue with red granules
|
References
Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967
Luna , L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y.
p. 105, c. 1968
Product Information
Phloxine Solution, 1%
Phosphotungstic Acid, 3%
Azure II Solution, 0.05%
Weigert's A
Weigert's B
Maldonado's Method For Pancreatic Islet Cells