Questions? 800-523-5874 | [email protected]
- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Data Sheets
Nile Blue A
EMS Catalog #26511
Fixation
Formalin fixatives
Application
Show differentiation of melanins and lipofuscins
Staining
- Deparaffinize and hydrate to distilled water.
- Stain 20 minutes in Nile Blue A Staining solution.
- Rinse quickly with sulfuric acid, 1%.
- Dehydrate immediately in four changes of acetone, 15 seconds each. Rinse in Xylene-acetone. Clear in two changes of Xylene.
- Mount.
Stain Results
| Lipofuscins | Unstained - natural yellow |
| Melanins | Green |
References
Staining Procedures, G. Clark: Williams and Wilkins Co., Baltimore, 3 rd ed., c. 1973, p. 122.
Lillie, R.D.: Stain Techn ., 31:151.