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Capillary Blood Or Heparinized Venous Blood Films
Intensity of precipitated dye in the cytoplasm of neutrophilic leukocytes varies. One hundred are scored from 0 to 4+ based on the intensity of the precipitated dye.
CELL RATING | AMOUNT **% | Ppt'd azo dye In cytoplasm Size | INTENSITY | BACKGROUND OF CYTOPLASM |
1+ | <50 | Small | Faint to moderate | Colorless to very pale pink |
2+ | 50-80 | Small to Med. | Moderate to strong | Colorless to pale pink |
3+ | 80-100 | Med. To Large | Strong | Colorless to pink |
4+ | 100 | Med. To Large | Brilliant | Not visible |
* When there is no precipitate azo dye in the cytoplasm and the background is not stained, the cells are read as zero.
** Percentage of volume of cytoplasm occupied by azo dye precipitate.
Adults - Leukocyte Alkaline Phosphatase Score Of 13 To 130.
Williams, W.J., Beutler, El, Erslev, A.J. and Rundles R.W., Hematolog y, 2 nd ed., McGraw-hill , NY , C. 1977, p1628-1629.
Kaplow, L.S., AM.J., Clin. Patbol ., 39:439, 1963.
Kaplow, L.S. and Burstone, M.S., Nature , 200:690, 1963.
Buffered Acetone Fixative
Naphthol AS-BI Phosphate
Dimethyl Formamide
Fast Red Violet Salt LB
Fast Violet B Salts
Propanediol Buffer
Hematoxylin Counter Stain
Leukocyte Alkaline Phosphatase