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EMS Technical Data Sheets
Fat Stains In Supersaturated Alcohol
EMS Catalog #: 26503-Series
Fixation:
Formalin.
Sections:
Frozen
Preparing Working Stain
Dilute 6 ml of stock solution Sudan IV in Isopropanol or Oil Red O in Isopropanol or Sudan III Isopropanol solution with 4 ml of distilled water. Allow to stand for 5-10 minutes and then filter. The filtrate may be used for several hours
Procedure
- Stain thin frozen sections with the working fat stain for 10 minutes. Wash in tap water.
- Stain for 5 minutes in either Mayer’s Hematoxylin or Ehrlich’s Hematoxylin diluted.
- Place in Sodium Phosphate 1 %, or in tap water until blue.
- Float out in water, take up on a slide and mount on Apathy’s Gum Syrup or Zwemer’s Glychrolgel or any other soluble aqueous medium.
Stain Results:
| Nuclei | Blue |
| Fat | Orange-Red |
| Erthrocytes | Sometimes Green |
| Cytoplasm | Lighter Green |
References:
Clark, G, ed.: Staining Procedures, 3rd ed.: Baltimore: Williams & Wilkins Co., 1973, p. 149