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General approach is 1 min of each station from 1 to 7 per 1x1mm of tissue thickness(size), so if tissue is 1x1mm it should be kept in every station for 1 minutes(see below)
In case you want to perform even faster microscopic analysis of the specimen you can increase the temperature of all Stations (steps) from 1 to 7.
Temperature of all Stations (1-7) with processing solutions should be kept at 46-50° Celsius.
Temperature of paraffin stations should be kept at 60° Celsius.
In case of troubleshooting, please refer to routine formalin processing adjusting to processor time
P# | Station | Cutting water bath temp | |||||||||||
1 PPS1 |
2 PPS2 |
3 PPS3 |
4 PPS4 |
5 PPS4 |
6 PPS5 |
7 PPS5 |
8-10 Skip |
11 Paraffin |
12 Paraffin |
13 Paraffin |
14 Skip (Paraffin) |
||
1 | 2 min. |
2 min.2 min.2 min.2 min.2 min.2 min.Skip2 min.2 min.2 min.Skip36-40°C
23 min.3 min.3 min.3 min.3 min.3 min.3 min.Skip3 min.3 min.3 min.Skip36-40°C36 min.6 min.6 min.6 min.6 min.6 min.6 min.Skip6 min.6 min.6 min.Skip36-40°C42 min.2 min.2 min.2 min.2 min.2 min.2 min.Skip5 min.10 min.10 min.Skip30-36°C53 min.3 min.3 min.3 min.3 min.3 min.3 min.Skip5 min.10 min.10 min.Skip30-36°C64 min.4 min.4 min.4 min.4 min.4 min.4 min.Skip5 min.10 min.10 min.Skip36-40°C75-10 min.5-10 min.5-10 min.5-10 min.5-10 min.5-10 min.5-10 min.Skip5 min.10 min.10 min.Skip36-40°C83 min.3 min.3 min.3 min.3 min.3 min.3 min.Skip5 min.5 min.5 min.Skip46-47°C910-15 min.10-15 min.10-15 min.10-15 min.10-15 min.10-15 min.10-15 min.Skip10-15 min.10-15 min.10-15 min.Skip25-30°C