Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
2-8°C, DO NOT FREEZE
Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
This enzyme is supplied as concentrated form along with buffer.
To one ml of buffer (reagent B), add 2-4 drops of concentrated enzyme (reagent E) in a test tube, mix well. This ready-to-use reagent is good for 2-3 hours.
The unused solution can be discarded according to city, county, state, province or country’s regulations.
Limitation: Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.
This product contains 0.05% Proclin 300 as a preservative, appropriate care should be taken in handling. Avoid skin and eye contact with all laboratory products. Use appropriate laboratory gear, laboratory coat, gloves and safety glasses. Do not ingest any laboratory products. This product is not approved for administration in human or animals.
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Country of origin: USA