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EMS Technical Data Sheets
Cellometer™
EMS Catalog #63560-25</h2
The Disposable Cell Counting Chamber
These Disposable Cell Counters are an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.
Figure 1: Schematic Drawing of a Cellometer TM
The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
Figure2: Counting Grid: The Liquid Volume
Under square A, B, C are 1x10-4 ml, and 2.5x10-7 ml respectively.
Procedure For Use
- Pipette 15-20 microliters cell suspension through sample port.
- Place Cellometer under optical microscope.
- Adjust focus on both the grid lines and the cells.
- Count cells following standard cell counting procedures.
- Count the total number of cells found in four of the large corner squares as shown in Figure 2. (Total Count)
- Calculate cell concentration (Cell concentration= Dilution factor x (total count ÷ 4) x 10 4
- Dispose cell counting chamber in bio waste.
Cellometer™ with cells